Effect on tumorigenicity and metastasis of transfection of a diploid benign rat mammary epithelial cell line with DNA corresponding to the mRNA for basic fibroblast growth factor

Davies, Barry R.; Fernig, David G.; Barraclough, Roger; Rudland, Philip S.

doi:10.1002/(sici)1097-0215(19960103)65:1<104::aid-ijc18>3.0.co;2-g

Cited by 9 publications

(4 citation statements)

References 15 publications

(21 reference statements)

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“…Thus, when FGF-2 mRNA levels were analysed in a cohort of breast cancer patients, elevated expression of FGF-2 mRNA correlated with a good prognostic outcome, the opposite of the result expected from the naïve perspective that "FGF2 = uncontrolled growth + angiogenesis = cancer" (Anandappa et al 1994). Similarly, in a syngeneic rat model of breast cancer, overexpression of FGF2 failed to produce any metastases (Davies et al 1996). Given the difficulty in publishing negative results, there is likely a very large body of work that demonstrates the absence of a direct association between the expression of FGF ligands and cancer.…”

Section: The Aspects Of Fgf Activities Linked To Cancersmentioning

confidence: 99%

Peer Review #2 of "Fibroblast growth factors as tissue repair and regeneration therapeutics (v0.1)"

Grose¹

2016

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Cell communication is central to the integration of cell function required for the development and homeostasis of multicellular animals. Proteins are an important currency of cell communication, acting locally (auto-, juxta-, or paracrine) or systemically (endocrine). The fibroblast growth factor (FGF) family contributes to the regulation of virtually all aspects of development and organogenesis, and after birth to tissue maintenance, as well as particular aspects of organism physiology. In the West, oncology has been the focus of translation of FGF research, whereas in China and to an extent Japan a major focus has been to use FGFs in repair and regeneration settings. These differences have their roots in research history and aims. The Chinese drive into biotechnology and the delivery of engineered clinical grade FGFs by a major Chinese research group were important enablers in this respect. The Chinese language clinical literature is not widely accessible. To put this into context, we provide the essential molecular and functional background to the FGF communication system covering FGF ligands, the heparan sulfate and Klotho co-receptors and FGF receptor (FGFR) tyrosine kinases. We then summarise a selection of clinical reports that demonstrate the efficacy of engineered recombinant FGF ligands in treating a wide range of conditions that require tissue repair / regeneration. Alongside, the functional reasons why application of exogenous FGF ligands does not lead to cancers are described. Together, this highlights that the FGF ligands represent a major opportunity for clinical translation that has been largely overlooked in the West.

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Section: The Aspects Of Fgf Activities Linked To Cancersmentioning

confidence: 99%

Peer Review #2 of "Fibroblast growth factors as tissue repair and regeneration therapeutics (v0.1)"

Grose¹

2016

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“…Thus, when FGF-2 mRNA levels were analysed in a cohort of breast cancer patients, elevated expression of FGF-2 mRNA correlated with a good prognostic outcome, the opposite of the result expected from the naïve perspective that "FGF2 = uncontrolled growth + angiogenesis = cancer" (Anandappa et al 1993). Similarly, in a syngeneic rat model of breast cancer, overexpression of FGF2 failed to produce any metastases (Davies et al 1996). Given the difficulty in publishing negative results, there is likely a very large body of work that demonstrates the absence of a direct association between the expression of FGF ligands and cancer.…”

