EFFECTIVE COUNTER-CURRENT CHROMATOGRAPHIC METHOD FOR ONE-STEP PREPARATIVE ISOLATION AND PURIFICATION OF ANTHRAGLYCOSIDE B FROM Begonia fimbristipula USING ELUTION-EXTRUSION SEPARATION MODE

Abstract

& Counter-current chromatography (CCC) is a chromatographic technique with a support-free liquid stationary phase. Elution-extrusion CCC (EECCC) takes full advantage of the liquid nature of the stationary phase and effectively extends the hydrophobicity window with any biphasic liquid system. In this study, a preparative EECCC method for isolation and purification of anthraglycoside B from the ethanol extract of Begonia fimbristipula was successfully established by using n-hexane-ethyl acetate-methanol-water as the two-phase solvent system. The upper phase of n-hexaneethyl acetate-methanol-water (1:6:1:6, v=v) was used as the stationary phase of HSCCC. Under the optimum conditions, 26 mg of anthraglycoside B at 97.8% purity, as determined by UPLC, could be yielded from 500 mg of the crude extract in a single EECCC separation. The peak fraction of CCC was identified by 1 H NMR and 13 C NMR.

Separation of six compounds from pigeon pea leaves by elution–extrusion counter‐current chromatography

“…The phase change indicated the beginning of the extrusion procedure. The strong retention substances were directly pushed out by the stationary phase in the extrusion step . The data were collected with a V4.0 chromatogram workstation (Shanghai Jinda Biochemy Apparatus, Shanghai, China).…”

Elution–extrusion counter‐current chromatographic separation and theoretical mechanism of antioxidant from Robinia pseudoacacia flower

“…The combination of elution and extrusion significantly increased the polarity span of CCC [15,16]. As a novel and efficient separation technology, EECCC has achieved some successful applications [17][18][19][20].…”