Effective vaccination of mice against leprosy bacilli with subunits of Mycobacterium leprae

Gelber, Robert H.; Brennan, P. J.; Hunter, S W; Munn, M W; Monson, Janet M.; Murray, L P; Siu, P; Tsang, M; Engleman, Edgar G.; Mohagheghpour, Nahid

doi:10.1128/iai.58.3.711-718.1990

Cited by 33 publications

(13 citation statements)

References 22 publications

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“…Patients wilh active tuberculosis showed intermediate responses to cell wall proteins proportional to their responses to the whole leprosy bacillus. We also confirm that the cell wall proteins dieting these responses were able to stimulate protective immunity in mice against M. leprae foot-pad infection [13].…”

Section: Introductionsupporting

confidence: 75%

“…Protection against M. leprae infection can be induced by prior vaccination with live or dead M. leprae, BCG [24] or M. habana [25]. Recently, immunization with cell wall preparations of M. leprae, including extractable cell wail proteins, resulted in significant inhibition of the growth of M. leprae and corresponding T cell reactivity [13]. Although the degree of protection induced by immunization with the CWP in the present study was variable (Fig.…”

Section: Discussionmentioning

confidence: 67%

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Cellular immune response to the cell walls ofMycobacterium lepraein leprosy patients and healthy subjects exposed to leprosy

Roche

Neupane

Britton

1992

Clinical and Experimental Immunology

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SUMMARYCell walls of M. leprae coni\^{ of complex arrangements of carbohydrate, lipid. peptidoglycan and protein molecules. Recently, cxtractable proteins of a wide range of molecular weights were identified as components ofthe cell wall. We have examined the cellular immune responses of Nepali leprosy patients to a cell wall preparation of M. leprae enriched for these proteins. Strong lymphocyte proliferative responses to the antigens were present in halfof the paucibacillary leprosy patients and in the majority of healthy control subject

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Section: Introductionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 67%

Cellular immune response to the cell walls ofMycobacterium lepraein leprosy patients and healthy subjects exposed to leprosy

Roche

Neupane

Britton

1992

Clinical and Experimental Immunology

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“…In separate studies this protein was also found responsible for generation of high titres of specific antibodies in leprosy patients, having implications for diagnosis and patient care [33,42], However, an attempt to vaccinate mice with 35 kD protein against footpad challenge with Myco. leprae did not succeed [45], despite earlier encouraging results [46]. The 22 kD Myco.…”

Section: Discussionmentioning

confidence: 93%

Serological distinction of integral plasma membrane proteins as a class of mycobacterial antigens and their relevance for human T cell activation

Mehrotra

Bisht

Tiwari³

et al. 1995

Clinical and Experimental Immunology

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SUMMARYThis study pertains to classification and antigenic analysis of mycobacterial plasma membrane proteins in relation to human T cell proliferativc responses, using a 'fast grower' Mycobacterium fortuitum as model. Membrane vesicles, prepared hy sonicalion and differential centrifugation, were subjected to biphasic Triton X-114 extraction for isolation of integral (detergent phase) and peripheral (aqueous phase) proteins. Neither protein pool showed any appreciable overlap serologically. SDS-PAGE showed five prominent bands in peripheral and three in ihe integral protein pool, whereas immunohlotling with rahbit antisera identified only two major antigens {60 and 67 kD) in the former and five (24. 34, 42, 51 and 54 kD) in the latter, ELISA with a panel of anli-mycobacterial MoAbs revealed that nine out of 12 previously known antigens were present in the peripheral protein pool. Only two of them (33 and 40 kD) were additionally detected amongst integral proteins. The membrane-associated immunosuppressive moiety lipoarabinomannan was semiquantitatively located in aqueous phase. In bulk T cell proliferation assays, seven out of 10 subjects belonging to a 'respondcr" background (BT-BB leprosy palients and healthy contacts) showed high responses for Myco. fortttitum antigens. Proliterative response with integral proteins was comparable to thai with whole membrane, hut it was significanlly higher (/* < 0 0005) than the response with peripheral proteins. The distinction and relevance of integral membrane proteins as a class of mycobacterial antigens make them worthy of consideration in a subunit vaccine design.

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“…Though T lymphocytes play a major role in protection against facultative intracellular bacteria, antigens relevant to the humoral immune response have been studied more extensively than T-cell antigens because antigens recognized by antibodies are more technically amenable to study (40). To identify antigens recognized by T lymphocytes, complex protein preparations from intracellular bacteria have been used to investigate the cellular immune response (12,18,37). However, isolation of individual stimulatory components in complex mixtures is still difficult.…”

mentioning

confidence: 99%

Isolation of Brucella abortus ssb and uvrA genes from a genomic library by use of lymphocytes as probes

1993

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BruceUa abortus proteins from virulent S2308 expressed from a pBluescript II SK-genomic library stimulated peripheral blood mononuclear (PBM) cell proliferation from cattle vaccinated with B. abortus S19. The method described here permits a rapid and directed approach to isolate genes encoding antigens of B. abortus that interact with lymphocytes primed to the living bacterium. The supernatants from the bacterial host JM109 (DE3) were cultured with freshly isolated bovine PBM cells. A total of 300 clones were evaluated. Ten clones were identified that stimulated T-lymphocyte proliferation. Among them, one clone with a 2.5-kb insert stimulated T-lymphocyte proliferation in all three animals, suggesting that the proteins encoded by genes within this fragment may represent immunodominant antigens. DNA sequencing of this clone reveals two large open reading frames (ORFs). ORF II has a high degree of similarity to the Escherichia coli ssb gene, which codes for the single-stranded DNA binding protein. ORF I, in the opposite direction to ORF II, shows similarity to the N terminus of the E. coli uvrA gene, which codes for one of the three subunits of the E. coli ABC excision nuclease. The observation that the PBM cells recognized and proliferated in response to proteins expressed from single clones provides a novel strategy to select bacterial antigens that may prove useful in designing alternative vaccines against brucellosis.B. abortus genomic library. The library of B. abortus S2308, a virulent strain of B. abortus, was constructed in the HindIII restriction site of the pBluescript II SK-phagemid. 5339 on July 16, 2020 by guest http://iai.asm.org/ Downloaded from

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Effective vaccination of mice against leprosy bacilli with subunits of Mycobacterium leprae

Cited by 33 publications

References 22 publications

Cellular immune response to the cell walls ofMycobacterium lepraein leprosy patients and healthy subjects exposed to leprosy

Cellular immune response to the cell walls ofMycobacterium lepraein leprosy patients and healthy subjects exposed to leprosy

Serological distinction of integral plasma membrane proteins as a class of mycobacterial antigens and their relevance for human T cell activation

Isolation of Brucella abortus ssb and uvrA genes from a genomic library by use of lymphocytes as probes

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