Effectiveness of antifungal treatments during artificial incubation of the signal crayfish eggs (Pacifastacus leniusculus Dana. Astacidae)

Melendre, P.M.; Celada, J.D.; Carral, J.M.; Sáez‐Royuela, M.; Aguilera, A.

doi:10.1016/j.aquaculture.2006.02.064

Cited by 34 publications

(43 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As it has been proved with other antifungal tested Melendre et al, 2006;Policar et al, 2006;Sáez-Royuela et al, 2009), artificial incubation of astacid crayfish eggs calls for higher concentrations than those recommended for other species. In the case of copper hydroxide, the lower concentrations tested did not avoid the presence of fungus which were observed after 14-19 days of incubation, resulting in poor survival to stage 2 juveniles (2.5% at 80 ppm) or total mortality (at 40 and 60 ppm).…”

Section: Discussionmentioning

confidence: 92%

“…Because their low water solubility, copper complexes, including Cu(OH) 2 , tend to precipitate and thus administration by means of peristaltic pumps does not guarantee the concentration received on eggs, as Melendre et al (2006) pointed out for the copper sulphate. Thus, the application of these chemicals should be restricted to static baths leading to additional man work throughout the AI period.…”

Section: Discussionmentioning

confidence: 99%

“…For many years malachite green has been the most effective fungicide used in aquaculture but since 1991 and 1997 is not permitted for aquaculture in USA (Schereier et al, 1996) and EU respectively, because of its teratogenic and carcinogenic effects (Andersen et al, 2006;Sapkota et al, 2008). After a series of trials testing chemicals used in other aquatic species, Celada et al (2004) and Melendre et al (2006) reported formaldehyde as the most effective antifungal. Although formaldehyde is approved for use in fish culture in USA and EU, there is concern about user safety because of its suspected carcinogenicity and its potential adverse effects on the aquatic environment (Arndt et al, 2001;Gieseker et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…and other Oomycetes (Edgerton et al, 2002). Their hyphae can spread to the surrounding healthy ones Melendre et al, 2006;Sáez-Royuela et al, 2009) causing an important decrease of efficiency rates. Periodical removal of dead eggs has shown positive effect on the hatching rate Policar et al, 2006) but it is laborious and manipulation can cause damages in healthy ones, giving rise to new losses .…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Antifungal treatments in artificial incubation of crayfish eggs (Pacifastacus leniusculus, Astacidae): Searching for alternatives to formaldehyde

Carral

González

Celada

et al. 2009

Knowl. Managt. Aquatic Ecosyst.

Self Cite

View full text Add to dashboard Cite

Key-words:antifungal treatment, artificial incubation, astacid crayfishConsidering the concerns about the safety of the use of formaldehyde as antifungal agent, the effects of three alternative chemicals (potassium sorbate, copper hydroxide and magnesium chloride) were tested in the artificial incubation of signal crayfish (Pacifastacus leniusculus) eggs. Eight treatments were performed during 15 min every other day: formaldehyde at 3000 ppm (control), potassium sorbate at 5000 and 10 000 ppm, copper hydroxide at 40, 60, 80 and 200 ppm, and magnesium chloride at 10 000 ppm. Eggs were incubated in a flow through system at a density of 20 eggs·cm −2 . After 61 days of incubation, the highest efficiencies were obtained with 200 ppm of copper hydroxide (77.6% of survivors to stage 2) with no significant differences from the control (74.3%). Lower concentrations of copper hydroxide resulted in high egg mortality whereas potassium sorbate and magnesium chloride were ineffective to avoid fungal growth and total egg mortality took place. From the obtained results, copper hydroxide baths at 200 ppm could be considered as a good alternative to formaldehyde. fongique et une mortalité totale des oeufs intervenait. À partir des résultats obtenus, des bains d'hydroxyde de cuivre à 200 ppm peuvent être considérés comme une bonne alternative au formaldéhyde.

show abstract

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antifungal treatments in artificial incubation of crayfish eggs (Pacifastacus leniusculus, Astacidae): Searching for alternatives to formaldehyde

