Effect ofP CO2, bicarbonate and lactate on the isometric contractions of isolated soleus muscle of the rat

Pannier, Jean; Weyne, J; Leusen, I

doi:10.1007/bf00588547

Cited by 33 publications

(18 citation statements)

References 46 publications

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“…Isolated muscles composed of widely differing fibre types showed, with elevated CO2 concentrations in bathing or perfusing solutions, a decrease in isometric tension (Fretthold & Garg, 1978;Sahlin et al 1983;Spriet, Matsos, Peters, Heigenhauser & Jones, 1985;Ranatunga, 1987;Renaud, Stein & Gordon, 1987). In contrast to these results, an increase in tension of soleus and EDL with increased CO2 concentration was reported at a higher temperature of the bathing solutions (Pannier et al 1970;Ranatunga, 1987). With metabolic acidosis obtained by low bicarbonate a decrease in isometric tension (Pannier et al 1970;Fretthold & Garg, 1978;Spriet et al 1985) has been documented.…”

Section: Resultsmentioning

confidence: 86%

“…With metabolic acidosis obtained by low bicarbonate a decrease in isometric tension (Pannier et al 1970;Fretthold & Garg, 1978;Spriet et al 1985) has been documented. In comparative studies, Spriet et al (1985) concluded that there was no difference between moderate respiratory and metabolic acidosis in force developed by rat hindlimb muscles, whereas Pannier et al (1970) reported a decrease in tension with low bicarbonate and an increase in tension with elevated C02 concentrations in soleus.…”

Section: Resultsmentioning

confidence: 99%

“…Fabiato & Fabiato (1978) showed that the pH optimum for Ca2+ loading of the sarcoplasmic reticulum is more acidic in skeletal than in cardiac muscle of the frog and concluded that in a moderate acidosis the increased Ca2+ loading and thus larger amount of Ca2+ released in skeletal muscle will compensate for the effect of low pH on the myofilaments. A predominant effect of a moderate acidosis on the sarcoplasmic reticulum may explain the observation of Pannier, Weyne & Leusen (1970) that a moderate increase in PCO2 causes an increase rather than a decrease in isometric tension of the soleus muscle.…”

Section: Isometric Force Developmentmentioning

confidence: 98%

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Effects of carbonic anhydrase inhibitors on contraction, intracellular pH and energy‐rich phosphates of rat skeletal muscle.

Geers

Gros

1990

The Journal of Physiology

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SUMMARY1. The effects of carbonic anhydrase inhibitors on contractile parameters, intracellular pH (pHi) and energy-rich phosphates were studied in isolated rat soleus and extensor digitorum longs (EDL) muscles.2. The muscles were incubated either in Ringer solutions (95% 02/5% CO2 = control) or in solutions to which one of the inhibitors, 5 x 10' M-chlorzolamide or 10-2 m-NaCNO, had been added. Muscles were stimulated directly and contracted under isometric conditions.3. Compared with control muscles, both inhibitor-treated muscles showed a significantly decreased tetanic force and an increased half-relaxation time of twitches and tetani. Chlorzolamide increased time-to-peak in both muscles. Cyanate decreased isometric twitch force in both muscles.4. Both inhibitors decreased pHi in both muscles; chlorzolamide by 0.1 unit, cyanate by 0-4 unit in soleus and by 0-8 unit in EDL.5. Chlorzolamide increased the concentrations of creatine and inorganic phosphate (Pi) in soleus (the effect of chlorzolamide was not studied in EDL). Cyanate caused these same changes in soleus as well as EDL and in addition decreased the concentrations of ATP and phosphocreatine in soleus and EDL.6. In muscles acidified by either low external HCO3-(2 mM) or by elevated Pco2(30 % CO2 in the gas phase) in the bath, decreases in isometric force and increases in half-relaxation time of tetani were observed. In addition there were increases in muscle Pi. These effects were more pronounced with 30% CO2 than with 2 mM-HCO3-. 7. Neither acidifying solutions prolonged either half-relaxation time or time-topeak of twitches.8. We conclude that carbonic anhydrase inhibition exerts its effect (a) on isometric tension at least partly via an elevated Pi (perhaps in combination with lowered pHi);(b) on the half-relaxation time of tetani by means of lowered pHi and elevated concentration of Pi; (c) on relaxation and time-to-peak of twitches by some unknown mechanism, neither directly by a change in pHi nor in Pi.MS 7639

