Effective control of dental chair unit waterline biofilm and marked reduction of bacterial contamination of output water using two peroxide-based disinfectants

Tuttlebee, C.M.; O’Donnell, Mary; Keane, C.; Russell, R.J.; Sullivan, Derek J.; Falkiner, F.R.; Coleman, David C.

doi:10.1053/jhin.2002.1282

Cited by 79 publications

(147 citation statements)

References 32 publications

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“…Tuttlebee et al (43) compared Sanosil (5 to 8 weeks) and Sterilex Ultra (7 to 20 weeks) in GDPs using once-weekly disinfection of the DUWS, and both products reduced TVCs significantly to below the ADA guideline of Յ200 CFU · ml Ϫ1 . Using microscopy imaging techniques, a significant reduction in biofilm coverage has also been observed (43,48). Similar results were reported for both products in laboratory models of DUWS (22,48).…”

Section: Discussionsupporting

confidence: 80%

“…Unfortunately, the application of Sterilex Ultra was stopped in three DUWS due to material incompatibility issues. The problem of blocking of DUWS tubing when using Sterilex Ultra has been described before (43) and may be caused by sedimentation of excess Sterilex Ultra that has not dissolved settling onto the tubing's narrow inner surface. In a previous (16).…”

Section: Discussionmentioning

confidence: 99%

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Comparison of the Efficacies of Disinfectants To Control Microbial Contamination in Dental Unit Water Systems in General Dental Practices across the European Union

Schel

Marsh

Bradshaw³

et al. 2006

Appl Environ Microbiol

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Water delivered by dental unit water systems (DUWS) in general dental practices can harbor high numbers of bacteria, including opportunistic pathogens. Biofilms on tubing within DUWS provide a reservoir for microorganisms and should be controlled. This study compared disinfection products for their ability to meet the American Dental Association's guideline of <200 CFU · ml ؊1 for DUWS water. Alpron, BioBlue, Dentosept, Oxygenal, Sanosil, Sterilex Ultra, and Ster4Spray were tested in DUWS (n ‫؍‬ 134) in Denmark, Germany, Greece, Ireland, The Netherlands, Spain, and the United Kingdom. Weekly water samples were tested for total viable counts (TVCs) on yeast extract agar, and, where possible, the effects of products on established biofilm (TVCs) were measured. A 4-to 5-week baseline measurement period was followed by 6 to 8 weeks of disinfection (intermittent or continuous product application). DUWS water TVCs before disinfection ranged from 0 to 5.41 log CFU · ml ؊1 . Disinfectants achieved reductions in the median water TVC ranging from 0.69 (Ster4Spray) to 3.11 (Dentosept) log CFU · ml ؊1 , although occasional high values (up to 4.88 log CFU · ml ؊1 ) occurred with all products. Before treatment, 64% of all baseline samples exceeded American Dental Association guidelines, compared to only 17% following commencement of treatment; where tested, biofilm TVCs were reduced to below detectable levels. The antimicrobial efficacies of products varied (e.g., 91% of water samples from DUWS treated with Dentosept or Oxygenal met American Dental Association guidelines, compared to 60% of those treated with Ster4Spray). Overall, the continuously applied products performed better than those applied intermittently. The most effective products were Dentosept and Oxygenal, although Dentosept gave the most consistent and sustained antimicrobial effect over time.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Comparison of the Efficacies of Disinfectants To Control Microbial Contamination in Dental Unit Water Systems in General Dental Practices across the European Union

Schel

Marsh

Bradshaw³

et al. 2006

Appl Environ Microbiol

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“…The effectiveness of biocides is tested under laboratory conditions as well as in dental offi ce (12,13). Tuttlebee and his team tested two peroxide-based biocides and found that Sanosil was more suitable for repeated DUWL decontamination (14). A multicenter study held in seven EU countries yielded highly interesting results concerning the microbial contamination of DUWL water.…”

Section: Introductionmentioning

confidence: 99%

Legionella spp. in dental unit waterlines

Jurásková¹,

Sedlackova²,

Janska³

et al. 2017

BLL

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OBJECTIVE: To determine the current presence of Legionella spp. in the output water of dental unit waterlines (DUWLs) and examine its mitigation by disinfection at the Institute of Dentistry and Oral Sciences, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc. MATERIAL AND METHODS: The fi rst stage of our survey involved collecting samples of DUWL output water from 50 dental chair units (DCUs), and 2 samples of the incoming potable water. In October 2015, a one-time disinfection (1 % Stabimed) of DUWLs was conducted. This was followed by collecting 10 control samples (survey stage 2). RESULTS: From the total of 50 samples (survey stage 1), 18 samples (36.0 %) tested positive for Legionella spp. Following the disinfection, nine of the ten samples no longer showed any presence of Legionella. CONCLUSION: Based on culture results, the one-time disinfection (1 % Stabimed) was effective. We are unable to comment on the duration of positive effect of disinfection on the occurrence of Legionella spp. in the outlet water. It was a one-time survey (Tab. 2, Ref. 32). Text in PDF www.elis.sk. KEY WORDS: Legionella spp., water of dental unit waterlines, disinfection.

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“…15 Dental ünitlerin su borularının dezenfeksiyonunda; hidrojen peroksit, 16 perasetik asit, 17 ozon, 18 sodyum hipoklorit, 15 superokside edilmiĢ su 19 gibi solüsyonlar kullanılmaktadır.…”

Section: (Tablo 1)unclassified