Effective Radii of Macromolecules in Dilute Polyvinyl Alcohol Solutions

Khorolskyi, O.V.

doi:10.15407/ujpe63.2.144

Cited by 13 publications

(19 citation statements)

References 11 publications

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“…Up to and inclusive the crossover concentration * = 3.7 wt%, which determines the transition from dilute solutions to semidilute ones and which was calculated from the characteristic viscosity by formulas (5) and (6), the effective radii of albumin molecules remain invariant and equal to 44Å within the whole temperature interval. A similar situation was described by us earlier in work [15] for the effective radii of polyvinyl alcohol (PVA) macromolecules in dilute aqueous solutions. In particular, a "plateau" of effective macromolecular radii was observed in the region of relatively low temperatures and concentrations, where the effective radii remained constant.…”

Section: Discussion Of the Results Obtainedsupporting

confidence: 75%

“…These are characteristic curves that make it possible to detect changes in the size of an albumin macromolecule associated with the water properties in the solution bulk and near the macromolecule surface. It was shown earlier in work [15] that the characteristic curves for aqueous PVA solutions can be approximated by two straight lines with different slopes and intersecting each other at a temper-ature of (315 ± 2) K. A conclusion was drawn that this is a temperature, at which the microscopic properties of the liquid system PVA-water change, and these changes are induced by changes in the properties of the solvent, i.e. water.…”

Section: Discussion Of the Results Obtainedmentioning

confidence: 90%

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Calculation of the Effective Macromolecular Radii of Human Serum Albumin from the Shear Viscosity Data for Its Aqueous Solutions

Khorolskyi

2019

Ukr. J. Phys.

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The Malomuzh–Orlov theory is used to analyze the experimental shear viscosity data obtained for aqueous solutions of human serum albumin (HSA) at pH = 7.0 in wide temperature and concentration intervals, which allowed the effective radii of HSA macromolecules to be calculated. It is shown that three intervals of the effective molecular radius of HSA with different behaviors can be distinguished in a temperature interval of 278–318 K: 1) below the crossover concentration, the effective molecular radius of HSA remains constant; 2) in the interval from the crossover concentration to about 10 wt%, the effective molecular radius of HSA in the aqueous solution nonlinearly decreases; and 3) at concentrations of 10.2–23.8 wt%, the effective radius of HSA macromolecules linearly decreases, as the concentration grows. The assumption is made that the properties of water molecules in the solution bulk play a crucial role in the dynamics of HSA macromolecules at the vital concentrations of HSA in the solutions. The role of water near the surface of HSA macromolecules and the corresponding changes of its physical properties have been discussed.

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Section: Discussion Of the Results Obtainedsupporting

confidence: 75%

Section: Discussion Of the Results Obtainedmentioning

confidence: 90%

Calculation of the Effective Macromolecular Radii of Human Serum Albumin from the Shear Viscosity Data for Its Aqueous Solutions

Khorolskyi

2019

Ukr. J. Phys.

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“…Let us evaluate the volume fraction of proteins in blood plasma. According to the Khorolsky works [13][14][15], the effective radius of an isolated albumin macromolecule is approximately equal to where alb = × 1.66 × 10 −24 g is the mass of an albumin molecule. Substituting the indicated values for the density and size of albumin macromolecules, we obtain the following estimate: alb ≈ 0.2, which is close to the percolation threshold value (see below).…”

Section: Discussion Of Resultsmentioning

confidence: 99%

Characteristic Changes in the Density and Shear Viscosity of Human Blood Plasma with Varying Protein Concentration

et al. 2020

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The density and shear viscosity of human blood plasma and their dependence on the concentration of proteins (albumin, y-globulin, fibrinogen, etc.) entering the natural blood composition have been studied. The biomaterial concentration is varied by diluting the blood plasma with the isotonic aqueous solution. It is shown that a decrease in the biomaterial concentration down to 0.91 of its initial value leads to a drastic change in the plasma density and to a change in the character of the concentration dependence of the shear viscosity of blood plasma. A hypothesis is put forward that the observed changes in the density and shear viscosity result from the structural transformations induced by oligomerization processes; first of all, by the albumin dimerization. A conclusion is drawn that the introduced blood substitutes should not exceed 10% of the blood mass; otherwise, structural transformations of a biomaterial in blood plasma can be provoked.

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“…The latter considers the rotational degrees of particle freedom. The method of the Malomuzh-Orlov algorithm application was described in works [11,12] in detail. This algorithm allows the viscosity behavior of dilute macromolecular solutions to be described within the interval of bulk particle concentrations ≤ 0.5, i.e.…”

Section: Application Of the Malomuzh-orlov Formula To The Shear Viscomentioning

confidence: 99%

“…This algorithm allows the viscosity behavior of dilute macromolecular solutions to be described within the interval of bulk particle concentrations ≤ 0.5, i.e. up to concentrations that, in essence, coincide with the solution concentrations at which all macromolecules are in contact with one another [9][10][11][12].…”

Section: Application Of the Malomuzh-orlov Formula To The Shear Viscomentioning

confidence: 99%

Calculation of the Macromolecular Size of Bovine Serum Albumin from the Viscosity of Its Aqueous Solutions

Khorolskyi

Moskalenko

2020

Ukr. J. Phys.

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On the basis of experimental data for the shear viscosity of aqueous bovine serum albumin (BSA) solutions and in the framework of the Malomuzh–Orlov cellular approach, the surface of effective radii of BSA macromolecules has been plotted for the constant pH = 5.2 in the concentration interval of 2.0–27.2 wt% and the temperature interval 278–318 K. A rapid nonlinear increase in the effective radii of BSA macromolecules is shown to take place up to BSA concentrations of about 5 wt% in the whole examined temperature interval. The maxima of the effective radii of BSA macromolecules are observed at a BSA concentration of 5 wt%, and their position is temperature-independent. In the concentration interval 5.0–27.2 wt%, the effective radii of BSA macromolecules decrease, and this reduction is linear at BSA concentrations higher than 10 wt%. A comparison of the calculation results with literature data on the self-diffusion coefficient of macromolecules in solutions testifies to the efficiency of the Malomuzh–Orlov formula for calculating the macromolecular radii of globular proteins on the basis of shear viscosity data for their aqueous solutions.

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Effective Radii of Macromolecules in Dilute Polyvinyl Alcohol Solutions

Cited by 13 publications

References 11 publications

Calculation of the Effective Macromolecular Radii of Human Serum Albumin from the Shear Viscosity Data for Its Aqueous Solutions

Calculation of the Effective Macromolecular Radii of Human Serum Albumin from the Shear Viscosity Data for Its Aqueous Solutions

Characteristic Changes in the Density and Shear Viscosity of Human Blood Plasma with Varying Protein Concentration

Calculation of the Macromolecular Size of Bovine Serum Albumin from the Viscosity of Its Aqueous Solutions

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