1989
DOI: 10.1016/0014-5793(89)80663-5
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Effective method for obtaining long nucleotide chains on partially complementary templates Processed bovine γ‐interferon gene obtained from human γ‐interferon gene

Abstract: The method of obtaining the bovine 7-interferon gene by means of simultaneous multidirected mutagenesis of the human 7-interferon gene is presented. The first strand of the bovine y-interferon gene was obtained by ligation of synthetic oligonucleotides, using the cDNA of human 7-interferon, cloned in the single-stranded phage M13mpl9 as a template. The second strand was synthesized using a large fragment of E. coli DNA-polymerase I. The double-stranded gene was then treated by restriction nucleases and cloned … Show more

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Cited by 2 publications
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“…than half the amount of synthetic DNA, as does total gene synthesis, and does not require convenient restriction sites in the target DNA. Our method appears to be simpler and more reliable than a similar protocol recently reported (42). Transient expression of humAb4D5 in human embryonic kidney 293 cells permitted the isolation of 0.2-to 0.5-mg humAb4D5 variants for rapid characterization by growth inhibition and antigen binding affinity assays.…”
Section: Resultsmentioning
confidence: 90%
“…than half the amount of synthetic DNA, as does total gene synthesis, and does not require convenient restriction sites in the target DNA. Our method appears to be simpler and more reliable than a similar protocol recently reported (42). Transient expression of humAb4D5 in human embryonic kidney 293 cells permitted the isolation of 0.2-to 0.5-mg humAb4D5 variants for rapid characterization by growth inhibition and antigen binding affinity assays.…”
Section: Resultsmentioning
confidence: 90%