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Section: Resultsmentioning

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Section: Isometric Force Developmentmentioning

confidence: 98%

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Effects of carbonic anhydrase inhibitors on contraction, intracellular pH and energy‐rich phosphates of rat skeletal muscle.

Geers

Gros

1990

The Journal of Physiology

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“…(ii) Skeletal muscle shows little sensitivity to changes in pHo (Pannier, Weyne & Leusen, 1970) though the calcium sensitivity of the contractile proteins is affected by pH .…”

Section: Introductionmentioning

confidence: 99%

The effects of changes of pH on intracellular calcium transients in mammalian cardiac muscle.

Allen¹,

Orchard²

1983

The Journal of Physiology

199

120

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monophasic decline in developed tension, and a small increase in peak light. Alkaloses produced by changing the [HC03-]/[C02] ratio produced similarresults but the changes observed were in the opposite direction to those described above.6. The effects of changes of pHo can be explained if pHi affects tension by two mechanisms. The first mechanism, which is responsible for the rapid change in tension, is not associated with a change in [Ca2+]j. The second mechanism leads to a slower and smaller change in tension, in the opposite direction to the first, and is due to a change in the intracellular calcium transient.

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“…Further, the effects of elevated CO 2 on skeletal muscle force generation are equivocal. Hypercapnia has been shown to potentiate (Pannier et al 1970), impair (Barclay 1987;Mador et al 1997;Vianna et al 1990), or have no effect (Foulks and Perry 1977) on skeletal muscle force generation. Additionally, ACZ treatment and associated hypercapnia did not alter maximal voluntary torque production (Brechue et al 1997).…”

Section: Introductionmentioning

confidence: 98%

Hypercapnic impairment of neuromuscular function is related to afferent depression

Beekley

Cullom

Brechue

2004

European Journal of Applied Physiology

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Acetazolamide (ACZ), a carbonic anhydrase inhibitor, results in altered neuromuscular function secondary to depressed afferent transmission in intact humans. One effect of ACZ is hypercapnia. Thus, to test if the neuromuscular depression observed following ACZ treatment is related to elevated CO(2), human subjects ( n=10) were exposed to 15 min of room air (0% CO(2)) or hypercapnia (7% inspired CO(2)), and neuromuscular function was evaluated. Isometric force (36.8 to 31.1 N) and peak-to-peak electromyographic amplitude (EMG, 1.5 to 1.0 mV) associated with an Achilles tendon tap, and soleus H(max):M(max) ratio (69.0 to 62.2%) were depressed, while EMG latency (34.8 to 39.8 ms) was increased by hypercapnia. Reflex recovery profiles (following a conditioning tap to the contralateral Achilles tendon), motor nerve conduction velocity, amplitude of the maximum M-wave, and peak twitch tension at M(max) were unaltered by hypercapnia. We conclude that elevated CO(2) impairs neuromuscular function through effects on afferent transmission or synaptic integrity between type Ia fibers of the muscle spindle and the alpha motor neuron, without affecting the muscle spindle, efferent conduction or skeletal muscle force-generating capacity.

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Effect ofP CO2, bicarbonate and lactate on the isometric contractions of isolated soleus muscle of the rat

Cited by 33 publications

References 46 publications

Effects of carbonic anhydrase inhibitors on contraction, intracellular pH and energy‐rich phosphates of rat skeletal muscle.

Effects of carbonic anhydrase inhibitors on contraction, intracellular pH and energy‐rich phosphates of rat skeletal muscle.

The effects of changes of pH on intracellular calcium transients in mammalian cardiac muscle.

Hypercapnic impairment of neuromuscular function is related to afferent depression

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