Effective control of Shigella contamination in different foods using a novel six-phage cocktail

Shahin, Khashayar; Zhang, Lili; Delfan, Abbas Soleimani; Komijani, Majid; Hedayatkhah, Abolghasem; Bao, Hongduo; Barazandeh, Mohadeseh; Mansoorianfar, Mojtaba; Pang, Maoda; He, Tao; Bouzari, Majid; Wang, Ran

doi:10.1016/j.lwt.2021.111137

Cited by 17 publications

(4 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, we used tomato (solid; smooth), lettuce (solid; rough), milk (liquid), and chicken skin (solid; complex). Different foods contain different structures, chemical compositions, and nutrition which could affect the phage adsorption process to the host pathogenic bacteria [ 46 ]. In this research, the multiplicity of infection (MOI) between the phage and bacteria host cell was established at 100, where the adsorption of phage was allowed to continue to completion, also phage can adsorb randomly to susceptible cells [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Application of BI-EHEC and BI-EPEC bacteriophages to control enterohemorrhagic and enteropathogenic escherichia coli on various food surfaces

Waturangi

2023

BMC Res Notes

View full text Add to dashboard Cite

Objectives The purposes of this study were to determine the Efficiency of Plating (EOP) value of Bacteriophage BI-EHEC and BI-EPEC and to evaluate the application of these bacteriophages in reducing population of EHEC and EPEC on various food samples. Results In this study, we used bacteriophage BI-EHEC and BI-EPEC, which were isolated from previous study. Both phages were tested with other multiple pathotypes of intestinal pathogenic E. coli to determine the efficiency of plating. BI-EHEC had high efficiency toward ETEC with an EOP value of 2.95 but low efficiency toward EHEC with an EOP value of 0.10, while BI-EPEC had high efficiency toward EHEC and ETEC with EOP values of 1.10 and 1.21, respectively. As biocontrol agents, both bacteriophages able to reduce CFU of EHEC and EPEC in several food samples using 1 and 6-days incubation times at 4 $$\text{?}$$. BI-EHEC reduced the number of EHEC with an overall percentage of bacterial reduction value above 0.13 log10, while BI-EPEC reduced number of EPEC with reduction value above 0.33 log10.

show abstract

Section: Discussionmentioning

confidence: 99%

Application of BI-EHEC and BI-EPEC bacteriophages to control enterohemorrhagic and enteropathogenic escherichia coli on various food surfaces

Waturangi

2023

BMC Res Notes

View full text Add to dashboard Cite

show abstract

“…This is not the case with the use of chemicals, and as the dose is reduced, treatment responses decline dramatically. This is due to the so-called "auto dosing" properties of phages [8][9][10]. Another advantage is their narrow spectrum or host specificity of phages, each infecting a particular range of bacterial species.…”

Section: Introductionmentioning

confidence: 99%

“…Another advantage is their narrow spectrum or host specificity of phages, each infecting a particular range of bacterial species. The phage character does not alter the normal flora of the body, maintaining health conditions [9,10]. Loss of normal flora in the body leads to a decrease in some vitamins and impaired intestinal functions.…”

Section: Introductionmentioning

confidence: 99%

“…Despite the benefits mentioned, some challenges remain such as phage resistance, side effects, positive and negative phage convergence, and the possibility of virulent phage mutation and alteration to moderate (lysogenic) phage for efficient combating against pathogens [13][14][15]. Noticeably, the antibacterial effects of various phages environmental from environmental sources have been delineated against several bacterial pathogens such as Klebsiella pneumonia, Acinetobacter baumannii, Staphylococcus aureus, E. coli, and Shigella species giving admissible results [6][7][8][9][10][11][12][13][14][15]. The aim of the current survey was to assess the therapeutic effect of phages cocktail and single form obtained from hospital sewage in Southern Iran against S. sonnei ATCC® 9290 and S. flexneri ATCC 12022 standard strains.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Antibacterial Effects of Cocktail and Single Forms of Lytic Phages Belonging to Podoviridae and Myoviridae Families from Sewage against Shigella sonnei and Shigella flexneri

Jokar

Rahimian

Ghasemian

et al. 2022

BioMed Research International

View full text Add to dashboard Cite

Background. Diarrhea caused by bacterial pathogens such as Shigella spp. is one of the prominent public health concerns. The evolution of vast antibiotic resistance by these pathogens, leading to failure in the infections eradication, has made an impetus to seek and develop novel approaches. Recently, some alternative therapies such as phage therapy have been investigated. Bacteriophages are viruses that target specific bacterial species. The objective of this study was to assess the therapeutic effect of phages obtained from hospital sewage against Shigella sonnei (S. sonnei) ATCC® 9290 and S. flexneri ATCC 12022 standard and clinical strains. Methods. Four various lytic bacteriophages were isolated from animal fecal and sewage samples and propagated using S. sonnei and S. flexneri as host organisms. The phages’ morphology was determined using transmission electron microscopy (TEM). The lytic potential and host specificity of isolated phages were evaluated using double layer plaque assay and spot test. Moreover, bacterial turbidity values were evaluated in coculture with phages in the Luria Bertani (LB) medium for 24 hours at time intervals of 30 min. Results. Phage cocktails (Shs1, Shs2, Shf1, and Shf2) exhibited higher antimicrobial activity than single phage application against S. sonnei and S. flexneri standard strains. The phages belonged to Podoviridae and Myoviridae families according to TEM-assisted morphological features analysis. In addition, the phages exhibited host specificity using the spot test against 18 Shigella clinical isolates. Conclusion. In this study, phage cocktail of Podoviridae and Myoviridae families from sewage conferred substantial antibacterial effects against S. sonnei and S. flexneri. However, single phage effects were unstable in the LB coculture. Moreover, the phages had host specificity using the spot test performed against Shigella spp. clinical isolates.

show abstract

Isolation, characterization, and genome analysis of a broad host range Salmonella phage vB_SenS_TUMS_E4: a candidate bacteriophage for biocontrol

et al. 2023

View full text Add to dashboard Cite

Effective control of Shigella contamination in different foods using a novel six-phage cocktail

Cited by 17 publications

References 35 publications

Application of BI-EHEC and BI-EPEC bacteriophages to control enterohemorrhagic and enteropathogenic escherichia coli on various food surfaces

Application of BI-EHEC and BI-EPEC bacteriophages to control enterohemorrhagic and enteropathogenic escherichia coli on various food surfaces

The Antibacterial Effects of Cocktail and Single Forms of Lytic Phages Belonging to Podoviridae and Myoviridae Families from Sewage against Shigella sonnei and Shigella flexneri

Isolation, characterization, and genome analysis of a broad host range Salmonella phage vB_SenS_TUMS_E4: a candidate bacteriophage for biocontrol

Contact Info

Product

Resources

About