effective role of vitamin D and omega-3 on rats with induced Crohn’s disease

Abstract

Crohn's disease is an irreversible inflammatory disease, mainly affecting the gastrointestinal tract. The aim of present study was to investigate the effect of dietary vitamin D and omega 3 supplementation on rats with induced Crohn’s disease. Twenty – four adult male albino rats weighing (160 gm) were divided into 4 groups. The 1st group (n=6) was fed on the basal diet. The 2nd to 4th of rats (n=18) were injected with (10 mg/kg for 3 days) of indomethacin at the end of the experiment to induce Crohn’s disease. Groups (3 and 4) were fed on basal diet and given orally 1000/IU/kg/ b.w. of vitamin D and 600 mg/kg/b.w. of omega 3 respectively. At the end of the experimental period (8 weeks), rats were scarified. Biochemical analysis of the tested parameters was determined. The Results indicated that indomethacin Crohn’s rats showed a significant (P< 0.05) decrease in serum albumin, serum Catalase and serum Glutathione peroxidase compared to the negative control one, while serum liver function, MDA, CRP, and immunity proteins were significantly increased.