Effectiveness of a vaccine against red sea bream iridoviral disease in a field trial test

Nakajima, Kazuhiro; Maeno, Yukio; Honda, Akira; Yokoyama, Kenichi; Tooriyama, Tetsurou; Manabe, Sadao

doi:10.3354/dao036073

Cited by 109 publications

(44 citation statements)

References 4 publications

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“…Clearly, the amphibian antibody response is integral to the clearance of ranavirus infections, while the extent to which this particular immune mechanism contributes to the ultimate anti-ranaviral immunity seen in Xenopus adults remains to be determined. These results are consistent with fi ndings with Red seabream iridovirus (RSIV, genus Megalocytivirus ) in which vaccination with inactivated virions protected fi sh from subsequent viral challenge (Caipang et al 2006 ;Nakajima and Kunita 2005 ;Nakajima et al 1999 ) …”

Section: Antibody Responses To Ranavirus Infectionsupporting

confidence: 89%

Ranavirus Host Immunity and Immune Evasion

et al. 2015

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Section: Antibody Responses To Ranavirus Infectionsupporting

confidence: 89%

Ranavirus Host Immunity and Immune Evasion

et al. 2015

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“…The DNA sequences were compared with the GenBank/EMBL databases using BLAST (National Center for Biotechnology Information). Sequences were aligned using Clustal W (40), and then a phylogenetic tree was made with the TreeView program (33). The phylogenetic relationships between species were determined using the neighbor-joining method (36), and the reliability of the neighbor-joining tree was inferred using the bootstrap method (12) with 1,000 replicates.…”

Section: Methodsmentioning

confidence: 99%

Phenotypic Diversity of Infectious Red Sea Bream Iridovirus Isolates from Cultured Fish in Japan

Shinmoto

Taniguchi

Ikawa

et al. 2009

Appl Environ Microbiol

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Megalocytivirus is causing economically serious mass mortality by infecting fish in and around the Pacific region of Asia. The recent emergence of many new iridoviruses has drawn attention to the marked taxonomic variation within this virus family. Most studies of these viruses have not included extensive study of these emergent species. We explored the emergence of red sea bream iridovirus (RSIV) on a fish farm in Japan, and we specifically endeavored to quantify genetic and phenotypic differences between RSIV isolates using in vitro and in vivo methods. The three isolates had identical major capsid protein sequences, and they were closely related to Korean RSIV isolates. In vitro studies revealed that the isolates differed in replication rate, which was determined by real-time quantitative PCR of viral genomes in infected cells and cell culture supernatant, and in cell viability, estimated by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay for infected cells. In vivo studies showed that the isolates exhibit different virulence characteristics: infected red sea bream showed either acute death or subacute death according to infection with different isolates. Significant differences were seen in the antigenicity of isolates by a formalin-inactivated vaccine test. These results revealed that variant characteristics exist in the same phylogenetic location in emergent iridoviruses. We suggest that this strain variation would expand the host range in iridoviral epidemics.

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“…An injectable vaccine using formalin-inactivated RSIV is efficacious in protecting red sea bream, yellowtail, amberjack, kelp grouper, and other species from RSIV infection and has been commercialized (Nakajima et al 1999Caipang et al 2006a). Moreover, a DNA vaccine and an oral vaccine using liposome-entrapped RSIV antigens are also effective (Caipang et al 2006b; T. Miyazaki, unpublished observations).…”

Section: Vaccine Developmentmentioning

confidence: 99%

Family Iridoviridae: Poor Viral Relations No Longer

Chinchar

Hyatt

Miyazaki

et al. 2009

Current Topics in Microbiology and Immunology

182

205

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Members of the family Iridoviridae infect a diverse array of invertebrate and cold-blooded vertebrate hosts and are currently viewed as emerging pathogens of fish and amphibians. Iridovirid replication is unique and involves both nuclear and cytoplasmic compartments, a circularly permuted, terminally redundant genome that, in the case of vertebrate iridoviruses, is also highly methylated, and the efficient shutoff of host macromolecular synthesis. Although initially neglected largely due to the perceived lack of health, environmental, and economic concerns, members of the genus Ranavirus , and the newly recognized genus Megalocytivirus , are rapidly attracting

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Effectiveness of a vaccine against red sea bream iridoviral disease in a field trial test

Abstract: ABSTRACT-Since 1990, red sea bream 11-dov virus (RSIV) has to this virus (authors' unpubl, data). Diseased fish caused high mortalities in the summertime in cultured red sea became lethargic and exhibited severe anemid,

Cited by 109 publications

References 4 publications

Ranavirus Host Immunity and Immune Evasion

Ranavirus Host Immunity and Immune Evasion

Phenotypic Diversity of Infectious Red Sea Bream Iridovirus Isolates from Cultured Fish in Japan

Family Iridoviridae: Poor Viral Relations No Longer

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