Effectiveness and molecular characterization of Burkholderia spinosa, a prospective biocontrol agent for controlling postharvest diseases of banana

Costa, D. M. De; Zahra, A.R.F.; Kalpage, Maheshika; Rajapakse, E.M.G.

doi:10.1016/j.biocontrol.2008.08.010

Cited by 17 publications

(8 citation statements)

References 39 publications

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“…Combining morphological, physiological and biochemical characteristics with molecular information, the bacterial strain SD7 was identified as B. cepacia , while the actinomycetic strain NB20 was identified as S. katrae . Similarly, several species of Burkholderia have been reported as effective biocontrol agents for postharvest and soil‐borne plant diseases (de Costa, Zahra, Kalpage, & Rajapakse, ; Nguyen, Nicolaisen, Sorensen, & Olsson, ; Ren, Ye, Liu, Xu, & Wu, ; de los Santos‐Villalobos, Coyolxauhqui Barrera‐Galicia, Alberto Miranda‐Salcedo, & Jose Pena‐Cabriales, ; Sopheareth et al., ).…”

Section: Discussionmentioning

confidence: 99%

Identification and antifungal activity analysis of two biocontrol antagonists to Colletotrichum musae

Shu

Chen

et al. 2017

Journal of Phytopathology

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Postharvest anthracnose of banana caused by Colletotrichum musae is one of the major diseases resulting in huge economic losses worldwide. To control this disease using biocontrol agents, two antagonistic strains SD7 and NB20 with significant inhibitory effects on mycelial growth and conidial germination of C. musae were identified and evaluated in this study. The inhibitory effects of cell‐free culture filtrates of SD7 and NB20 on conidial germination of C. musae were both 100%, and those on mycelial growth of C. musae were 97.7 ± 0.9% and 95.0 ± 0.6%, respectively. The antifungal activities of cell‐free culture filtrates of both strains were still stable after they were stored at 4°C for 6 months. The control efficacies of cell‐free culture filtrates of SD7 and NB20 on postharvest anthracnose of banana were 55.9 ± 4.1% and 33.2 ± 3.9%, respectively. The disease severity (mean scale value) in banana fruit fingers was significantly lower after the treatment with a cultural suspension of the bacterial strain SD7 (1.4 ± 0.49) or actinomycete strain NB20 (2.0 ± 0.63), compared to that in the control (4.8 ± 0.40). After subculturing for 10 generations, the antifungal efficiency of NB20 remained stable, whereas that of strain SD7 declined obviously. Lastly, based on the morphological, physio‐biochemical and molecular characteristics, the bacterial strain SD7 was identified as Burkholderia cepacia, while the actinomycete strain NB20 was identified as Streptomyces katrae. The results from this study will provide the basis for developing an effective and novel biofungicide to control banana anthracnose disease.

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Section: Discussionmentioning

confidence: 99%

Identification and antifungal activity analysis of two biocontrol antagonists to Colletotrichum musae

Shu

Chen

et al. 2017

Journal of Phytopathology

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“…Several research groups have successfully applied Bcc-specific recA-based PCR test as a diagnostic tool for the identification of B. cepacia complex isolates in clinical (42,55) and environmental (56)(57)(58) settings. Specific recA primers have also been used to determine relatedness of potential biocontrol and environmental isolates to Bcc species (59)(60)(61). In fact, the PCR-based evaluation and sequencing of the recA gene have been identified as a powerful tool for identification and classification of Burkholderia isolates.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Burkholderia rinojensis sp. nov., a Non-Burkholderia cepacia Complex Soil Bacterium with Insecticidal and Miticidal Activities

Córdova-Kreylos

Fernandez

Koivunen

et al. 2013

Appl Environ Microbiol

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Isolate A396, a bacterium isolated from a Japanese soil sample demonstrated strong insecticidal and miticidal activities in laboratory bioassays. The isolate was characterized through biochemical methods, fatty acid methyl ester (FAME) analysis, sequencing of 16S rRNA, multilocus sequence typing and analysis, and DNA-DNA hybridization. FAME analysis matched A396 to Burkholderia cenocepacia, but this result was not confirmed by 16S rRNA or DNA-DNA hybridization. 16S rRNA sequencing indicated closest matches with B. glumae and B. plantarii. DNA-DNA hybridization experiments with B. plantarii, B. glumae, B. multivorans, and B. cenocepacia confirmed the low genetic similarity (11.5 to 37.4%) with known members of the genus. PCR-based screening showed that A396 lacks markers associated with members of the B. cepacia complex. Bioassay results indicated two mechanisms of action: through ingestion and contact. The isolate effectively controlled beet armyworms (Spodoptera exigua; BAW) and two-spotted spider mites (Tetranychus urticae; TSSM). In diet overlay bioassays with BAW, 1% to 4% (vol/vol) dilution of the whole-cell broth caused 97% to 100% mortality 4 days postexposure, and leaf disc treatment bioassays attained 75% ؎ 22% mortality 3 days postexposure. Contact bioassays led to 50% larval mortality, as well as discoloration, stunting, and failure to molt. TSSM mortality reached 93% in treated leaf discs. Activity was maintained in cell-free supernatants and after heat treatment (60°C for 2 h), indicating that a secondary metabolite or excreted thermostable enzyme might be responsible for the activity. Based on these results, we describe the novel species Burkholderia rinojensis, a good candidate for the development of a biocontrol product against insect and mite pests.

