Effective Dose of Lactosucrose on Fecal Flora and Fecal Metabolites of Humans

Hara, Hiroyoshi; Li, Shou-Tou; Sasaki, Masakuni; Maruyama, Tsukasa; Terada, Atsushi; Ogata, Yukiko; Fujita, Katsuhide; Ishigami, Hiroshi; Hara, Kozo; Fujimori, Isao; Mitsuoka, Tomotari

doi:10.12938/bifidus1982.13.2_8

Cited by 64 publications

(40 citation statements)

References 26 publications

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“…Lactosucrose is produced from a mixture of lactose and sucrose using the enzyme ␤-fructofuranosidase (Playne & Crittenden, 1996). The fructosyl residue is transferred from sucrose to the C 1 position of the glucose moiety in the lactose, producing a non-reducing oligosaccharide (Hara et al 1994).…”

Section: Lactosucrosementioning

confidence: 99%

Dietary modulation of the human colonic microbiota: updating the concept of prebiotics

Gibson

Probert

Loo³

et al. 2004

Nutr. Res. Rev.

2,485

1,777

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Prebiotics are non-digestible (by the host) food ingredients that have a beneficial effect through their selective metabolism in the intestinal tract. Key to this is the specificity of microbial changes. The present paper reviews the concept in terms of three criteria: (a) resistance to gastric acidity, hydrolysis by mammalian enzymes and gastrointestinal absorption; (b) fermentation by intestinal microflora; (c) selective stimulation of the growth and/or activity of intestinal bacteria associated with health and wellbeing. The conclusion is that prebiotics that currently fulfil these three criteria are fructo-oligosaccharides, galacto-oligosaccharides and lactulose, although promise does exist with several other dietary carbohydrates. Given the range of food vehicles that may be fortified by prebiotics, their ability to confer positive microflora changes and the health aspects that may accrue, it is important that robust technologies to assay functionality are used. This would include a molecular-based approach to determine flora changes. The future use of prebiotics may allow species-level changes in the microbiota, an extrapolation into genera other than the bifidobacteria and lactobacilli, and allow preferential use in disease-prone areas of the body.

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Section: Lactosucrosementioning

confidence: 99%

Dietary modulation of the human colonic microbiota: updating the concept of prebiotics

Gibson

Probert

Loo³

et al. 2004

Nutr. Res. Rev.

2,485

1,777

View full text Add to dashboard Cite

show abstract

“…The fructosyl moiety of sucrose forms a b -2,1 glycosidic bond to the glucose residue of lactose to create lactosucrose. This is done by transglycosylation via a b -fructofuranosidase enzyme, which produces a nonreducing oligosaccharide (Hara et al 1994 ) . This enzyme, however, also can hydrolyze sucrose (Mussatto and Mancilha 2007 ) .…”

Section: Manufacture Of Promising Prebioticsmentioning

confidence: 99%

Prebiotics of Plant and Microbial Origin

Boler

Fahey

2011

Direct-Fed Microbials and Prebiotics for Animals

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The food industry is constantly shifting focus based on what is most important to the consumer. Products are marketed currently that are believed to provide health benefi ts to the consumer such as benefi cial effects on health or as disease preventatives. Much of the focus is on oligosaccharides as health-promoting substrates. Many oligosaccharides are resistant to digestion and absorption by mammalian enzymes and, therefore, reach the large bowel where they may be fermented by the resident bacteria. Beyond their potential as substrates for fermentation, oligosaccharides are popular food additives due in large part to their low caloric value and their ability to enhance mineral absorption. Health benefi ts include alleviation of constipation, reduced risk of infection and diarrhea, and improved immune response. Many oligosaccharides modulate microbiota of the large bowel by increasing bifi dobacteria and lactobacilli populations and decreasing clostridia populations. This review will describe the manufacturing processes for select non-digestible oligosaccharides and other food ingredients currently classifi ed as prebiotics and those with prebiotic potential.

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“…Faecal amounts of short chain fatty acids (SCFAs) were analysed by high-performance liquid chromatography using the methods of Hara et al (11). Faecal concentrations of ammonia and sulfide were determined by potentiometer ILO-30 (DKK Co. Ltd, Tokyo, Japan) with an ammonia gas-sensing electrode (DKK Co. Ltd.) and with a sulfide electrode 7100 (DKK Co. Ltd) using the methods of Terada et al (12).…”

Section: Analysis Of Specimensmentioning

confidence: 99%

Effects of the consumption of heat-killedEnterococcus faecalisEC-12 preparation on microbiota and metabolic activity of the faeces in healthy adults

Terada

Bukawa²,

Kan³

et al. 2004

Microbial Ecology in Health and Disease

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The effects of the consumption (200 mg/day) of heat-killed Enterococcus faecalis EC-12 preparation (EC-12) for 14 days on microbiota and metabolic activity of the faeces were studied in eight healthy volunteers (22 Á/26 years of age). During EC-12 consumption, the counts of bifidobacteria (p B/0.05 on day 7 and p B/0.01 on day 14) were significantly increased, except for the numbers of bifidobacteria in one volunteer, whereas the counts and the frequency of occurrence of lecithinase-positive clostridia (p B/0.05), including Clostridium perfringens, were significantly decreased, when compared with the values before EC-12 consumption and during placebo consumption. The decreased tendency in the counts of Enterobacteriaceae and the increased tendency in the levels of lactobacilli was observed during EC-12 consumption, compared with the values before EC-12 consumption and during placebo consumption. No detectable changes occurred in the counts of other organisms throughout the experimental periods. The total faecal volatile fatty acid and acetic acid (p B/0.05 on day 7 and p B/0.01 on day 14) during EC-12 consumption, and succinic acid (p B/0.05), lactic acid (p B/0.05) and propionic acid (p B/0.05) on day 14 of EC-12 consumption were significantly increased when compared with the values before EC-12 consumption and during placebo consumption. Faecal concentrations of ammonia, phenol and p-cresol (p B/0.05) were significantly decreased on day 14 of EC-12 consumption. Faecal levels of sulfide and indole (p B/0.05) were significantly decreased during EC-12 consumption. Faecal values of oxidation-reduction potential (ORP; p B/0.05 on day 7 and p B/0.01 on day 14) were significantly decreased during EC-12 consumption. Faecal pH values tended to decrease on day 14 of EC-12 consumption. Stool weight and water content were slightly increased during EC-12 consumption. The odour of the faeces was slightly reduced during EC-12 consumption.

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Effective Dose of Lactosucrose on Fecal Flora and Fecal Metabolites of Humans

Cited by 64 publications

References 26 publications

Dietary modulation of the human colonic microbiota: updating the concept of prebiotics

Dietary modulation of the human colonic microbiota: updating the concept of prebiotics

Prebiotics of Plant and Microbial Origin

Effects of the consumption of heat-killedEnterococcus faecalisEC-12 preparation on microbiota and metabolic activity of the faeces in healthy adults

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