Agricultural activities are the second largest source of greenhouse gas emissions, and carbon emissions from agricultural land use (CEALU) have become a hot issue across the world. Although there are some studies on the impact of high-standard farmland construction policies on carbon emissions, they focus on quantitative analysis and do not give sufficient consideration to the relationship between HSFC and CEALU. Therefore, in this study, by relying on provincial panel data of China for the period 2005–2017, the effect of the high-standard basic farmland construction policy on carbon emissions from agricultural land use per unit area and its regional differences were quantitatively analyzed using the difference-in-difference (DID) model. The results showed that: (1) China’s CEALU per unit area presented a fluctuating upward change, but the growth rate slowed down during the period 2005–2017, from 392.58 kg/ha to 457.72 kg/ha, with an average annual growth rate of 1.31%; (2) the high-standard farmland construction (HSFC) policy led a significant carbon emission reduction effect in agricultural land use and reduced the CEALU per unit area by 10.80% on average. With the promotion of this policy, its carbon emission reduction effect in agricultural land use presented an overall increasing change; (3) the carbon emission reduction effect of the high-standard farmland construction policy in agricultural land use was significant in central China, but non-significant in eastern China and western China.