After many attempts for Dye Sensitized Solar Cell (DSSC) efficiency improvement which mainly concentrated on electrode materials, sensitizers, and electrolytes, however there are only a few reports that have focused on the improvement of the interface between substrate material and semiconductor material. Therefore, the main objective of this work is to investigate the effect of surface treatment to the DSSC power conversion efficiency (η%) by employing different steps in pre-treatment process using sandpaper and hydrogen peroxide (H2O2) on Titanium (Ti) foil photoanode substrate. The substrates were labelled as Untreated (substrate was cleaned without sandpaper scratching and H2O2 pre-treatment), Scratched (undergone sandpaper scratching without H2O2 pre-treatment) and H2O2 Treated (Pre-treatment with both sandpaper scratching and H2O2) respectively. The Ti foil sheet resistance of each treatment process was measured using four-point probe. The morphological surface of substrates were determined using Field Emission Scanning Electron Microscopy (FESEM) whereas the elemental analysis was obtained from Energy Dispersive X-Ray (EDX). The morphological images show that the surface roughness increased after each pre-treatment process, resulting in the decrease in the sheet resistance value which contributes to a better electron transfer process, thus leading to improvement on DSSC efficiency (η%). This is proven from the I-V characteristics analysis where the H2O2 Treated Ti foil shows an impressive improvement with the highest efficiency (η%) of 1.12%.