Effect on Platelet FXIII and Partial Characterization of Lonomin V, a Proteolytic Enzyme from Lonomia achelous Caterpillars

Guerrero, Belsy; Perales, Jonás; Gil, Amparo; Arocha-Piñango, Carmen Luisa

doi:10.1016/s0049-3848(98)00169-8

Cited by 24 publications

(17 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, this decrease in plasma factor XIII in plasma of envenomed patients, after contact with L. achelous and L. obliqua caterpillars seems to be a consequence of different mechanisms. The venom of L. achelous contains one well-characterized substance that degrades human factor XIII in vitro (10)(11)(12)37). This substance, called Lonomin V, produces a dose-dependent thrombolysis in combination with a decrease in factor XIII level (37).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Antithrombotic effect of Lonomia obliqua caterpillar bristle extract on experimental venous thrombosis

Prezoto

Maffei

Mattar

et al. 2002

Braz J Med Biol Res

View full text Add to dashboard Cite

The venom of Lonomia obliqua caterpillar may induce a hemorrhagic syndrome in humans, and blood incoagulability by afibrinogenemia when intravenously injected in laboratory animals. The possible antithrombotic and thrombolytic activities of L. obliqua caterpillar bristle extract (LOCBE) were evaluated in this study. The minimal intravenous dose of the extract necessary to induce afibrinogenemia and anticoagulation was 3.0 and 10.0 µg protein/kg body weight for rabbits and rats, respectively. In rabbits, this dose induced total blood incoagulability for at least 10 h and did not reduce the weight of preformed venous thrombi, in contrast to streptokinase (30,000 IU/ kg). In rats, pretreatment with 5.0 and 10.0 µg/kg LOCBE prevented the formation of thrombi induced by venous stasis or by injury to the venous endothelium. The dose of 5.0 µg/kg LOCBE did not modify blood coagulation assay parameters but increased bleeding time and decreased plasma factor XIII concentration. When the extract was administered to rats at the dose of 10.0 µg/kg, the blood was totally incoagulable for 6 h. These data show that LOCBE was effective in preventing experimental venous thrombosis in rats, justifying further studies using purified fractions of the extract to clarify the mechanisms of this effect.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Arocha-Piñango et al (4,5) have published several studies on aspects of L. achelous envenomation, common laboratory findings in envenomed patients (6,7), and on the effects of the venom and fractions derived from the venom on coagulation and fibrinolysis (8)(9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Antithrombotic effect of Lonomia obliqua caterpillar bristle extract on experimental venous thrombosis

Prezoto

Maffei

Mattar

et al. 2002

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

“…Process costs can be reduced by either replacing certain components of the culture medium, which will result in high cell densities at lower costs and less operation complexity, or by using substances which promote cell growth, cell protection or even cell productivity. Some studies have demonstrated the presence of pharmacologically active substances in insect hemolymph (Shiotsuki et al 2000;Yamamoto et al 1999;Guerrero et al 1999;Jiang et al 1999;Rosenfeld and Vanderberg 1998;Hamdaoui et al 1998;Maranga et al 2003;Huberman et al 1979;Lin et al 1998;Kurata et al 1994;Jones et al 1993;Zhu et al 2000;Lowenberger et al 1999;Lamberty et al 1999;Gross et al 1998;Johns et al 1998;Lanz-Mendoza et al 1996;Peters et al 1993;Souza et al 2005). The supplementation of cultures with hemolymph proteins has had a positive effect on viral replication (Souza et al 2005;Rhee and Park 2000;Kanaya and Kobayashi 2000) and recombinant protein production (Woo et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Enhancing effect of a protein from Lonomia obliqua hemolymph on recombinant protein production

et al. 2008

View full text Add to dashboard Cite

Gene expression in animal cells allows large scale production of proteins used for either structure and function studies or therapeutic purposes. Maximizing recombinant protein production is necessary to optimize cell growth and protein expression. Some studies have demonstrated the presence of pharmacologically active substances in insect hemolymph. In this work, we have identified and purified a protein from Lonomia obliqua hemolymph able to increase the production of the rabies virus glycoprotein, expressed in Drosophila melanogaster S2 cells, by about 59%.

show abstract

“…Other compounds were isolated from fluids of L. achelous , including direct fibrinolytic, plasminogen activator toxins, as well as toxins that activate factors II, V and XIII [65][66][67] . In that case, antifibrinolytic drugs could neutralize either fibrinolytic toxins or the primary activation of fibrinolysis [66] .…”

Section: Secondary Activation Of Fibrinolysismentioning

confidence: 99%

New Insight into the Mechanism of <i>Lonomia obliqua</i> Envenoming: Toxin Involvement and Molecular Approach

Alvarez-Flores

Zannin²,

Chudzinski-Tavassi³

2010

Pathophysiol Haemos Thromb

View full text Add to dashboard Cite

Despite the nearly worldwide distribution of Lepidoptera, there are few species with clear documentation of adverse reactions in humans. Most syndromes caused by Lepidoptera are consequences of direct contact with the hairs or setae of caterpillars. In most instances, the adverse effects caused by moth and caterpillars are self-limited and the treatment is based on the removal of hairs, application of topical antipruritics and, in some cases, the use of oral antihistamines. However, in the case of envenoming by South American Lonomiaobliqua caterpillars, the antilonomic serum produced at Instituto Butantan in Brazil is the only effective treatment to re-establish the physiological coagulation parameters in poisoned patients and to abolish the complications seen in severe cases (e.g. consumptive coagulopathy, intracerebral hemorrhage, and acute renal failure). Many studies have been carried out to understand the pathophysiological mechanism of envenoming by L. obliqua. Several toxic principles were found in bristle extract and the hemolymph, probably related to the envenoming. An interesting fact is that some toxins from the venom usually have more than one function. With the advent of molecular biology techniques it has become possible to analyze these processes at a molecular level, thus giving rise to hypotheses on the molecular basis of envenomation. This review contributes to enhance our understanding of the dramatic alterations that hemorrhagic syndrome causes in patients, current treatment, and the diversity of the molecules involved in this pathology.

show abstract

Effect on Platelet FXIII and Partial Characterization of Lonomin V, a Proteolytic Enzyme from Lonomia achelous Caterpillars

Cited by 24 publications

References 24 publications

Antithrombotic effect of Lonomia obliqua caterpillar bristle extract on experimental venous thrombosis

Antithrombotic effect of Lonomia obliqua caterpillar bristle extract on experimental venous thrombosis

Enhancing effect of a protein from Lonomia obliqua hemolymph on recombinant protein production

New Insight into the Mechanism of <i>Lonomia obliqua</i> Envenoming: Toxin Involvement and Molecular Approach

Contact Info

Product

Resources

About