Effective expression of the gene encoding an extracellular ribonuclease of Zinnia elegans in the SR1 Nicotiana tabacum plants

Sangaev, S. S.; Trifonova, E. A.; Титов, С. Е.; Romanova, A. V.; Kolodyazhnaya, Ya. S.; Komarova, M. L.; Sapotsky, M. V.; Malinovsky, V. I.; Кочетов, А. В.; Shumny, V. K.

doi:10.1134/s1022795407070186

Cited by 9 publications

(11 citation statements)

References 16 publications

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“…The apoplast contains several species of extracellular ribonucleases (ex-ribonucleases), which have been shown to target viral RNAs ( Sangaev et al, 2007 ; Sugawara et al, 2016 ; Potrokhov et al, 2021 ). Some of these ex-ribonucleases are induced in response to wounding and viral infection ( Kurata et al, 2002 ; LeBrasseur et al, 2002 ).…”

Section: Molecular Mechanisms Underlying Higsmentioning

confidence: 99%

Molecular mechanisms underlying host-induced gene silencing

Karimi

Innes

2022

The Plant Cell

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Host-induced gene silencing (HIGS) refers to the silencing of genes in pathogens and pests by expressing homologous double-stranded RNAs (dsRNA) or artificial microRNAs (amiRNAs) in the host plant. The discovery of such trans-kingdom RNA silencing has enabled the development of RNA interference-based approaches for controlling diverse crop pathogens and pests. Although HIGS is a promising strategy, the mechanisms by which these regulatory RNAs translocate from plants to pathogens, and how they induce gene silencing in pathogens, are poorly understood. This lack of understanding has led to large variability in the efficacy of various HIGS treatments. This variability is likely due to multiple factors, such as the ability of the target pathogen or pest to take up and/or process RNA from the host, the specific genes and target sequences selected in the pathogen or pest for silencing, and where, when, and how the dsRNAs or amiRNAs are produced and translocated. In this review, we summarize what is currently known about the molecular mechanisms underlying HIGS, identify key unanswered questions, and explore strategies for improving the efficacy and reproducibility of HIGS treatments in the control of crop diseases.

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Section: Molecular Mechanisms Underlying Higsmentioning

confidence: 99%

Molecular mechanisms underlying host-induced gene silencing

Karimi

Innes

2022

The Plant Cell

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“…The number of investigations devoted to transgenic plants with heterologous extracellular RNases is rather limited (Kochetov and Shumny, 2017). This approach provides an opportunity to obtain plants resistant to the broad spectrum of viruses (Sangaev et al, 2007;Trifonova et al, 2012). The benefits of the approach were proved by transgenic plants of a model species Nicotiana tabacum (Sangaev et al, 2011;Sugawara et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Increased ribonuclease activity in Solanum tuberosum L. transformed with heterologous genes of apoplastic ribonucleases as a putative approach for production of virus resistant plants

Potrokhov¹,

Sosnovska²,

Ovcharenko³

et al. 2021

Turk J Biol

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Viral pathogens seriously decrease the yield of potato ( Solanum tuberosum L.) –an important agricultural crop. Therefore, there is a demand for potato cultivars resistant to multiple viruses. Ribonucleases (RNases) are supposed to be engaged to antiviral response in plants. Heterologous RNase gene expression provides a tool for production of cultivars with multiple resistance to viruses and viroids. Transgenic potato cultivars Luhivs’ka and Lasynak with heterologous genes bov and ZRNase II of apoplastic RNases from Bos taurus and Zinnia elegans respectively were obtained via Agrobacterium -mediated transformation. The presence of bov and ZRNase II transgenes was confirmed by PCR analysis. RNase activity was examined by modified Oleshko method. Plants with heterologous ribonuclease genes had higher level of RNase activity compared to nontransgenic ones. Transgenic plants inoculated with Potato virus Y , PVY (genus Potyvirus , family Potyviridae) demonstrated delayed and less severe symptoms of viral infection. DAS-ELISA confirmed the presence of viral antigens both in transformed and control plants. Visual manifestations of viral infection in transgenic potatoes were milder than in control plants and their development was delayed, but complete elimination of the virus did not occur.

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“…Antisense suppression of the gene activity [1][2][3] or hyperexpres sion under the control of a strong promoter (fre quently of viral origin) [4][5][6] are the most frequently used methods for the study of gene functions. How ever, both of these approaches have at least one obvi ous shortcoming (the laboriousness of transgenic plant obtainment).…”

Section: Introductionmentioning

confidence: 99%

Virus-induced silencing as a method for studying gene functions in higher plants

et al. 2015

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The method of virus induced gene silencing (VIGS) based on posttranscriptional gene silencing (PTGS) is a promising new method for the study of plant gene functions. In the current review, we analyzed works on the development and improvement of this method, including the creation of new viral constructions for different plant species, the search for new reporter genes for the control of VIGS efficiency, and the devel opment of new, efficient methods of infection.

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Effective expression of the gene encoding an extracellular ribonuclease of Zinnia elegans in the SR1 Nicotiana tabacum plants

Cited by 9 publications

References 16 publications

Molecular mechanisms underlying host-induced gene silencing

Molecular mechanisms underlying host-induced gene silencing

Increased ribonuclease activity in Solanum tuberosum L. transformed with heterologous genes of apoplastic ribonucleases as a putative approach for production of virus resistant plants

Virus-induced silencing as a method for studying gene functions in higher plants

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