Effectiveness of a neutral electrolysed oxidising water (NEOW) device in reducing Legionella pneumophila in a water distribution system: A comparison between culture, qPCR and PMA-qPCR detection methods

Bonetta, Sara; Pignata, Cristina; Bonetta, Silvia; Meucci, Lorenza; Giacosa, D.; Marino, E; Gorrasi, Ilaria Silvia Rossella; Gilli, Giorgio; Carraro, Elisabetta

doi:10.1016/j.chemosphere.2018.07.053

Cited by 9 publications

(13 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nowadays, the prevention of Legionella dissemination has an important role in the control of its proliferation in water systems and many chemical disinfection methods have been proposed (Bonetta et al, 2018; Coniglio et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Metabolic Characterization of Supernatants Produced by Lactobacillus spp. With in vitro Anti-Legionella Activity

et al. 2019

View full text Add to dashboard Cite

Legionella pneumophila is an organism of public health interest for its presence in water supply systems and other humid thermal habitats. In this study, ten cell-free supernatants produced by Lactobacillus strains were evaluated for their ability to inhibit L. pneumophila strains isolated from hot tap water. Production of antimicrobial substances by Lactobacillus strains were assessed by agar well diffusion test on BCYE agar plates pre-inoculated with L. pneumophila. Cell-free supernatants (CFS) showed antimicrobial activity against all Legionella strains tested: L. rhamnosus and L. salivarius showed the highest activity. By means of a proton-based nuclear magnetic resonance ( 1 H-NMR) spectroscopy, we detected and quantified the Lactobacillus metabolites of these CFSs, so to gain information about which metabolic pathway was likely to be connected to the observed inhibition activity. A panel of metabolites with variations in concentration were revealed, but considerable differences among inter-species were not showed as reported in a similar work by Foschi et al. (2018) . More than fifty molecules belonging mainly to the groups of amino acids, organic acids, monosaccharides, ketones, and alcohols were identified in the metabolome. Significant differences were recorded comparing the metabolites found in the supernatants of strains grown in MRS with glycerol and the same strains grown in MRS without supplements. Indeed, pathway analysis revealed that glycine, serine and threonine, pyruvate, and sulfur metabolic pathways had a higher impact when strains were grown in MRS medium with a supplement such as glycerol. Among the metabolites identified, many were amino acids, suggesting the possible presence of bacteriocins which could be linked to the anti- Legionella activity shown by cell-free supernatants.

show abstract

Section: Discussionmentioning

confidence: 99%

Metabolic Characterization of Supernatants Produced by Lactobacillus spp. With in vitro Anti-Legionella Activity

et al. 2019

View full text Add to dashboard Cite

show abstract

“…These are all critical aspects related to Legionella persistence in man-made systems that contribute to a higher or lower legionellosis risk. The studies considered in Table 1 cover different water settings, including cooling towers [55][56][57], premise plumbing from hospitals [51,52,54,[58][59][60][61][62][63][64] and hotels [45,48,[64][65][66][67][68][69], residential potable water systems [46,70,71], and other facilities [72][73][74][75][76][77][78].…”

Section: Main Research Topicsmentioning

confidence: 99%

Legionella and Biofilms—Integrated Surveillance to Bridge Science and Real-Field Demands

2021

View full text Add to dashboard Cite

Legionella is responsible for the life-threatening pneumonia commonly known as Legionnaires’ disease or legionellosis. Legionellosis is known to be preventable if proper measures are put into practice. Despite the efforts to improve preventive approaches, Legionella control remains one of the most challenging issues in the water treatment industry. Legionellosis incidence is on the rise and is expected to keep increasing as global challenges become a reality. This puts great emphasis on prevention, which must be grounded in strengthened Legionella management practices. Herein, an overview of field-based studies (the system as a test rig) is provided to unravel the common roots of research and the main contributions to Legionella’s understanding. The perpetuation of a water-focused monitoring approach and the importance of protozoa and biofilms will then be discussed as bottom-line questions for reliable Legionella real-field surveillance. Finally, an integrated monitoring model is proposed to study and control Legionella in water systems by combining discrete and continuous information about water and biofilm. Although the successful implementation of such a model requires a broader discussion across the scientific community and practitioners, this might be a starting point to build more consistent Legionella management strategies that can effectively mitigate legionellosis risks by reinforcing a pro-active Legionella prevention philosophy.

show abstract

“…A range of physical and chemical disinfection methods have been proposed with the aim of controlling Legionella contamination; however, to date, the most effective procedures have not been defined [6,7]. Therefore, alternative disinfection methods that are effective in controlling the proliferation of Legionella could be useful tools to reduce the risk of the spread of Legionnaires' disease.…”

Section: Introductionmentioning

confidence: 99%

“…However, although the assessment of L. pneumophila in water is typically performed by culture isolation on selective media, it has several limits including the long incubation times and the inability to detect the viable but non-culturable bacteria (VBNC). For this reason, in the last decades, alternative tools for rapid, sensitive, and specific detection of Legionella in water samples have been proposed [7,8]. For the identification of possible sources of contamination/infection, high-resolution genotyping of new isolates (e.g., Sequence Based Typing, Multilocus Variable number of tandem repeats) is needed to correlate environmental with clinical isolates.…”

Section: Introductionmentioning

confidence: 99%

Editorial Comments to the Special Issue: “Legionella Contamination in Water Environment”

Bonetta

Bonetta²

2020

Pathogens

Self Cite

View full text Add to dashboard Cite

show abstract

Effectiveness of a neutral electrolysed oxidising water (NEOW) device in reducing Legionella pneumophila in a water distribution system: A comparison between culture, qPCR and PMA-qPCR detection methods

Cited by 9 publications

References 26 publications

Metabolic Characterization of Supernatants Produced by Lactobacillus spp. With in vitro Anti-Legionella Activity

Metabolic Characterization of Supernatants Produced by Lactobacillus spp. With in vitro Anti-Legionella Activity

Legionella and Biofilms—Integrated Surveillance to Bridge Science and Real-Field Demands

Editorial Comments to the Special Issue: “Legionella Contamination in Water Environment”

Contact Info

Product

Resources

About