Effective and complex stimulation of the biodegradation system of fungus Cerrena unicolor by rapeseed meal fermentation

Abstract

The effect of supplementation of medium with rapeseed meal (RM) on production of biotechnologically important enzymes was investigated in submerged cultures of the white rot fungus Cerrena unicolor. The addition of RM (3.5% w/v) distinctly stimulated the activities of laccase, chitinase, and β-glucosidase. As compared to the control, the activities of chitinase, β-glucosidase, and laccase in the RM supplemented cultures were up to 4.1, 8.4, and 3.9 times higher, respectively. The results of the spectrophotometric and spectrofluorometric measurements were additionally confirmed by zymographic analysis of the samples. The level of sugars and phenolic compounds as well as the antioxidative ability of fungal preparations were also determined. The results obtained indicate that the submerged liquid fermentation of rapeseed meal can be proposed as an inexpensive and very effective method for biotechnological production of chitinase, β-glucosidase, and laccase by C. unicolor.

Lignocellulose resources for the Myrothecium roridum laccase production and their integrated application for dyes removal

“…The inductive effect of GA, HBA, CA, FA, p-coumaric, and sinapic acid on laccase has been reported earlier (Wang et al 2014;Adekunle et al 2017). The effect of supplementation of medium with RPC on the production of laccase, chitinase, and β-glucosidase by white rot fungus Cerrena unicolor has been previously described by Jaszek et al (2016). The addition of RPC (3.5% w/v) distinctly stimulated the activities of enzymes.…”

“…They require only air as the co-substrate and release water as the by-product; therefore, they can be classified as green catalysts (Mate and Alcalde 2017). Laccases from Bacillus subtilis, B. licheniformis, Trametes trogii, Pleurotus ostreatus, Pycnoporus sangineus, Ganoderma lucidum, Cerrena unicolor have been suggested for the effective dye degradation (Kuhar et al 2015;Campos et al 2016;Jaszek et al 2016;Zimbardi et al 2016;Qiao et al 2017;Wang et al 2017;Bhavsar et al 2018;Zhuo et al 2018). However, enzymatic biodegradation has to meet several criteria in order for it to be profitable, effective, and environment-friendly.…”

The unconventional adverse effects of fungal pretreatment on iturin A fermentation by Bacillus amyloliquefaciens CX‐20

“…RSM is a good protein source for the microbial fermentation of high‐value‐added products (Wang et al ., 2010; Kiran et al ., 2013; Chatzifragkou et al ., 2014; Jaszek et al ., 2016; Prendecka et al ., 2016; Boratyński et al ., 2018), but its particular physical and chemical structure limited the nutrients to be directly assimilated by the majority of industrial microorganisms without pretreatment (Wang et al ., 2010). Consequently, the application of RSM hydrolysate produced following fungal pretreatment as a feedstock for microbial bioconversions has been intensively investigated (Wang et al ., 2010; Kiran et al, 2012, 2013; García et al ., 2013; Chatzifragkou et al ., 2014; Salakkam et al ., 2017; Salakkam and Webb, 2018).…”

“…In recent years, RSM has been widely used as a low‐cost raw material in fermentation processes for the industrial production of various value‐added microbial metabolites (Kiran et al, 2012, 2013; Chatzifragkou et al ., 2014; Almeida et al ., 2015; Jaszek et al ., 2016; Prendecka et al ., 2016). RSM can not only be used as a nitrogen source (Kiran et al ., 2012; García et al ., 2013; Chatzifragkou et al ., 2014; Jin et al ., 2015), but also provide carbon for the growth and metabolism of microorganisms (Chen et al ., 2011; Almeida et al ., 2015).…”

Genomic Studies of White-Rot Fungus Cerrena unicolor SP02 Provide Insights into Food Safety Value-Added Utilization of Non-Food Lignocellulosic Biomass

“…Lac of SP02 in RS increased substantially in the late period show off-growth condition may cause stimulation of metabolite production to produce higher amounts of secondary metabolites under suboptimal conditions. It happened occasionally that adverse conditions to a white-rot fungal cell instead have positive effects on lac expression, which can be attributed to the strategies possessed by the organisms for their survival under extreme environments [103][104][105]. Based on this, we speculate inhibiting compounds and protective factors exist and antagonization in RS/PB cultivation, which forced SP02 to change the metabolic activity of lignocellulosic secretases to maintain steady growth.…”