Carral

González

Celada

et al. 2009

Knowl. Managt. Aquatic Ecosyst.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Consequently, removal of dead eggs leads to a higher hatching rate and higher juvenile survival. A variety of antifungal agents (formaldehyde, malachite green, hydrogen peroxide, isopropyl alcohol, copper sulphate, potassium permanganate and iodine) have already been tested on astacid crayfish eggs, but proved inefficient for regular use due do toxic characteristics for humans and/or eggs (formaldehyde, potassium permanganate, malachite green, iodine) (Meinertz et al 1995;Celada et al 2004;Sáez-Royuela et al 2009) or showed no antifungal effects (isopropyl alcohol, copper sulphate, hydrogen peroxide) (Celada et al 2004;Melendre et al 2006). Formaldehyde showed good effectiveness at high concentrations (Sáez-Royuela et al 2009) but would not be an option due to its reported harmful reproductive and developmental toxicities, including chromosome and DNA damage (Duong et al 2011) especially under eight degrees Celsius.…”

Section: Introductionmentioning

confidence: 99%

Survival of Early Stripped Eggs of the Noble Crayfish, <i>Astacus astacu</i>, and Effects of Saline Solution During Artificial Incubation

Seemann

Lorkowski

Schiffer

et al. 2014

Freshwater Crayfish

View full text Add to dashboard Cite

There is growing interest in using recirculating aquaculture systems (RAS) to produce juvenile noble crayfish, Astacus astacus (Linnaeus), a valuable and once plentiful food species in Europe, now a highly endangered species. The survival rates of early stripped eggs of A. astacus were compared across anti-fungal treatments in an artificial RAS incubation system based on a saline bath of approx. 20 -22 mS cm -1 or 15 -16 PSU over two different durations. Time from fertilisation to hatching was 82 days or 1191 degreedays. Low survival rates (live successfully hatched juveniles at end of experimental period) between 11.0 ± 9.5% and 26.6 ± 3.7% were obtained. Survival was significantly affected by the duration of egg bath within the saline solution with twice as high survival rates when eggs were saline-bathed once every two days for five minutes in comparison to eggs without treatment. Fungal rates were significantly affected by treating the eggs with a saline solution with infection rates decreasing from 3.7 ± 1.0% to 0.8 ± 0.5% in the ten minute saline bath treatment. Appropriately applied saline bathing can markedly improve survival of early-stripped noble crayfish eggs. However, further research is required to determine whether viable levels of hatching success can be obtained using early stripping and saline treatment.

show abstract

Investigation of hatching and early post‐embryonic life of freshwater crayfish by in vitro culture, behavioral analysis, and light and electron microscopy

Vogt

2008

Journal of Morphology

View full text Add to dashboard Cite

The late embryonic and early post-embryonic life period of freshwater crayfish, which is the main time period of organogenesis, is poorly investigated because of the protective brooding behavior of crayfish mothers. A combination of in vitro culture, behavioral observations, and microscopic investigations of organs involved in hatching, attachment, exploration of the environment, and searching and processing of food yielded deeper insights in this important period of life. Experiments were performed with the robust parthenogenetic marbled crayfish. The following results were obtained: (1) Marbled crayfish can be raised in simple in vitro systems from 80% embryonic development to juvenile Stage 4 with up to 100% survival; (2) Hatching is prepared by chemical weakening of the egg shell and completed by levering actions of the hatchling's appendages; (3) The telson thread, a safety line that keeps the hatchling secured to the mother, is formed by secretions from the telson and the detaching inner layer of the egg case; (4) Molting Stage-1 juveniles are secured by an anal thread that results from delayed molting of the hindgut; (5) Active attachment of the hatchlings to the maternal pleopods with their 1st pereiopods is achieved by an innate fixed action pattern; (6) In vitro, juveniles are motile from Stage 2 despite incomplete development of their balance controlling statocysts. Movement pattern and social behavior vary greatly among individuals; and (7) Feeding starts in Stage 3, when the mouthparts and the gastric mill are fully developed. Onset of feeding is innate and does not require maternal contributions. In vitro culture of the isogenic marbled crayfish is recommended for broader use in research because it enables not only time and stage-specific sampling but also precisely timed experimental manipulations.

show abstract

Effectiveness of antifungal treatments during artificial incubation of the signal crayfish eggs (Pacifastacus leniusculus Dana. Astacidae)

Cited by 34 publications

References 27 publications

Antifungal treatments in artificial incubation of crayfish eggs (Pacifastacus leniusculus, Astacidae): Searching for alternatives to formaldehyde

Antifungal treatments in artificial incubation of crayfish eggs (Pacifastacus leniusculus, Astacidae): Searching for alternatives to formaldehyde

Survival of Early Stripped Eggs of the Noble Crayfish, <i>Astacus astacu</i>, and Effects of Saline Solution During Artificial Incubation

Investigation of hatching and early post‐embryonic life of freshwater crayfish by in vitro culture, behavioral analysis, and light and electron microscopy

Contact Info

Product

Resources

About