This work presents the results of an investigation into the influence of beam offset on the structure and mechanical properties of electron-beam-welded joints between Ti6Al4V and Al6082-T6 alloys. The experimental procedure involved the use of specific technological conditions: an accelerating voltage of 60 kV, an electron beam current of 35 mA, a specimen motion speed of 10 mm/s, and a beam offset of 0.5 mm towards both alloys, as well as welding without an offset. The phase composition of the joints was analyzed using X-ray diffraction (XRD). The microstructure and chemical composition of the seams were studied by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). The results obtained for the structure of the joints show that the beam offset has a significant influence on the structure. The microhardness was studied by means of the Vickers method. The results for the microstructure showed that the welding procedure without offset and with an offset towards the Ti alloy leads to inhomogeneous welded joints with a significant amount of intermetallics. The offset towards the Al alloy leads to the formation of a narrow area of TiAl3 phase. The measured microhardness corresponds to the increased amount of intermetallics in the case of offset towards the Ti alloy, with which the highest values were presented (about 58% higher than with Ti6Al4V plate). The results obtained for tensile properties show that the offset to the Al6082-T6 alloy leads to the highest values of tensile strength (TS) and yield strength (YS), which are twice higher than in welding without offsetting of the electron beam.