Effective amino acid composition of seaweeds inducing food preference behaviors in Aplysia kurodai

Nagahama, Tatsumi; Fujimoto, Kiyo; Takami, Shigemi; Kinugawa, Aiko; Narusuye, Kenji

doi:10.1016/j.neures.2009.03.007

Cited by 8 publications

(2 citation statements)

References 37 publications

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“…In particular, an East-Asian marine gastropod, the sea hare ( Aplysia kurodai ), shows a clear preference for sea lettuce, feeding well on the lettuce but rejecting Gelidium and Pachydictyon [12]. Investigation of sea hare digestion may therefore provide useful clues for establishment of an artificial process for saccharifying polysaccharides in sea lettuce.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Enzymatic Analysis of the Cellulolytic System in Digestive Fluid of the Sea Hare Aplysia kurodai. Efficient Glucose Release from Sea Lettuce by Synergistic Action of 45 kDa Endoglucanase and 210 kDa ß-Glucosidase

et al. 2013

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Although many endo-ß-1,4-glucanases have been isolated in invertebrates, their cellulolytic systems are not fully understood. In particular, gastropod feeding on seaweed is considered an excellent model system for production of bioethanol and renewable bioenergy from third-generation feedstocks (microalgae and seaweeds). In this study, enzymes involved in the conversion of cellulose and other polysaccharides to glucose in digestive fluids of the sea hare (Aplysia kurodai) were screened and characterized to determine how the sea hare obtains glucose from sea lettuce (Ulva pertusa). Four endo-ß-1,4-glucanases (21K, 45K, 65K, and 95K cellulase) and 2 ß-glucosidases (110K and 210K) were purified to a homogeneous state, and the synergistic action of these enzymes during cellulose digestion was analyzed. All cellulases exhibited cellulase and lichenase activities and showed distinct cleavage specificities against cellooligosaccharides and filter paper. Filter paper was digested to cellobiose, cellotriose, and cellotetraose by 21K cellulase, whereas 45K and 65K enzymes hydrolyzed the filter paper to cellobiose and glucose. 210K ß-glucosidase showed unique substrate specificity against synthetic and natural substrates, and 4-methylumbelliferyl (4MU)-ß-glucoside, 4MU–ß-galactoside, cello-oligosaccharides, laminarin, and lichenan were suitable substrates. Furthermore, 210K ß-glucosidase possesses lactase activity. Although ß-glucosidase and cellulase are necessary for efficient hydrolysis of carboxymethylcellulose to glucose, laminarin is hydrolyzed to glucose only by 210K ß-glucosidase. Kinetic analysis of the inhibition of 210K ß-glucosidase by D-glucono-1,5-lactone suggested the presence of 2 active sites similar to those of mammalian lactase-phlorizin hydrolase. Saccharification of sea lettuce was considerably stimulated by the synergistic action of 45K cellulase and 210K ß-glucosidase. Our results indicate that 45K cellulase and 210K ß-glucosidase are the core components of the sea hare digestive system for efficient production of glucose from sea lettuce. These findings contribute important new insights into the development of biofuel processing biotechnologies from seaweed.

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Section: Introductionmentioning

confidence: 99%

Comprehensive Enzymatic Analysis of the Cellulolytic System in Digestive Fluid of the Sea Hare Aplysia kurodai. Efficient Glucose Release from Sea Lettuce by Synergistic Action of 45 kDa Endoglucanase and 210 kDa ß-Glucosidase

et al. 2013

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“…Commercial extracts made from brown algae (Ascophyllum nodosum) contain a wide range of inorganic and organic components (Verkleij, 1992;Sharma et al, 2014) including nitrogen, phosphorus, potassium, calcium, iron, magnesium, zinc, sodium and sulfur (Fleurence, 2004;Rayirath et al, 2009). Brown algae extracts also contain varying amounts of amino acids (Fleurence, 2004;Rayirath et al, 2009;Blunden et al, 2010;MacKinnon et al, 2010;Gutiérrez-Gamboa et al, 2020c) and only between 3 to 15% of their dry weight in proteins, representing a lower content than that found in green and red algae, which varies from 10 to 47% of their dry weight (Fleurence, 1999(Fleurence, , 2004Nagahama et al, 2009;Sharma et al, 2014;Gutiérrez-Gamboa, 2020).…”

Section: Species Of Algae Used As Extracts and Their Compositionmentioning

confidence: 99%

Seaweeds in viticulture: a review focused on grape quality

Gutiérrez‐Gamboa

Moreno-Simunovic

2021

Ciência Téc. Vitiv.

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Cell walls of seaweeds contain a wide number of organic and inorganic constituents, of which polysaccharides have important biological activity. Some researchers suggest that polysaccharides from seaweeds can behave as biotic elicitors in viticulture, triggering the synthesis of phenolic compounds in leaves and grape berries. The mechanism of action of seaweeds after a foliar application to grapevines is not fully understood but it is discussed in this review. An overview of the recent research focused on the effects of seaweeds foliar applications on grapevine productivity, on grape and wine quality is included as well as a short-term future perspective for the research in this field.

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Effects of aging on the food intake in the feeding behavior of Aplysia kurodai

et al. 2016

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In wild Aplysia, the birthdate of animals can typically not be determined. Therefore, we sought a reliable index of old age by taking into consideration the distinguished Japanese seasons. Large amounts of eggs and dead bodies were present on the coast during and after the second half of May (MayS). Body mass decreased after May. We roughly classified animals collected before and after the MayS as mature and old animals. Plots of internalized shell length (S) against body mass (B) gave distinct best-fit curves for mature and old animals. The B/S significantly decreased in the second half of June, suggesting that body mass decreases with age but shell length is maintained in each animal. Therefore, the collected animals were classified into mature and old animals using the best-fit curves for animals classified by the collection period. We examined the amount of food intake every 2 h up to 8 h after providing food. The amounts increased linearly, and the rate was significantly lower in old animals than in mature animals. The amount of 1-day food intake was also significantly lower in old animals. These results suggest that food intake may decline with age and this may cause mass loss in old animals.

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Effective amino acid composition of seaweeds inducing food preference behaviors in Aplysia kurodai

Cited by 8 publications

References 37 publications

Comprehensive Enzymatic Analysis of the Cellulolytic System in Digestive Fluid of the Sea Hare Aplysia kurodai. Efficient Glucose Release from Sea Lettuce by Synergistic Action of 45 kDa Endoglucanase and 210 kDa ß-Glucosidase

Comprehensive Enzymatic Analysis of the Cellulolytic System in Digestive Fluid of the Sea Hare Aplysia kurodai. Efficient Glucose Release from Sea Lettuce by Synergistic Action of 45 kDa Endoglucanase and 210 kDa ß-Glucosidase

Seaweeds in viticulture: a review focused on grape quality

Effects of aging on the food intake in the feeding behavior of Aplysia kurodai

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