Effective Targeting of DC-SIGN by α-Fucosylamide Functionalized Gold Nanoparticles

Arosio, Daniela; Chiodo, Fabrizio; Reina, José J.; Marelli, Marcello; Penadés, Soledad; Kooyk, Yvette van; García-Vallejo, Juan J.; Bernardi, Anna

doi:10.1021/bc500467u

Cited by 53 publications

(46 citation statements)

References 45 publications

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“…Targeting ligands [56][57][58][59][60] may be modified onto the AuNP surface to specifically deliver therapeutic cargos into tissues and organs. Recently, tumor-targeted mesoporous silicaencapsulated GNRs (GNRs@mSiO 2 ) for chemotherapy and photothermal therapy (PTT) have been developed by Shen et al 61 In this study, RGD peptides, a targeting ligand for α v β 3 integrin receptors that are known to overexpress on several cancer cells, were conjugated to the terminal groups of PEG on GNRs@mSiO 2 (namely, pGNRs@mSiO 2 -RGD).…”

Section: Surface Functionalization Of Aunpsmentioning

confidence: 99%

Gold nanoparticles enlighten the future of cancer theranostics

Guo¹,

Rahme²,

Yan³

et al. 2017

IJN

223

120

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Development of multifunctional nanomaterials, one of the most interesting and advanced research areas in the field of nanotechnology, is anticipated to revolutionize cancer diagnosis and treatment. Gold nanoparticles (AuNPs) are now being widely utilized in bioimaging and phototherapy due to their tunable and highly sensitive optical and electronic properties (the surface plasmon resonance). As a new concept, termed “theranostics,” multifunctional AuNPs may contain diagnostic and therapeutic functions that can be integrated into one system, thereby simultaneously facilitating diagnosis and therapy and monitoring therapeutic responses. In this review, the important properties of AuNPs relevant to diagnostic and phototherapeutic applications such as structure, shape, optics, and surface chemistry are described. Barriers for translational development of theranostic AuNPs and recent advances in the application of AuNPs for cancer diagnosis, photothermal, and photodynamic therapy are discussed

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Section: Surface Functionalization Of Aunpsmentioning

confidence: 99%

Gold nanoparticles enlighten the future of cancer theranostics

Guo¹,

Rahme²,

Yan³

et al. 2017

IJN

223

120

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“…These AuNPs induced pro-inflammation of MDDCs, as well as CD80, CD86, and HLA-DR upregulation. In addition, Arosio et al 39 synthesized AuNPs functionalized with α-fucosylamide, a molecule which facilitated DC-SIGN receptor targeting. These AuNPs were internalized by MDDCs via the DC-SIGN receptor without triggering immune responses, thus they can be utilized for delivery purposes.…”

Section: 23mentioning

confidence: 99%

Aerosol Delivery of Functionalized Gold Nanoparticles Target and Activate Dendritic Cells in a 3D Lung Cellular Model

et al. 2016

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Nanocarrier design combined with pulmonary drug delivery holds great promise for the treatment of respiratory tract disorders. In particular, targeting of dendritic cells that are key immune cells to enhance or suppress an immune response in the lung is a promising approach for the treatment of allergic diseases. Fluorescently encoded poly(vinyl alcohol) (PVA)-coated gold nanoparticles, functionalized with either negative (−COO − ) or positive (−NH 3 + ) surface charges, were functionalized with a DC-SIGN antibody on the particle surface, enabling binding to a dendritic cell surface receptor. A 3D coculture model consisting of epithelial and immune cells (macrophages and dendritic cells) mimicking the human lung epithelial tissue barrier was employed to assess the effects of aerosolized AuNPs. PVA-NH 2 AuNPs showed higher uptake compared to that of their −COOH counterparts, with the highest uptake recorded in macrophages, as shown by flow cytometry. None of the AuNPs induced cytotoxicity or necrosis or increased cytokine secretion, whereas only PVA-NH 2 AuNPs induced higher apoptosis levels. DC-SIGN AuNPs showed significantly increased uptake by monocyte-derived dendritic cells (MDDCs) with subsequent activation compared to non-antibody-conjugated control AuNPs, independent of surface charge. Our results show that DC-SIGN conjugation to the AuNPs enhanced MDDC targeting and activation in a complex 3D lung cell model. These findings highlight the potential of immunoengineering approaches to the targeting and activation of immune cells in the lung by nanocarriers.

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“…Ara h 1, Der p 1, Der p 2, Can f 1 and Cyn d 1 (Bermuda grass pollen) have been reported as ligands for DC-SIGN and/or DC-SIGNR [41,42]. DC-SIGN has been suggested to modulate human DC activation for Th1 cell polarization, or IL-10-mediated regulatory responses [40,43,44]. Interestingly, Furmonaviciene et al [45] showed that Der p 1 cleaves DC-SIGN and DC-SIGNR by its proteolytic activity and releases these receptors from the cell surface in human DCs, thereby promoting Th2 cell polarization.…”

Section: C-type Lectinsmentioning

confidence: 99%

What makes an allergen?

Scheurer

Toda

Vieths

2015

Clin Experimental Allergy

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SummaryAllergic diseases are an immune disorder reacting to certain type of allergen(s). Remarkably only a small number of proteins of the plant and animal proteome act as allergens. Therefore, allergens have been clustered according to their common structural, biochemical and functional features. Evidence has accumulated that some allergens possess intrinsic adjuvant properties to stimulate the innate immunity. The adjuvant properties appear to contribute to the allergenicity of the respective proteins, namely the ability to cause allergic sensitization in susceptible subjects or allergic reactions in sensitized individuals. Here, we discuss how allergens interact with the innate immune cells, in particular dendritic cells and epithelial cells, via binding to pattern recognition receptors, exhibiting proteolytic activities and/or inducting type 2 innate lymphoid cells (ILC2), thereby contributing to the sensitization and development of allergic diseases.

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Effective Targeting of DC-SIGN by α-Fucosylamide Functionalized Gold Nanoparticles

Cited by 53 publications

References 45 publications

Gold nanoparticles enlighten the future of cancer theranostics

Gold nanoparticles enlighten the future of cancer theranostics

Aerosol Delivery of Functionalized Gold Nanoparticles Target and Activate Dendritic Cells in a 3D Lung Cellular Model

What makes an allergen?

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