Effective immobilization of glucose oxidase on chitosan submicron particles from gladius of Todarodes pacificus for glucose sensing

Anusha, J.R.; Fleming, Albin T.; Kim, Heeje; Kim, Byung Chul; Yu, Kook Hyun

doi:10.1016/j.bioelechem.2015.02.004

Cited by 24 publications

(13 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…An efficient and sensitive glucose biosensor with good sensitivity can be fabricated by immobilizing GOx with an appropriate electrochemical transducer212429303132. Physical adsorption (drop casting method) is the simplest of all the immobilizing techniques to incorporate enzymes to transducers without affecting their native conformation, and this technique is commonly used for biosensors in the research stage33.…”

mentioning

confidence: 99%

Novel amperometric glucose biosensor based on MXene nanocomposite

Rakhi

Nayak

Xia

et al. 2016

Sci Rep

317

187

View full text Add to dashboard Cite

A biosensor platform based on Au/MXene nanocomposite for sensitive enzymatic glucose detection is reported. The biosensor leverages the unique electrocatalytic properties and synergistic effects between Au nanoparticles and MXene sheets. An amperometric glucose biosensor is fabricated by the immobilization of glucose oxidase (GOx) enzyme on Nafion solubilized Au/ MXene nanocomposite over glassy carbon electrode (GCE). The biomediated Au nanoparticles play a significant role in facilitating the electron exchange between the electroactive center of GOx and the electrode. The GOx/Au/MXene/Nafion/GCE biosensor electrode displayed a linear amperometric response in the glucose concentration range from 0.1 to 18 mM with a relatively high sensitivity of 4.2 μAmM−1 cm−2 and a detection limit of 5.9 μM (S/N = 3). Furthermore, the biosensor exhibited excellent stability, reproducibility and repeatability. Therefore, the Au/MXene nanocomposite reported in this work is a potential candidate as an electrochemical transducer in electrochemical biosensors.

show abstract

mentioning

confidence: 99%

Novel amperometric glucose biosensor based on MXene nanocomposite

Rakhi

Nayak

Xia

et al. 2016

Sci Rep

317

187

View full text Add to dashboard Cite

show abstract

“…Chitosan also demonstrates huge varieties of applications in following fields: In biomedical field for drug delivery, waste water treatment, as food additives, preservatives, as catalyst support, and for other analytical applications in a variety of industries such as pharmaceuticals, biochemistry, biotechnology, cosmetic, paper, and textile industries and other (Anusha et al 2015). It has distinctive applications of bioconversion used for the production of value-added food products, development of biodegradable films, improvement of waste matter as of food dealing out discards, decontamination of water, and de-acidification of fruit juices (Arancibia et al 2014;Thakker et al 2014).…”

Section: Chitosan: Electrochemical Sensing Materialsmentioning

confidence: 99%

Polymer-Based Biomaterials: An Emerging Electrochemical Sensor

Pandey

Jain

2020

Handbook of Polymer and Ceramic Nanotechnology

View full text Add to dashboard Cite

Driven by the increasing interest in nanotechnology, materials that spontaneously form ordered nanostructures are becoming an important area of investigation. The development of new polymer-based biomaterials relies on knowledge of the underlying relations between the properties and the structure of these materials at multiple length scales. On the basis of sources, biomaterial polymer films are categorized into two different classes. In the first class, polymers are made from protein, natural rubber, cellulose, etc. these are also called natural polymers. The second category is largest one; it carries the artificial polymer or synthesized polymer, where natural polymers are chemically treated or synthesized in lab. Among these, chitosan is widely used linear polysaccharide biopolymer derived from chitin by deacetylation, chitosan has numerous advantages as a biomaterial; Chitosan is a unique biopolymer in the respect that it is abundant, cationic, lowtoxic, non-immunogenic, biodegradable, and due to its positive charges at

show abstract

“…The biosensor fabricated under optimal conditions had a fast response time, superior sensitivity and long-term stability of over 60 days with good substrate selectivity which again owing to the CS s unique properties [85]. The submicron particles of chitosan from gladius of Todarodes pacificus were produced by ball milling technique, they showed high affinity and good biocompatibility for GOx [86]. In this case, CS was derived from β-chitin using deacetylation procedure which was followed by ball mill pulverization to form an ultra-fine white powder.…”

Section: Gox-chitosan Electrochemistrymentioning

confidence: 99%

“…An effective biocompatible environment to facilitate electronic communication with improved catalytic nature was possible due to the porous zinc oxide/platinum nanoparticle (ZnO/Pt)NPs-based electrode. Submicron particles from gladius of Todarodes pacificus (GCSPs) with porous structure effectively promoted the immobilization of enzyme by providing comparatively larger conductive surface and more active sites for the GOx molecules [86]. Besides, the CT-based paste was made by mixing graphite powder, GOx, Pt powder, and Nujol, and this paste filled the cavity of the carbon electrode [59].…”

Section: Electrode Material/refilling Matrixmentioning

confidence: 99%

Exploration of Chitinous Scaffold-Based Interfaces for Glucose Sensing Assemblies

Bagal-Kestwal

Chiang

2019

Polymers

View full text Add to dashboard Cite

The nanomaterial-integrated chitinous polymers have promoted the technological advancements in personal health care apparatus, particularly for enzyme-based devices like the glucometer. Chitin and chitosan, being natural biopolymers, have attracted great attention in the field of biocatalysts engineering. Their remarkable tunable properties have been explored for enhancing enzyme performance and biosensor advancements. Currently, incorporation of nanomaterials in chitin and chitosan-based biosensors are also widely exploited for enzyme stability and interference-free detection. Therefore, in this review, we focus on various innovative multi-faceted strategies used for the fabrication of biological assemblies using chitinous biomaterial interface. We aim to summarize the current development on chitin/chitosan and their nano-architecture scaffolds for interdisciplinary biosensor research, especially for analytes like glucose. This review article will be useful for understanding the overall multifunctional aspects and progress of chitin and chitosan-based polysaccharides in the food, biomedical, pharmaceutical, environmental, and other diverse applications.

show abstract

Effective immobilization of glucose oxidase on chitosan submicron particles from gladius of Todarodes pacificus for glucose sensing

Cited by 24 publications

References 46 publications

Novel amperometric glucose biosensor based on MXene nanocomposite

Novel amperometric glucose biosensor based on MXene nanocomposite

Polymer-Based Biomaterials: An Emerging Electrochemical Sensor

Exploration of Chitinous Scaffold-Based Interfaces for Glucose Sensing Assemblies

Contact Info

Product

Resources

About