Effective delivery of immunosuppressive drug molecules by silica coated iron oxide nanoparticles

Hwang, Jang‐Sun; Lee, Eunwon; Kim, Jieun; Seo, Youngmin; Lee, Kwan Hong; Hong, Jong Wook; Gilad, Assaf A.; Park, Hansoo; Choi, Jonghoon

doi:10.1016/j.colsurfb.2016.01.040

Cited by 43 publications

(28 citation statements)

References 38 publications

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“…A172 astrocytes are not tumorigenic and have wild-type p53 (Wolff et al 1999;Qiang et al 2009;Sato et al 2009). Different surface coatings have been applied to ION to stabilize their highly reactive surface and to provide them with a moiety for anchoring tags and/or drug molecules (Hwang et al 2016). Oleic acid has diverse advantages that make it very appropriate for some biomedical applications, because it stabilizes the nanoparticles and enables the surface for functionalization.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of cytotoxicity and genotoxicity induced by oleic acid‐coated iron oxide nanoparticles in human astrocytes

Fernández-Bertólez

Costa

Brandão

et al. 2019

Environ and Mol Mutagen

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Iron oxide nanoparticles (ION) are gaining importance as diagnostic and therapeutic tool of central nervous system diseases. Although oleic acid‐coated ION (O‐ION) have been described as stable and biocompatible, their potential neurotoxicity was scarcely evaluated in human nervous cells so far. The primary aim of this work was to assess the molecular and cellular effects of O‐ION on human astrocytes (A172 cells) under different experimental conditions. An extensive set of cyto‐ and genotoxicity tests was carried out, including lactate dehydrogenase release assay, cell cycle alterations, and cell death production, as well as comet assay, γH2AX assay, and micronucleus (MN) test, considering also iron ion release capacity and alterations in DNA repair ability. Results showed a moderate cytotoxicity related to cell cycle arrest and cell death promotion, regardless of serum presence. O‐ION induced genotoxic effects, namely primary DNA damage, as detected by the comet assay and H2AX phosphorylation, but A172 cells were able to repair this particular damage because no chromosome alterations were found (confirmed by MN test results). Accordingly, no effects on the DNA repair ability were observed. The presence of serum proteins did not influence O‐ION toxicity. Iron ions released from the O‐ION surface seemed not to be responsible for the cytotoxic and genotoxic effects observed. Environ. Mol. Mutagen. 2019. © 2019 Wiley Periodicals, Inc.

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Section: Introductionmentioning

confidence: 99%

Evaluation of cytotoxicity and genotoxicity induced by oleic acid‐coated iron oxide nanoparticles in human astrocytes

Fernández-Bertólez

Costa

Brandão

et al. 2019

Environ and Mol Mutagen

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“…These analytical techniques have excellent accuracy, precision, and detection limits, but they have a drawback in that their preprocessing takes a long time and the costs for analysis, constructing the equipment, and maintenance are very high. On the other hand, quantitative analysis methods for measuring the content of drugs loaded in nanoparticles relatively easily include instrumental analysis using an ultraviolet-visible (UV/Vis) spectrophotometer [5,6] and a fluorescence spectrophotometer [7,8]. The linearity of the calibration curve for the standard substance is evaluated and defined as the concentration of the analyte in terms of the absorbance and fluorescence intensity along the y-axis and the x-axis as the concentration.…”

Section: Introductionmentioning

confidence: 99%

Quantification of Unknown Nanoscale Biomolecules Using the Average-Weight-Difference Method

et al. 2019

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In order to quantify the amount of drug molecules in drug delivery systems, analytical techniques such as high-performance liquid chromatography are used due to their accuracy and reliability. However, the instruments required for such techniques are expensive and not available in all laboratories. Therefore, in this study, we introduce a method that can be a relatively inexpensive and easy to perform drug analysis in almost any laboratory set-up. We have devised the “average-weight-difference method” within the limits of existing spectral analyses. By employing this method, we quantitatively analyzed the amount of isoniazid or doxorubicin molecules loaded onto β-glucan nanoparticles. This proved to be a relatively simple and reliable method and can be used to estimate the amount of nanoscale biomolecules before their analysis through expensive equipment in an environment where the instruments are not readily available.

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“…Silica-coated iron oxide nanoparticles are now being actively tested for their cytotoxicity [29] and internalization capability [30][31][32]. Data collected until now suggest silica-coated nanoparticles as very promising agents for drug delivery [17,31], MRI [33], and other biomedical applications [33,34]. Yet, although this type of nanoparticle coating is currently being actively studied, there are still no comprehensive data on its biological effects and hazards.…”

Section: Coatingmentioning

confidence: 99%

“…the nanosystems simultaneously used for both therapy and diagnostics [11,16,17]. This is possible mostly on account of the coatings, which during the respective applications will help keep the particles finely dispersed and reduce nonspecific protein adsorption and clearance by macrophages [8].…”

Section: Coatingmentioning

confidence: 99%

Physicochemical Properties of Magnetic Nanoparticles: Implications for Biomedical Applications In Vitro and In Vivo

Belanova¹,

Gavalas

Makarenko

et al. 2018

Oncol Res Treat

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Magnetic and superparamagnetic iron oxide nanoparticles are emerging as promising candidates for various applications in biology and medicine, and especially in oncology. These applications, however, require that a specific set of physical, chemical, and biological properties be combined in a given sample of nanoparticles for them to act as intended. Some of these properties are fundamental: They strictly determine the nanoparticles' behavior both in vitro and in vivo. These properties are the charge, the solution stability and zeta potential, and the coating of the nanoparticles. A certain combination of these properties may satisfy a researcher in an in vitro study, but other properties should also be considered when in vivo applications are planned. For in vivo experiments, additional determinants of the quality of nanoparticles are their size, shape, modifications with targeting moieties, and degradation/excretion pathways. All these properties are in the focus of the present review.

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Effective delivery of immunosuppressive drug molecules by silica coated iron oxide nanoparticles

Cited by 43 publications

References 38 publications

Evaluation of cytotoxicity and genotoxicity induced by oleic acid‐coated iron oxide nanoparticles in human astrocytes

Evaluation of cytotoxicity and genotoxicity induced by oleic acid‐coated iron oxide nanoparticles in human astrocytes

Quantification of Unknown Nanoscale Biomolecules Using the Average-Weight-Difference Method

Physicochemical Properties of Magnetic Nanoparticles: Implications for Biomedical Applications In Vitro and In Vivo

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