This paper presents a study on the effect of the ratio of biodiesel and injection timing on the performance of diesel engines and their emissions. The research engine is a cylinder engine AVL-5402, simulated by software AVL-Boost. The simulated fuel includes fossil diesel and biodiesel blended with a replacement rate from 0% to 50%, with a simulation mode of 2200 (rev/min), at a rate of a 25%, 50% and 75% load. In this speed range, the engine has the lowest fuel consumption. The parameters to be evaluated are power, fuel consumption and emissions, based on the proportions of blended biodiesel. The results show that there is a relationship between the proportion of blended biodiesel, injection timing and the parameters of the engine. Specifically, the ratio of the biodiesel blend increases, injection timing tends to move closer to the top dead center (TDC), the tendency reduce engine power, fuel consumption increases, the emissions of CO and soot reduces, while NOx increases.