Effective use of Gamma Irradiation for Pathogen Inactivation of Monoclonal Antibody Preparations

Grieb, Teri; Forng, Ren-Yo; Brown, Robert S.; Owolabi, Timothy; Maddox, Ewa; D.G., McBAIN, A.J. Allison,; Drohan, William N.; Mann, David; Burgess, Wilson H.

doi:10.1006/biol.2002.0330

Cited by 70 publications

(64 citation statements)

References 7 publications

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“…A high temperature denatures viral functionally active proteins Virus inactivation at 'low' temperature (below 41°C) is considered to be caused by degradation of the nucleic acid [82,83] [ [84][85][86] Gamma irradiation Radiation Viruses are inactivated primarily by direct damage, via disruption of the genome Formation of free radicals that damage proteins [95] [95]…”

Section: Rna Degradationmentioning

confidence: 99%

“…The second pathway involves indirect damage via free radicals formed after breakage of covalent bonds. Viruses appear to be inactivated primarily by direct damage, via disruption of the genome [95].…”

Section: Gamma Irradiationmentioning

confidence: 99%

“…Gamma irradiation could inactivate PRRSV with the preservation of the viral entry-associated domains [41], but for other viruses the formation of free radicals should be investigated, as they might destroy the viral proteins [95].…”

Section: Gamma Irradiationmentioning

confidence: 99%

See 2 more Smart Citations

Inactivated virus vaccines from chemistry to prophylaxis: merits, risks and challenges

Delrue

Verzele

Madder

et al. 2012

Expert Review of Vaccines

166

150

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The aim of this review is to make researchers aware of the benefits of an efficient quality control system for prediction of a developed vaccine's efficacy. Two major goals should be addressed when inactivating a virus for vaccine purposes: first, the infectious virus should be inactivated completely in order to be safe, and second, the viral epitopes important for the induction of protective immunity should be conserved after inactivation in order to have an antigen of high quality. Therefore, some problems associated with the virus inactivation process, such as virus aggregate formation, protein crosslinking, protein denaturation and degradation should be addressed before testing an inactivated vaccine in vivo

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Section: Rna Degradationmentioning

confidence: 99%

Section: Gamma Irradiationmentioning

confidence: 99%

See 1 more Smart Citation

Inactivated virus vaccines from chemistry to prophylaxis: merits, risks and challenges

Delrue

Verzele

Madder

et al. 2012

Expert Review of Vaccines

166

150

View full text Add to dashboard Cite

show abstract

“…Conversely, other studies on bones and tendons [9,14,21,22] have claimed no significant reduction in biomechanical properties after similar doses. Nevertheless, a general consensus is that bones and tissues can be partially protected from free radical damage by treatment radioprotectants, i.e., deep freezing during radiation [19], or freeze drying and/or use of antioxicant ascorbate [23]. However, even though the impact of lower doses (e.g., 15 to 35 kGy) remains debatable, most studies agree that higher doses of gamma radiation (e.g., 40-60 kGy or more), which are expected to eliminate viral infection, can definitely lead to deleterious effects [8,10].…”

Section: Introductionmentioning

confidence: 99%

On the effect of X-ray irradiation on the deformation and fracture behavior of human cortical bone

Barth

Launey

MacDowell

et al. 2010

Bone

183

109

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In situ mechanical testing coupled with imaging using high-energy synchrotron x-ray diffraction or tomography imaging is gaining in popularity as a technique to investigate micrometer and even sub-micrometer deformation and fracture mechanisms in mineralized tissues, such as bone and teeth. However, the role of the irradiation in affecting the nature and properties of the tissue is not always taken into account. Accordingly, we examine here the effect of x-ray synchrotron-source irradiation on the mechanistic aspects of deformation and fracture in human cortical bone. Specifically, the strength, ductility and fracture resistance (both work-of-fracture and resistancecurve fracture toughness) of human femoral bone in the transverse (breaking) orientation were evaluated following exposures to 0.05, 70, 210 and 630 kGy irradiation. Our results show that the radiation typically used in tomography imaging can have a major and deleterious impact on the strength, post-yield behavior and fracture toughness of cortical bone, with the severity of the effect progressively increasing with higher doses of radiation. Plasticity was essentially suppressed after as little as 70 kGy of radiation; the fracture toughness was decreased by a factor of five after 210 kGy of radiation. Mechanistically, the irradiation was found to alter the salient toughening mechanisms, manifest by the progressive elimination of the bone's capacity for plastic deformation which restricts the intrinsic toughening from the formation "plastic zones" around crack-like defects. Deep-ultraviolet Raman spectroscopy indicated that this behavior could be related to degradation in the collagen integrity.

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“…Adopting this dual approach of irradiating proteins and enzymes at a low temperature in the presence of a free radical scavenger, insulin monoclonal antibody preparations were irradiated at 4°C at doses of 15 and 45 kGy in the presence and absence of 0.2 M ascorbate (Grieb et al 2002). In the absence of ascorbate, SDS PAGE and ELISA experiments showed that there was almost complete loss of activity and protein at both 15 and 45 kGy doses.…”

Section: Sterilisation Of Sensitive Biomolecules In Aqueous Solutionmentioning

confidence: 99%

Free radical studies of components of the extracellular matrix: contributions to protection of biomolecules and biomaterials from sterilising doses of ionising radiation

Parsons

2017

Cell Tissue Bank

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The purpose of the current review is show how the principles and techniques of radiation chemistry have enabled the direct reactions of free radicals with biomolecules and biomaterials to be investigated at the molecular level. In particular, the review focusses on the free radical-induced fragmentation of glycosaminoglycans. Glycosaminoglycans are large linear polysaccharides consisting of repeating disaccharide units and are important components of the extracellular matrix (ECM) either in free form (hyaluronan) or as a component of proteoglycans. Oxidative damage of the extracellular matrix components by either enzymatic or non-enzymatic pathways may have implications for the initiation and progression of a range of human diseases. These include arthritis, kidney disease, cardiovascular disease, lung disease, periodontal disease and chronic inflammation. Oxidative damage to hyaluronan by reactive oxidative species and thus the potential mechanism of damage to the ECM and its role in human pathologies is reviewed with particular focus on damage initiated by potential in vivo free radicals such as superoxide, carbonate and hydroxyl radicals. Such knowledge has also allowed radiation protecting systems to be developed so that sterilising doses of radiation can be delivered to sensitive biomolecules such as proteins and glycosaminoglycans, and also to sensitive biomaterials such as tissue allografts.

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Effective use of Gamma Irradiation for Pathogen Inactivation of Monoclonal Antibody Preparations

Cited by 70 publications

References 7 publications

Inactivated virus vaccines from chemistry to prophylaxis: merits, risks and challenges

Inactivated virus vaccines from chemistry to prophylaxis: merits, risks and challenges

On the effect of X-ray irradiation on the deformation and fracture behavior of human cortical bone

Free radical studies of components of the extracellular matrix: contributions to protection of biomolecules and biomaterials from sterilising doses of ionising radiation

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