Effective Method to Deliver Test Substance in Adult Zebrafish (Danio Rerio).

Dayal, Navami; Joshi, Deeksha; Thakur, Mansee; Soparkar, Ameya; Doktor, Masooma; Patil, Poonam

doi:10.21474/ijar01/898

Cited by 8 publications

(4 citation statements)

References 8 publications

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“…Successful drug delivery was confirmed by measuring the drug blood levels [131]. In order to avoid the regurgitation of the administered solution, Dayal et al [141] slightly modified the oral administration technique implemented by Kulkarni et al [131] by using a butterfly needle which expedites the drug passage into the esophagus. A study by Cocchiaro et al introduced a needle-based micro gavage in zebrafish larvae using embryonic microinjection and stereomicroscopy equipment for the direct intestinal loading of substances [138,142,143].…”

Section: Oral Administrationmentioning

confidence: 99%

Assessing Drug Administration Techniques in Zebrafish Models of Neurological Disease

Chaoul,

Dib,

Bedran

et al. 2023

IJMS

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Neurological diseases, including neurodegenerative and neurodevelopmental disorders, affect nearly one in six of the world’s population. The burden of the resulting deaths and disability is set to rise during the next few decades as a consequence of an aging population. To address this, zebrafish have become increasingly prominent as a model for studying human neurological diseases and exploring potential therapies. Zebrafish offer numerous benefits, such as genetic homology and brain similarities, complementing traditional mammalian models and serving as a valuable tool for genetic screening and drug discovery. In this comprehensive review, we highlight various drug delivery techniques and systems employed for therapeutic interventions of neurological diseases in zebrafish, and evaluate their suitability. We also discuss the challenges encountered during this process and present potential advancements in innovative techniques.

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Section: Oral Administrationmentioning

confidence: 99%

Assessing Drug Administration Techniques in Zebrafish Models of Neurological Disease

Chaoul,

Dib,

Bedran

et al. 2023

IJMS

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“…However, the insoluble nature of graphene makes it difficult to measure the exact delivery dose. Other more precise delivery methods like oral delivery can be considered in the future [115]. Hence, to establish the toxicity criteria further, more studies are considered necessary to understand how graphene and GO can induce toxicity.…”

Section: Current Understanding Of Graphene and Graphene Oxide (Go) Tomentioning

confidence: 99%

Toxicity Studies on Graphene-Based Nanomaterials in Aquatic Organisms: Current Understanding

et al. 2020

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Graphene and its oxide are nanomaterials considered currently to be very promising because of their great potential applications in various industries. The exceptional physiochemical properties of graphene, particularly thermal conductivity, electron mobility, high surface area, and mechanical strength, promise development of novel or enhanced technologies in industries. The diverse applications of graphene and graphene oxide (GO) include energy storage, sensors, generators, light processing, electronics, and targeted drug delivery. However, the extensive use and exposure to graphene and GO might pose a great threat to living organisms and ultimately to human health. The toxicity data of graphene and GO is still insufficient to point out its side effects to different living organisms. Their accumulation in the aquatic environment might create complex problems in aquatic food chains and aquatic habitats leading to debilitating health effects in humans. The potential toxic effects of graphene and GO are not fully understood. However, they have been reported to cause agglomeration, long-term persistence, and toxic effects penetrating cell membrane and interacting with cellular components. In this review paper, we have primarily focused on the toxic effects of graphene and GO caused on aquatic invertebrates and fish (cell line and organisms). Here, we aim to point out the current understanding and knowledge gaps of graphene and GO toxicity.

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“…Therefore, in this study, the zebrafish ( Danio rerio ) has been selected as an animal test model due to high transparency, early life stage development, and cost reduction. 70% of genes which are associated with diseases in human body have functional homology in zebrafish [ 9 ]. While the early life stage of zebrafish (embryonic stage) can provide primary information, acute and chronic tests on adult zebrafish allow the observation of any sign regarding toxicity.…”

Section: Introductionmentioning

confidence: 99%

In Vivo Toxicity Evaluation of Sugar Adulterated Heterotrigona itama Honey Using Zebrafish Model

et al. 2021

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Honey is prone to be adulterated through mixing with sugars, cheap and low-quality honey, and other adulterants. Consumption of adulterated honey may cause several health issues such as weight gain, diabetes, and liver and kidney dysfunction. Therefore, studying the impact of consumption of adulterated honey on consumers is critical since there is a lack of study in this field. Hence, the aims of this paper were: (1) to determine the lethal concentration (LC50) of adulterated honey using zebrafish embryo, (2) to elucidate toxicology of selected adulterated honey based on lethal dose (LD50) using adult zebrafish, (3) to determine the effects of adulterated honey on histological changes of zebrafish, and (4) to screen the metabolites profile of adulterated honey by using zebrafish blood serum. The LC50 of Heterotrigona itama honey (acacia honey) and its sugar adulterants (light corn sugar, cane sugar, inverted sugar, and palm sugar in the proportion of 1–3% (w/w) from the total volume) was determined by the toxicological assessment of honey samples on zebrafish embryos (different exposure concentrations in 24, 48, 72, and 96 h postfertilization (hpf)). Pure H. itama honey represents the LC50 of 34.40 ± 1.84 (mg/mL) at 96 hpf, while the inverted sugar represents the lowest LC50 (5.03 ± 0.92 mg/mL) among sugar adulterants. The highest concentration (3%) of sugar adulterants were used to study the toxicology of adulterated honey using adult zebrafish in terms of acute, prolong-acute, and sub-acute tests. The results of the LD50 from the sub-acute toxicity test of pure H. itama honey was 2.33 ± 0.24 (mg/mL). The histological studies of internal organs showed a lesion in the liver, kidney, and spleen of adulterated treated-honey groups compared to the control group. Furthermore, the LC-MS/MS results revealed three endogenous metabolites in both the pure and adulterated honey treated groups, as follows: (1) S-Cysteinosuccinic acid, (2) 2,3-Diphosphoglyceric acid, and (3) Cysteinyl-Tyrosine. The results of this study demonstrated that adulterated honey caused mortality, which contributes to higher toxicity, and also suggested that the zebrafish toxicity test could be a standard method for assessing the potential toxicity of other hazardous food additives. The information gained from this research will permit an evaluation of the potential risk associated with the consumption of adulterated compared to pure honey.

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Effective Method to Deliver Test Substance in Adult Zebrafish (Danio Rerio).

Cited by 8 publications

References 8 publications

Assessing Drug Administration Techniques in Zebrafish Models of Neurological Disease

Assessing Drug Administration Techniques in Zebrafish Models of Neurological Disease

Toxicity Studies on Graphene-Based Nanomaterials in Aquatic Organisms: Current Understanding

In Vivo Toxicity Evaluation of Sugar Adulterated Heterotrigona itama Honey Using Zebrafish Model

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