Effective elimination of Staphylococcal contamination from hospital surfaces by a bacteriophage–probiotic sanitation strategy: a monocentric study

D’Accolti, Maria; Soffritti, Irene; Lanzoni, Luca; Bisi, Matteo; Volta, Antonella; Mazzacane, Sante; Caselli, Elisabetta

doi:10.1111/1751-7915.13415

Cited by 24 publications

(45 citation statements)

References 30 publications

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“…After being thawed and vortexed, total DNA was extracted from 300 µL of each sample by the Exgene Cell SV Kit (Gene All, Tema Ricerca, Bologna, Italy), in a final elution volume of 100 µL. DNA samples were then analyzed by a customized qPCR microarray (BAID-00047RA Qiagen, Hilden, Germany), assessing simultaneously the presence of the following microbes: S. aureus, S. epidermidis, E. faecalis, E. faecium, E. coli, K. pneumonia/Enterobacter, A. baumannii, P. mirabilis, P. aeruginosa, C. perfringens, C. difficile, A, fumigatus and C. albicans, as previously reported [29]. An amount of 120 ng per sample (corresponding to 7 ng of template DNA per well/reaction) was used for the custom-array analysis.…”

Section: Molecular Analysesmentioning

confidence: 99%

Introduction of NGS in Environmental Surveillance for Healthcare-Associated Infection Control

et al. 2019

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The hospital environment significantly contributes to the onset of healthcare associated infections (HAIs), representing the most frequent and severe complications related to health care. The monitoring of hospital surfaces is generally addressed by microbial cultural isolation, with some performance limitations. Hence there is need to implement environmental surveillance systems using more effective methods. This study aimed to evaluate next-generation sequencing (NGS) technologies for hospital environment microbiome characterization, in comparison with conventional and molecular methods, in an Italian pediatric hospital. Environmental samples included critical surfaces of randomized rooms, surgical rooms, intensive care units and delivery rooms. The resistome of the contaminating population was also evaluated. NGS, compared to other methods, detected with higher sensitivity the environmental bacteria, and was the only method able to detect even unsearched bacteria. By contrast, however, it did not detect mycetes, nor it could distinguish viable from dead bacteria. Microbiological and PCR methods could identify and quantify mycetes, in addition to bacteria, and PCR could define the population resistome. These data suggest that NGS could be an effective method for hospital environment monitoring, especially if flanked by PCR for species identification and resistome characterization, providing a potential tool for the control of HAI transmission.

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Section: Molecular Analysesmentioning

confidence: 99%

Introduction of NGS in Environmental Surveillance for Healthcare-Associated Infection Control

et al. 2019

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“…Our results indicate that phages and S. epidermidis may be useful to treat atopic dermatitis and skin infection. Although the combinatorial use of probiotics and phages targeting S. aureus has been reported [ 30 , 31 ], to our knowledge, this is the first report of combined use for a potential treatment of S. aureus -associated skin infection. In this study, we used only a specific phage, SaGU1, in all experiments; nevertheless, we believe the results obtained here will be useful for other phage therapy research.…”

Section: Discussionmentioning

confidence: 99%

Staphylococcal Phage in Combination with Staphylococcus epidermidis as a Potential Treatment for Staphylococcus aureus-Associated Atopic Dermatitis and Suppressor of Phage-Resistant Mutants

Shimamori

Mitsunaka

Yamashita

et al. 2020

Viruses

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Atopic dermatitis is accompanied by the abnormal overgrowth of Staphylococcus aureus, a common cause of skin infections and an opportunistic pathogen. Although administration of antibiotics is effective against S. aureus, the resulting reduction in healthy microbiota and the emergence of drug-resistant bacteria are of concern. We propose that phage therapy can be an effective strategy to treat atopic dermatitis without perturbing the microbiota structure. In this study, we examined whether the S. aureus phage SaGU1 could be a tool to counteract the atopic exacerbation induced by S. aureus using an atopic mouse model. Administration of SaGU1 to the back skin of mice reduced both S. aureus counts and the disease exacerbation caused by S. aureus. Furthermore, the S. aureus-mediated exacerbation of atopic dermatitis with respect to IgE plasma concentration and histopathological findings was ameliorated by the application of SaGU1. We also found that Staphylococcus epidermidis, a typical epidermal symbiont in healthy skin, significantly attenuated the emergence of SaGU1-resistant S. aureus under co-culture with S. aureus and S. epidermidis in liquid culture infection experiments. Our results suggest that phage therapy using SaGU1 could be a promising clinical treatment for atopic dermatitis.

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“…In studies evaluating the actual potential of phages to be used as decontaminants during routine hospital cleaning, their usability was investigated when additioned to ecofriendly detergents (Probiotic Cleaning Hygiene System (PCHS)) [ 91 , 92 ]. In particular, the tested detergent also contained nonpathogenic probiotic bacteria belonging to the Bacillus genus, and it was used for many years for the routine cleaning of surfaces in several Italian hospitals.…”

Section: Bacteriophages As Environmental Sanitizers In Hospitalsmentioning

confidence: 99%

Bacteriophages as a Potential 360-Degree Pathogen Control Strategy

et al. 2021

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Bacteriophages are viruses that exclusively kill bacteria and are the most ubiquitous organisms on the planet. Since their discovery, bacteriophages have been considered an important weapon to fight human and animal infections of bacterial origin due to their specific ability to attack the associated target bacteria. With the discovery of antibiotics, phage treatment was progressively abandoned in Western countries. However, due to the recent emergence of growing antimicrobial resistance (AMR) to antibiotics, interest in phage use in human therapy has once again grown. Similarly, at the environmental level, the extensive use of disinfectants based on chemicals, including biocides in agriculture, has been associated with the emergence of resistance against disinfectants themselves, besides having a high environmental impact. Due to these issues, the applications of phages with biocontrol purposes have become an interesting option in several fields, including farms, food industry, agriculture, aquaculture and wastewater plants. Notably, phage action is maintained even when the target bacteria are multidrug resistant (MDR), rendering this option extremely interesting in counteracting AMR emergence both for therapeutical and decontamination purposes. Based on this, bacteriophages have been interestingly proposed as environmental routine sanitizers in hospitals, to counteract the spread of the pathogenic MDR bacteria that persistently contaminate hard surfaces. This review summarizes the studies aimed at evaluating the potential use of phages as decontaminants, with a special focus on hospital sanitation.

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Effective elimination of Staphylococcal contamination from hospital surfaces by a bacteriophage–probiotic sanitation strategy: a monocentric study

Cited by 24 publications

References 30 publications

Introduction of NGS in Environmental Surveillance for Healthcare-Associated Infection Control

Introduction of NGS in Environmental Surveillance for Healthcare-Associated Infection Control

Staphylococcal Phage in Combination with Staphylococcus epidermidis as a Potential Treatment for Staphylococcus aureus-Associated Atopic Dermatitis and Suppressor of Phage-Resistant Mutants

Bacteriophages as a Potential 360-Degree Pathogen Control Strategy

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