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“…B. spinosa was retrieved from stock cultures in sterile distilled water and maintained at the Laboratory of Plant Pathology and Microbiology, Department of Agricultural Biology, Faculty of Agriculture, and confirmed the purity and antagonism. Antagonism was confirmed by dual culture plate method (De Costa et al, 2008). B. spinosa cell suspensions were prepared in nutrient broth to have a final cell concentration of 10 8 CFU/ml.…”

Section: Microbial Culturesmentioning

confidence: 99%

“…When quantification of the microbial colonies, number of deep yellow colonies appearing on NA medium was quantified as B. spinosa colonies as they produce characteristic circular shiny deep yellow coloured colonies on NA. The identity of these colonies was confirmed by Gram staining, 3% KOH test and antagonism against C. musae by dual culture plate method (De Costa et al, 2008). Total bacterial, fungal and yeast colonies appearing on PDA plates were separately recorded based on colony and spore morphology.…”

Section: Data Collection and Analysismentioning

confidence: 99%

“…Burkholderia spinosa, a bacterium isolated from the peel tissues of banana has been proved for its antagonism against C. musae, under in vitro conditions and ability to reduce the rate of anthracnose development on different banana varieties when applied as a postharvest dip treatment (De Costa & Erabadupitiya, 2005: De Costa et al, 2008 The present study was conducted to determine the population dynamics of epiphytic and pathogenic microbes inhabiting on the phyllosphere when the banana plants were treated with B. spinosa as a preharvest foliar spray and a soil drench. Moreover, it assessed the retention/colonising efficiency of B. spinosa on leaf tissues of banana when the plants were treated by the two methods.…”

Section: Introductionmentioning

confidence: 99%

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Potential of pre-harvest application of Burkholderia spinosa for Biological control of epiphytic and pathogenic microorganisms on the phyllosphere of banana (Musa spp.)

Silva

Costa

2015

Trop. Agric. Res.

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A study was conducted to determine the effects of pre-harvest application of a bacterial antagonist, Burkholderia spinosa on the population dynamics of epiphytic and pathogenic microorganisms dwelling on banana (Musa spp.) leaves together with the population build-up of the applied bacterial antagonist. Banana cultivar Kolikuttu was established as an outdoor plot experiment and the antagonist was applied by two methods (i.e. a foliar spray and a soil drench) at nine times (i.e. 300 ml per plant per time) repeatedly at weekly intervals. Effects of leaf washings of plants treated with different application methods on germination of C. musae, the causal organism of several postharvest diseases of banana, spores were investigated in vitro. There was a significant (p<0.0001) interaction effect between time and method of B. spinosa application on the density of the bacterial antagonist on banana leaves. A gradual build-up of B. spinosa was observed on leaves with a peak density at 228 days after planting when bacterial antagonist was applied as a foliar spray. Burkholderia spinosa was not found on leaves of banana when the plants were treated as a soil drench or left untreated with the antagonist. Densities of Aspergilus spp. and unidentified fungal species on leaves of banana were significantly influenced by the method of application of B. spinosa. In contrast, density of Fusarium spp. on the banana phyllosphere varied significantly with the time of application (p<0.001). The density of yeast and unidentified bacterial species on banana leaves was not significantly influenced by the time and method of application or their interaction effect. Application of B. spinosa as a soil drench could reduce phyllosphere pathogens, Aspergillus spp. and Fusarium spp. Percentage germination of C. musae spores continued to increase 24 h after incubation in the leaf washing of control banana plants. In contrast, leaf washings of B. spinosa treated plants showed a decline in the percentage spore germination. The findings of the present study revealed the ability of B. spinosa for building up considerable cell densities on the treated leaf surface and its ability for suppressing a range of microbes. These are desirable features of B. spinosa to be used in field application for the control of foliar pathogens of banana.

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Effectiveness and molecular characterization of Burkholderia spinosa, a prospective biocontrol agent for controlling postharvest diseases of banana

Cited by 17 publications

References 39 publications

Identification and antifungal activity analysis of two biocontrol antagonists to Colletotrichum musae

Identification and antifungal activity analysis of two biocontrol antagonists to Colletotrichum musae

Isolation and Characterization of Burkholderia rinojensis sp. nov., a Non-Burkholderia cepacia Complex Soil Bacterium with Insecticidal and Miticidal Activities

Potential of pre-harvest application of Burkholderia spinosa for Biological control of epiphytic and pathogenic microorganisms on the phyllosphere of banana (Musa spp.)

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