Effective bacterial insecticidal proteins against coleopteran pests: A review

Abstract

Coleoptera, the order of insects commonly referred to as beetles, are able to survive in various environments, and thus, comprise the largest order in the animal kingdom. Coleopterans mainly include coprophagous and phytophagous lineages, and many species of the latter lineage are serious pests. In addition to traditional chemical methods, biocontrol measures using various bacterial insecticidal proteins have also gradually been developed to control these insect pests. In this review, we summarized the possible coleopteran‐pest‐specific bacteria and insecticidal proteins that have been reported in the literature thus far and have provided a comprehensive overview and long‐term guidance for the control of coleopteran pests in the future.

Characterization of Two Novel Bacillus thuringiensis Cry8 Toxins Reveal Differential Specificity of Protoxins or Activated Toxins against Chrysomeloidea Coleopteran Superfamily

“…The phylogenetic analysis of Cry8Ha toxin core sequence showed that this proteins is more related to Cry3 proteins and the analysis of the different domains of Cry8Ha1 and Cry8Ia1 proteins confirmed that Cry8Ha1 is more related to the coleopteran active proteins Cry3, Cry1B, and Cry1I that have been shown to be specifically toxic to Chrysomeloidea insects [ 5 ]. Our data suggest that the novel Cry8Ha1 and Cry8Ia1 proteins have evolved from different origins, since these Cry8Ha1 and Cry8Ia1 proteins do not cluster together.…”

“…However, comparing to the beetle’s diversity, the currently discovered variability of Bt insecticidal proteins effective against coleopteran insects is scarce. Currently, only few members in Cry1, Cry3, Cry6 (now APP6), Cry7 Cry8, Cry22 (now XPP22), Cry23/Cry37 (now MPP23/XPP37), Cry34/Cry35 (now GPP34/TPP35), Cry55 (now XPP55), Cyt1, Cyt2, Sip (now MPP5), and Vip1/Vip2 subgroups of Bt protein families were reported to be toxic against certain coleopteran insects [ 5 , 6 ]. Hence, screening of Bt strains and identification of novel insecticidal proteins active against coleopteran insects, especially proteins that display a broad insecticidal spectrum against Chrysomeloidea and Scarabaeoidea is still needed for the effective control of these pests.…”

Circabidian rhythm and its mechanism in <i>Holotrichia parallla</i>

“…オオクロコガネ Holotrichia parallelaはコウチュウ目コ ガネムシ科コフキコガネ亜科コフキコガネ族クロコガネ亜 族クロコガネ Holotrichia 属に分類される 23,17) ，体長２cm ほどの大きなコウチュウの１種である。名前の通り黒いコ ガネムシだが，日本にいる Holotrichia convexopyga マル オクロコガネやHolotrichia kiotonensis クロコガネが艶 のある黒色であるのに対し，本種はマット感のある黒を呈 している。Holotrichiaは大きなグループであり，東南ア ジア，東アジアに広く生息する 1) 。幼虫が地中で農作物を 食 害 す る た め， イ ン ド で はHolotrichia serrataや Holotrichia consanguineaが落花生の大害虫として知ら れており，日本ではオオクロコガネはサツマイモやゴルフ 場 の 芝 生 の 根， リ ュ ウ キ ュ ウ ク ロ コ ガ ネ Holotrichia loochooana loochooanaはサトウキビの害虫として知られ ている。これらの種について，駆除のための昆虫寄生ウィ ルスや化学生態，嗅覚系の研究がおこなわれてきた 2,5,14,32) 。 これまでにオオクロコガネが２日毎に地上へ出現するこ とや 34) ，メスのフェロモン腺が２日毎に大きくなる 19…”

Chromobacterium Csp_P biopesticide is toxic to larvae of three Diabrotica species including strains resistant to Bacillus thuringiensis

“…Bacterial insecticidal proteins have been used against insect pests for many years, especially lepidopteran, coleopteran, and dipteran pests, with Bt being the most widely used 17 , 18 . New insecticidal proteins produced by soil bacteria other than Bt such as Photorhabdus luminescens 19 , Chromobacterium piscinae 20 , 21 , Pseudomonas chlororaphis and P. mosselii 22 – 25 , Alkaligenes faecalis 26 , 27 and Brevibacillus laterosporus 26 , 28 , and even those produced by mushrooms from the genus Pleurotus 29 have been explored as future venues for corn rootworm management and resistance management 18 .…”