Effective Bone Regeneration Using Thermosensitive Poly(<i>N</i>-Isopropylacrylamide) Grafted Gelatin as Injectable Carrier for Bone Mesenchymal Stem Cells

Ren, Zhiwei; Wang, Yang; Ma, Shiqing; Duan, Shun; Yang, Xiaoping; Gao, Ping; Zhang, Xu; Cai, Qing

doi:10.1021/acsami.5b02821

Cited by 93 publications

(79 citation statements)

References 41 publications

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“…PNIPAAM is a synthetic polymer that can be tuned to match the mechanical stiffness of bone once injected at body temperature [157–159]. Watson et al demonstrated that decorating PNIPAAM with pendant phosphate groups improved biodegradation and biointegration of the hydrogel with host tissue, as well as support enhanced mineralization, MSC differentiation, and bone formation in a rat cranial defect model [159].…”

Section: Specific Hydrogel Design Choices For Specific Tissue Applmentioning

confidence: 99%

Design of Injectable Materials to Improve Stem Cell Transplantation

2016

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Stem cell-based therapies are steadily gaining traction for regenerative medicine approaches to treating disease and injury throughout the body. While a significant body of work has shown success in preclinical studies, results often fail to translate in clinical settings. One potential cause is the massive transplanted cell death that occurs post injection, preventing functional integration with host tissue. Therefore, current research is focusing on developing injectable hydrogel materials to protect cells during delivery and to stimulate endogenous regeneration through interactions of transplanted cells and host tissue. This review explores the design of targeted injectable hydrogel systems for improving the therapeutic potential of stem cells across a variety of tissue engineering applications with a focus on hydrogel materials that have progressed to the stage of preclinical testing.

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Section: Specific Hydrogel Design Choices For Specific Tissue Applmentioning

confidence: 99%

Design of Injectable Materials to Improve Stem Cell Transplantation

2016

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“…79 Poly( N -isopropylacrylamide) (PNIPAAm) and Pluronic ® are typical examples of thermosensitive polymers that have been used for bone cells’ encapsulations. 80,81 …”

Section: Hydrogels: Suitable Micro-environments For Btementioning

confidence: 99%

Cellularizing hydrogel-based scaffolds to repair bone tissue: How to create a physiologically relevant micro-environment?

et al. 2017

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Tissue engineering is a promising alternative to autografts or allografts for the regeneration of large bone defects. Cell-free biomaterials with different degrees of sophistication can be used for several therapeutic indications, to stimulate bone repair by the host tissue. However, when osteoprogenitors are not available in the damaged tissue, exogenous cells with an osteoblast differentiation potential must be provided. These cells should have the capacity to colonize the defect and to participate in the building of new bone tissue. To achieve this goal, cells must survive, remain in the defect site, eventually proliferate, and differentiate into mature osteoblasts. A critical issue for these engrafted cells is to be fed by oxygen and nutrients: the transient absence of a vascular network upon implantation is a major challenge for cells to survive in the site of implantation, and different strategies can be followed to promote cell survival under poor oxygen and nutrient supply and to promote rapid vascularization of the defect area. These strategies involve the use of scaffolds designed to create the appropriate micro-environment for cells to survive, proliferate, and differentiate in vitro and in vivo. Hydrogels are an eclectic class of materials that can be easily cellularized and provide effective, minimally invasive approaches to fill bone defects and favor bone tissue regeneration. Furthermore, by playing on their composition and processing, it is possible to obtain biocompatible systems with adequate chemical, biological, and mechanical properties. However, only a good combination of scaffold and cells, possibly with the aid of incorporated growth factors, can lead to successful results in bone regeneration. This review presents the strategies used to design cellularized hydrogel-based systems for bone regeneration, identifying the key parameters of the many different micro-environments created within hydrogels.

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“…The physical properties of hydrogels can be easily manipulated: thermoresponsive solutions that gelatinize at physiologic temperature can be premixed with either cells or growth factors prior to implantation (26). Alternatively, Han et al have developed a hydrogel formulation consisting of microribbons with variable crosslinking ability, thus allowing for specification of pore size based on desired application.…”

Section: Translational Cell-based Studiesmentioning

confidence: 99%

Stem Cells in Bone Regeneration

Walmsley

Ransom

Zielins

et al. 2016

Stem Cell Rev and Rep

131

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Bone has the capacity to regenerate and repair itself. However, this capacity may be impaired or lost depending on the size of the defect or the presence of certain disease states. In this review, we discuss the key principles underlying bone healing, efforts to characterize bone stem and progenitor cell populations, and the current status of translational and clinical studies in cell-based bone tissue engineering. Though barriers to clinical implementation still exist, the application of stem and progenitor cell populations to bone engineering strategies has the potential to profoundly impact regenerative medicine.

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Effective Bone Regeneration Using Thermosensitive Poly(N-Isopropylacrylamide) Grafted Gelatin as Injectable Carrier for Bone Mesenchymal Stem Cells

Cited by 93 publications

References 41 publications

Design of Injectable Materials to Improve Stem Cell Transplantation

Design of Injectable Materials to Improve Stem Cell Transplantation

Cellularizing hydrogel-based scaffolds to repair bone tissue: How to create a physiologically relevant micro-environment?

Stem Cells in Bone Regeneration

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