Effection of cordycepin on Inhibition proliferation by activating autophagy in HepG2 cell

Abstract

Cordycepin, the main active component of Cordyceps militaris, possesses a variety of biological activities. This paper investigated the inhibition of cordycepin on liver tumors. HepG2 cells were treated with cordycepin. The inhibition rates of cell proliferation was detected by MTT, LC3 protein was detected by immunofluorescence method, autophagy was observed by transmission electron microscopy, autophagy related protein was detected by Western blot. The results showed that cordycepin significantly inhibited the proliferation of HepG2 cells, and significantly increased the expression level of LC3 protein (<0.05). and the autophagy increased in the cells. The Atg-related protein analysis showed that cordycepin significantly reduced the p-mTOR / β-actin ratio and p62 / β-actin ratio (<0.01), and significantly increased the LC3II/LC3I ratio (P<0.01). The results suggested that cordycepin inhibited the proliferation by activating autophagy in HepG2 cells.

The effect of cordycepin on brain oxidative stress and protein expression in streptozotocin-induced diabetic mice

“…Finally, autophagosomes are linked by adaptor proteins such as P62 to deliver aggregated proteins to lysosomes for degradation [ 28 ]. Normally, the autophagy is at a low level under physiological conditions [ 68 ]. In disease conditions especially in DM, a large number of abnormally folded proteins, impaired mitochondria, and free radicals caused to proteins and organelle damaged and produce toxic effects on cell [ 6 ].…”

“…Therefore, activation of autophagy is an acute response of damaged cells and helps to protecting cell [ 75 ]. There have many studies found that cordycepin could ameliorated the disease, especially in DM complications, via inducing autophagy both of in vitro and in vivo [ 10 , 22 , 43 , 68 ]. As previous described, cordycepin could inducing autophagy via AMPK phosphorylation [ 18 , 74 ].…”