2017
DOI: 10.1017/s001667231600015x
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Effective experimental validation of miRNA targets using an improved linker reporter assay

Abstract: SummarymiRNAs are small, non-coding RNAs that play critical roles in various cellular processes. Although there are several algorithms that can predict the potential candidate genes that are regulated by a miRNA, these algorithms require further experimental validation in order to demonstrate genuine targets of miRNAs. Moreover, most algorithms predict hundreds to thousands of putative target genes for each miRNA, and it is difficult to validate all candidates using the whole 3′-untranslated region (UTR) repor… Show more

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Cited by 3 publications
(1 citation statement)
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“…A big drawback of computational prediction is its high false‐positive rate; multiple potential target sequences for each miRNA, and vice versa , can be falsely identified (Hausser & Zavolan, 2014). Thus, instead of depending on computational analysis, small RNA research is heavily reliant on experimental confirmation, especially using the classical luciferase reporter system (Choi et al ., 2017; Lemus‐Diaz et al ., 2017; Deng et al ., 2018).…”
Section: Introductionmentioning
confidence: 99%
“…A big drawback of computational prediction is its high false‐positive rate; multiple potential target sequences for each miRNA, and vice versa , can be falsely identified (Hausser & Zavolan, 2014). Thus, instead of depending on computational analysis, small RNA research is heavily reliant on experimental confirmation, especially using the classical luciferase reporter system (Choi et al ., 2017; Lemus‐Diaz et al ., 2017; Deng et al ., 2018).…”
Section: Introductionmentioning
confidence: 99%