Introduction: Central line-associated bloodstream infection (CLABSI) When a central artery is infected with pathogens or viruses, it can be quite serious. Healthcare providers may put a central line into one of the larger veins to give medicines, fluids, or blood products, or draw blood for lab tests that people need over a long period. A CLABSI is a laboratory-confirmed bloodstream infection not found to be present or incubating on admission and the patient has had a central line in place for > =2 calendar days by the day of the event, with that same organism also being RECOGNIZED from one other sterile site. Aim: The study aimed to determine the effectiveness of the health education program or knowledge regarding CLABSI. Methodology: A quasi-experimental pretest one-group and post-test design was adopted. In this situation, the population of the study, and the inclusion, and exclusion criteria were applied. Use of sample size, sampling techniques, validation, and data collection participants Population of the study included all B.Sc. Nursing undergraduate students at Sharda University School of Nursing. Research incurred from May to June 2024. A purposive sampling strategy was used in the selection and determination of the sample size of the study which was 60 students using the independent t-test formula. Result: The study shows that 68.3% of the participants must have a poor level of knowledge. 23.3% had an average level of knowledge 8.3% had a good level of knowledge regarding the pretest, a maximum of 63.3% they were having a good level of knowledge, about 26.7% of them are having an average level of knowledge, 10% of them had a poor level of knowledge regarding it. From the pre-test to the post-test the mean knowledge score has significantly increased, in the pre-test was 9.83 S. D= 4.00 and in the post-test, it iwas 15.65 and S. D= 3.99. The Paired T-test value is -8.85 and the p-value is less than 0.01. Conclusion: According to the findings, nursing students need to have a comprehensive grasp of CLABSI to build an understanding of the illness state and to receive proper treatment to prevent the development of other life-threatening disorders and sepsis.