Effectiveness of antimicrobial photodynamic therapy mediated by butyl toluidine blue in preventing medication-related osteonecrosis of the jaws in rats

Shedding light on the therapeutic efficacy of oxygen-releasing gel and photodynamic therapy in the repair of periodontal tissue. A study in rats

“…The formation of reactive oxygen species in periodontal tissues, like singlet oxygen can reduced the levels of periodontopathogenic bacteria [19] . In this study we employed the same parameters regarding the BM dosage and the PDT and photosensitizer that was used in our previous pre-clinical and clinical studies [17,27,33] . Thus, the parameters utilized can be translated to a clinical scenario to treat periodontitis.…”

“…Seven days after EP induction, the ligatures were removed [15] and the animals were randomly distributed in six groups (n=18), following a computer generated table, according to the following treatments: EP: without local treatment; SRP group: Scaling and Root Planing followed by local irrigation with physiological saline solution (0.9%); SRP+BM group: SRP followed by local administration of Blue®M gel (Blue®M Europe BV Groteweg, JV Wapenveld, Netherlands; 0.4 mL; 180s); SRP+PDT group: SRP followed by local administration of butyl toluidine blue (0.5 mg/mL) and low power diode laser irradiation (660 nm, 40 mW, 60 s); BM+PDT group: SRP followed by local administration of Blue®M and PDT (Figure 1). The chosen concentration of the photosensitizer butyl toluidine blue was based on previous literature [17,33] .…”