Section: The Aspects Of Fgf Activities Linked To Cancersmentioning

confidence: 99%

Fibroblast growth factors as tissue repair and regeneration therapeutics

Nunes

Sun

et al. 2015

Preprint

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Cell communication is central to the integration of cell function required for the development and homeostasis of multicellular animals. Proteins are an important currency of cell communication, acting locally (auto-, juxta-, or paracrine) or systemically (endocrine). The fibroblast growth factor (FGF) family contributes to the regulation of virtually all aspects of development and organogenesis, and after birth to tissue maintenance, as well as particular aspects of organism physiology. In the West oncology has been the focus of translation of FGF research, whereas in China and to an extent Japan a major focus has been to use FGFs in repair and regeneration settings. These differences have their roots in research history and aims. The Chinese drive into biotechnology and the delivery of engineered clinical grade FGFs by a major Chinese research group were important enablers in this respect. The Chinese language clinical literature is not widely accessible. To put this into context, we provide the essential molecular and functional background to the FGF communication system covering FGF ligands, the heparan sulfate and Klotho co-receptors and FGF receptor (FGFR) tyrosine kinases. We then summarise a selection of case reports that demonstrate the efficacy of engineered recombinant FGF ligands in treating a wide range of conditions that require tissue repair / regeneration. Alongside the functional reasons why application of exogenous FGF ligands does not lead to cancers are described. Together, this highlights that the FGF ligands represent a major opportunity for clinical translation, that has been largely overlooked in the West.

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“…Isolation and characterization of the activity determined that FGF8 contributed to some of the growth effects. In humans, it is present in increased concentrations in breast cancer cells (Zammit et al, 2002), and its 22-protein family has a wide range of effects from wound healing repair (Clarke et al, 1993;Cuevas, 1998) to tumeriogenesis (Davies et al, 1996). In cattle, there are no reports linking FGF8 to variations in carcass quality.…”

Section: Qtl Analysismentioning

confidence: 99%

Polymorphisms in positional candidate genes on BTA14 and BTA26 affect carcass quality in beef cattle

Marques

Nkrumah²,

Sherman

et al. 2009

Journal of Animal Science

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Several studies have reported the presence of carcass quality QTL on BTA14 and BTA26, with no specific genes being conclusively linked as their cause. The aim of this study was to identify polymorphisms in genes known to affect lipid metabolism in other species and to assess their association with carcass quality traits. Two genes located on BTA14, 2,4 dienoyl CoA reductase 1 (DECR1) and core binding factor, runt domain, alpha subunit 2, translocated to 1 gene (CBFA2T1), have been previously evaluated in other species and found to contain polymorphisms influencing lipid metabolism. A gene on BTA26, fibroblast growth factor 8 (FGF8), has in recent studies been linked to several QTL affecting obesity in mice, indicating its potential for regulating adiposity in other species. Sequencing analysis identified 9 polymorphisms in DECR1, 4 in CBFA2T1, and 4 in FGF8. Multiple sequence alignment of DECR1 among cattle, humans, and mice showed that 4 of these mutations lie in conserved regions across these species. Using 464 Angus, Charolais, and crossbred animals produced associations with ultrasound marbling score (CBFA2T1, P = 0.019), ultrasound backfat (DECR1, P = 0.012), carcass backfat (FGF8, P = 0.004), and lean meat yield (FGF8, P = 0.005). Quantitative trait loci analysis including a set of previously genotyped markers on BTA14, and 1 DECR1 polymorphism resulted in several significant QTL peaks: ultrasound backfat (UBF) at 91 cM, lean meat yield at 86 cM, carcass gradefat at 15 cM, and yield grade at 87 cM, all at the P < 0.05 level. Using DECR1 as a genetic covariate removed the UBF QTL, indicating that this SNP was contributing to the variation observed in UBF. A similar analysis was performed on BTA26 using 1 of the FGF8 polymorphisms. Results showed significant peaks for lean meat yield at 2 cM and for yield grade at 25 cM, both at P < 0.01, and for carcass backfat at 25 cM (P < 0.05). Removal of FGF8 SNP in further analysis resulted in the disappearance of the carcass backfat QTL. These results suggest that polymorphisms discovered in DECR1, CBFA2T1, and FGF8 may play a role in the lipid metabolism pathway affecting carcass quality traits in beef cattle. However, further studies are needed to confirm that these polymorphisms are responsible for the differences observed in carcass quality in beef cattle.

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Effect on tumorigenicity and metastasis of transfection of a diploid benign rat mammary epithelial cell line with DNA corresponding to the mRNA for basic fibroblast growth factor

Cited by 9 publications

References 15 publications

Peer Review #2 of "Fibroblast growth factors as tissue repair and regeneration therapeutics (v0.1)"

Peer Review #2 of "Fibroblast growth factors as tissue repair and regeneration therapeutics (v0.1)"

Fibroblast growth factors as tissue repair and regeneration therapeutics

Polymorphisms in positional candidate genes on BTA14 and BTA26 affect carcass quality in beef cattle

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