Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones

Abstract

The aim of this study was to evaluate the effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones contaminated in vitro with Escherichia coli, Staphylococcus aureus, Streptococcus mutans, Candida albicans and Bacillus subtilus (in spore form). Two hundred and twenty-five gutta-percha cones were contaminated with standardized suspensions of each microorganism and incubated at 37°C for 24 h. The cones were divided into 10 experimental groups (n = 15), according to the microorganism tested and disinfection testing times. The disinfection procedure consisted of immersing each cone in a plastic tube containing the substance. The specimens remained in contact with the substance for 1 or 2.5 minutes. Afterwards, each cone was transferred to a 10% sodium thiosulphate solution (Na 2 S 2 O 3 ) to neutralize the disinfectant. Microbial biofilms adhering to the cones were dispersed by agitation. Aliquots of 0.1 ml of the suspensions obtained were plated on Sabouraud dextrose agar, or brain and heart infusion agar, and incubated at 37°C for 24 h. The results were expressed in colony forming units (CFU/ml) and the data were submitted to the Wilcoxon Signed Rank Test (level of significance at 0.05). A significant reduction was observed, after 1 minute of exposure, in the test solution for C. albicans (p = 0.0190), S. aureus (p = 0.0001), S. mutans (p = 0.0001), B. subtilis (p = 0.0001), and E. coli (p = 0.0001). After 2.5 minutes of exposure, 100% of the microbial inocula were eliminated. It was concluded that the 2% peracetic acid solution was effective against the biofilms of the tested microorganisms on gutta-percha cones at 1 minute of exposure.

Correlation between Enterococcus faecalis and Persistent Intraradicular Infection Compared with Primary Intraradicular Infection: A Systematic Review

“…It is reported that 19.4% of gutta-percha cones removed from their packaging contained bacteria on the surface (34); 5.25% sodium hypochlorite and 2% chlorhexidine are the most common disinfectants for gutta-percha cones. Povidone-iodine and peracetic acid have also been studied for rapid disinfection (34)(35)(36)(37).…”

Effect of green tea extract and mouthwash without alcohol on Candida albicans biofilm on acrylic resin

“…Under local and systemic conditions related to the host, it may become virulent and responsible for oral diseases known as candidosis. Candida albicans was the species chosen for this study, due to its strong association with denture stomatitis and other forms of oral candidosis, due to its capacity to adhere to and proliferate in hard and soft tissues, to survive in a complete and heterogeneous microbial biofilm and to show resistance to several therapies …”

“…Afterwards, the specimens were contaminated in vitro by immersion in 10 ml of Sabouraud broth inoculated with 0.1 ml of the standardised suspension. The specimens were incubated at 37°C for 24 h, in the vertical position, for biofilm formation …”

“…The specimens were incubated at 37°C for 24 h, in the vertical position, for biofilm formation. 4,5 Evaluation of the effect of aqueous extract of Camellia sinensis and Listerine â Zero TM The aqueous extract of Camellia sinensis and the oral antiseptic Listerine â Zero TM (LZ), without ethanol (Johnson & Johnson, São Jos e dos Campos, Brazil), were evaluated. Dehydrated Camellia sinensis leaves (Yamamotoyama â , São Miguel Arcanjo, Brazil) were obtained commercially.…”

Disinfection and surface changes of gutta‐percha cones after immersion in sodium hypochlorite solution containing surfactant

“…Several methods and chemical substances may be used for rapid/fast disinfection of gutta‐percha cones, such as 1 and 2% peracetic acid (Salvia, Teodoro, Balducci, Koga‐ito, & Oliveira, ; Subha et al, ); polyvinylpyrrolidone‐iodine (PVPI) (Nebeshima et al, ); 2% chlorhexidine gluconate solution (Gomes et al, ; Nebeshima et al, ); 70% ethyl alcohol, 16% hydrogen peroxide, quaternary ammonia, and finally, the sodium hypochlorite (NaOCl) solution (Gomes et al, ).…”