Effective Spore Density of &lt;i&gt;Glomus mosseae&lt;/i&gt;, Arbuscular Mycorrhiza (AM), for Inoculation of Rooted Cuttings of Black Pepper (&lt;i&gt;Piper nigrum&lt;/i&gt; Linn.)

Mala, WJ; Kumari, I. S.; Sumanasena, HA; Nanayakkara, C.M.

doi:10.4038/tar.v21i2.2600

Cited by 3 publications

(2 citation statements)

References 7 publications

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“…Endomikoriza bersifat simbion obligat yang tidak dapat melestarikan tubuh dan reproduksinya bila terpisah dari tanaman inang (Simanungkalit et al 2006;Parniske 2008;Hodge and Fitter 2010;Denison and Kiers 2011;Kheyrodin 2014). Umumnya produksi inokulan mikoriza dilakukan dengan cara kultur pot menggunakan tanaman inang (Chalimah et al 2007;Mala et al 2010;Marleen et al 2011;Herryawan 2012;Tamin et al 2012). Potongan akar yang mengandung mikoriza selain bisa disimpan di kultur pot, juga dapat disimpan di dalam bahan pembawa steril.…”

Section: Pendahuluanunclassified

Pengaruh Metode Sterilisasi Iradiasi Sinar Gamma Co-60 terhadap Bahan Pembawa dan Viabilitas Spora Gigaspora margarita

et al. 2020

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Proses sterilisasi digunakan untuk menghilangkan atau mengurangi mikrob yang tidak diinginkan pada bahan pembawa. Penelitian ini bertujuan untuk melihat pengaruh metode sterilisasi (autoklaf dan radiasi Gamma) terhadap viabilitas spora G. margarita dan kelarutan Fe 2+ , Mn 2+ , Zn 2+ pada bahan pembawa zeolit, kompos dan tanah. Penelitian menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari faktor jenis sterilisasi radiasi, autoklaf dan bahan pembawa kompos dan zeolit. Hasil penelitian menunjukkan bahwa sterilisasi autoklaf, radiasi sinar Gamma dosis 40 dan 50 kGy mampu mensterilkan bahan pembawa di zeolit sampai 0 cfu g-1 , sedangkan pada bahan pembawa kompos yang diradiasi dengan sinar Gamma dosis 50 kGy mampu mengurangi mikrob kontaminan sebanyak 76 kali dibandingkan menggunakan sterilisasi autoklaf. Penyimpanan satu bulan pasca sterilisasi menggunakan autoklaf pada zeolit viabilitas spora G. margarita tertinggi sebesar 46.95%, sedangkan untuk kompos tidak ada spora G. margarita yang berkecambah. Penyimpanan 3 bulan pasca sterilisasi pada radiasi dosis 10 kGy pada zeolit viabilitas spora G. margarita tertinggi sebesar 45,81%, sedangkan pada kompos tidak ada spora G. margarita yang berkecambah. Jenis sterilisasi menunjukkan pada bahan pembawa zeolit dan tanah tidak berpengaruh sedangkan pada kompos berpengaruh terhadap kelarutan Fe 2+. Sterilisasi dengan radiasi dosis 50 kGy meningkatkan kelarutan Mn 2+ pada bahan pembawa zeolit. Sterilisasi dengan autoklaf meningkatkan kelarutan Mn 2+ pada tanah. Jenis sterilisasi pada tanah tidak berpengaruh terhadap Zn 2+ sedangkan pada zeolit dan kompos berpengaruh terhadap kelarutan Zn 2+. Peningkatan unsur mikro dapat berpengaruh buruk bagi viabilitas G. margarita.

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Section: Pendahuluanunclassified

Pengaruh Metode Sterilisasi Iradiasi Sinar Gamma Co-60 terhadap Bahan Pembawa dan Viabilitas Spora Gigaspora margarita

et al. 2020

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“…Black pepper is also identified as a host crop of mycorrhizae. Re-inoculation of nursery plants with AM isolates collected from different black pepper growing fields has been studied for good quality planting material production of black pepper (Mala et al, 2010). However, mycorrhizal activity in fields with mature black pepper plants of Sri Lanka is not properly explored and documented up to date.…”

Section: Introductionmentioning

confidence: 99%

Spore abundance and morphological root modifications of arbuscular mycorrhizal fungi-infected black pepper (<em>Piper nigrum</em> L) plants in reddish brown latesolic soil of Matale in Sri Lanka

Weerawardena¹,

Bandara²

2018

SL J Food & Agric

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Arbuscular Mycorrhizae (AM) is a symbiotic association between fungi and roots of higher plants, which enhances plant growth and production. Black pepper (Piper nigrum L: Family Piperaceae), the second most valuable spice crop in Sri Lanka, is also identified as a host crop of mycorrhizae. However, existing indigenous AM species, their spore abundance and root colonization is not yet well studied or documented. Therefore, the present study was conducted to collect data on AM spore density and morphological features of modified roots due to infection of existing AM species in black pepper fields. Rhizosphere soils of randomly selected, 20 well-grown black pepper plants in RBL soils of Matale in Sri Lanka were evaluated for pH, moisture and major nutrients while AM spore abundance and AM colonization in stained roots were examined microscopically. The soil pH (4.7-5.9), total N (0.18-0.23 %), and organic C (1.57-1.75%) levels in study sites were preferable for biological activities while variability was observed in available P (4.4-29 ppm), exchangeable K (188-788 ppm) and volumetric moisture (9.8-24%). The AM spore (size >50 µm) density in examined soils were high and ranged from 1600-9000 spores kg-1 soil. Both live (slight yellowish to dark brown in colour) and dead (black) spores were present and globular or spherical in shape without any special hyphal attachments. About 95% of examined roots were infected with AM with modifications such as arbuscules or vesicles or both. Networks of non-septate, extra-radical hyphae and branched intra-radical hyphae could be seen. Arbuscules observed were in different maturity and highly branched. Both inter or intracellular vesicles found were oval or ellipsoid in shape. Thus, most of the mature black pepper plants in RBL soils of Matale were found infected with indigenous vesicular arbuscular mycorrhizae (VAM) and morphological features of their spores, hyphae, arbuscules and vesicles were similar to fungi of order Glomerales, which consisted with Glomus spp

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Perspectives on the Role of Arbuscular Mycorrhizal Fungi in the In Vivo Vegetative Plant Propagation

Ravi

Muthukumar

2019

Biofertilizers for Sustainable Agriculture and Environment

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Effective Spore Density of <i>Glomus mosseae</i>, Arbuscular Mycorrhiza (AM), for Inoculation of Rooted Cuttings of Black Pepper (<i>Piper nigrum</i> Linn.)

Abstract: ABSTRACT. Introduction of mycorrhizal associations is a complementary alternative to improve fertilizer absorption efficiency of black pepper (Piper nigrum Linn.

Cited by 3 publications

References 7 publications

Pengaruh Metode Sterilisasi Iradiasi Sinar Gamma Co-60 terhadap Bahan Pembawa dan Viabilitas Spora Gigaspora margarita

Pengaruh Metode Sterilisasi Iradiasi Sinar Gamma Co-60 terhadap Bahan Pembawa dan Viabilitas Spora Gigaspora margarita

Spore abundance and morphological root modifications of arbuscular mycorrhizal fungi-infected black pepper (<em>Piper nigrum</em> L) plants in reddish brown latesolic soil of Matale in Sri Lanka

Perspectives on the Role of Arbuscular Mycorrhizal Fungi in the In Vivo Vegetative Plant Propagation

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