Effectiveness of Bifidobacterium bifidum in Mediating the Clinical Course of Murine Rotavirus Diarrhea

Duffy, Linda C.; Zielezny, Maria; Riepenhoff-Talty, M; Dryja, Diane; Sayahtaheri-Altaie, Sousan; Griffiths, Elizabeth A.; Ruffin, Debra C.; Barrett, Helen J.; Rossman, Jon E.; Ogra, P. L.

doi:10.1203/00006450-199406000-00014

Cited by 75 publications

(33 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In comparison, the viral shedding continued to increase gradually until 7 d PI, the last time point tested in all infected groups. This virus shedding pattern was different from that observed in a previous study with murine rotavirus (MRV) (17), in which the viral shedding in stool declined on d 7 PI in both bifidobacteria and nonbifidobacteria-treated, MRVinfected mice. One possible reason for this difference could be the fact that RRV has liver tropism (19).…”

Section: Discussioncontrasting

confidence: 99%

“…Although bifidobacteria were not routinely isolated in samples from treated mice in these experiments, we cannot summarily conclude that the organisms were not present in the gut, inasmuch as our previous research has shown that B. bifidum can successfully colonize the mouse intestine (17). In that study, 80% of minced small intestine samples from B. bifidumtreated mice were culture positive for B. bifidum, although the ingested strain was not isolated from the intestines of mice challenged with MRV alone or from saline control animals.…”

Section: Discussionmentioning

confidence: 78%

“…Intensive scientific investigations have shown that certain lactic acid bacteria, including bifidobacteria, can reduce the severity of rotavirus infections in humans (14,15) and animals (16,17). However, the combined use of probiotics and prebiotics in the treatment of diarrhea caused by rotavirus has not been studied.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Immune Responses in Rhesus Rotavirus-Challenged Balb/c Mice Treated with Bifidobacteria and Prebiotic Supplements

QIAO¹

2002

Pediatric Research

View full text Add to dashboard Cite

Bifidobacterium species (B. bifidum and B. infantis), with or without prebiotic compounds (arabino-galactan, short-chain fructo-oligosaccharide, iso-malto-dextrins), were orally fed to Balb/c pups (n ϭ 192) to evaluate their potential synergistic effects on modulating the course of rhesus rotavirus (RRV) infection, as well as their ability to mediate the associated mucosal and humoral immune responses. Rotavirus-specific IgA and IgG in serum, rotavirus antigen, and specific IgA in feces were measured by ELISA. Mucosal total IgA and IgG levels were determined in Peyer's patches by flow cytometry. Significantly delayed onset (p ϭ 0.001) and early resolution (p Ͻ 0.001) of diarrhea were observed in bifidobacteria-treated, RRVinfected mice compared with RRV-infected control mice. Supplementation with prebiotic compounds did not shorten the clinical diarrhea course more than that observed with bifidobacteria treatment alone. Rotavirus-specific IgA in feces was 16-fold elevated on d 5 postinfection in bifidobacteria-treated, RRVinfected mice compared with the RRV-infected alone group. In addition, the level of rotavirus-specific IgA in serum was fourfold higher in bifidobacteria-treated, RRV-infected litters versus mice challenged with RRV alone on 28 and 42 d postinfection.No enhancement of the immune response was found in RRVinfected mice that were treated with both bifidobacteria and prebiotic compounds over those treated with bifidobacteria only. The findings suggest that bifidobacteria may act as an adjuvant by modulating early mucosal and strong humoral rotavirusspecific immune responses, and mitigate severity of rotavirusinduced diarrhea. Bifidobacterium species and other lactic acid-producing Gram-positive anaerobes dominate the human colonic microflora, and collectively belong to a group of bacteria defined as probiotics (1). These bacteria are considered to be beneficial to human health through their effects in the gastrointestinal tract (1-3). Health effects attributed to probiotics include immune regulation, cholesterol lowering, prevention of cancer recurrence, and amelioration of diarrhea (4 -6). Because of their potential benefits to human health, various fermented dairy products (capsules, powders, enriched yogurts and milks) containing bifidobacteria and lactobacilli are commercially available.Prebiotics are nondigestible food ingredients that can beneficially affect the host by selectively stimulating the growth and/or activity of certain bacteria in the colon that have positive effects on gut physiology (7). Many oligosaccharides are classified as prebiotics. Oligosaccharides are thought to be assembled by glycosyltransferases, which synthesize cell surface glycoconjungates that are often used as receptors by pathogens (8). Some milk oligosaccharides have been documented to protect the nursing infant by acting as receptor homologs, inhibiting the binding of pathogens to their host receptors, e.g. enteropathogenic Escherichia coli and Campylobacter jejuni (9, 10). In addition, several studies have de...

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 78%

See 1 more Smart Citation

Immune Responses in Rhesus Rotavirus-Challenged Balb/c Mice Treated with Bifidobacteria and Prebiotic Supplements

QIAO¹

2002

Pediatric Research

View full text Add to dashboard Cite

show abstract

“…In conclusion, inhibitory effect of cPGN , live bifidobacterial cell, and their mixture in all concentrations on rotavirus infection was superior when cPGN and live bifidobacterial cell were used simultaneously. Probiotic lactic acid bacteria was well known to protect infection as they competitively acted against adherence to adherent site in epithelial cell (4,17 …”

Section: Variation Of Adherence Ability By Crude Peptidoglycan Dependmentioning

confidence: 99%

Effects of Bifidobacteria-Derived Crude Peptidoglycan on Protection against E.coli O157: H7 and Rotavirus Infection

Kim

Jung

Chun

et al. 2002

Bioscience Microflora

View full text Add to dashboard Cite

“…2) Some Bifidobacterium species are widely used for the preparation of fermented milk products all over the world, and are believed to have health-promoting properties for their host, involving enhancement of immune response, 3) an inhibitory effect for carcinogenesis, 4) protection of the host against viral infection, 5,6) and possible production of antibacterial substances. 7) Furthermore, Bifidobacterial plasmids are expected to be vectors for probiotics and for oral vaccine against infectious diseases.…”

mentioning

confidence: 99%

Structural and Functional Analysis of pTB6 from Bifidobacterium longum

Tanaka

Samura

Kano

2005

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

The complete nucleotide sequence for pTB6 (3,624 bp) from Bifidobacterium longum was determined. This plasmid is 95% homologous in nucleotide (nuc) sequence, and also 92% in RepB aa sequence, to rolling circle replication (RCR) plasmids pKJ36 and pB44, suggesting that pTB6 replicates by the rolling circle mechanism. The putative MembB, MobA, and protein encoding from orf (Orf) I detected were nonessential for plasmid replication. We constructed an immobile shuttle vector from pTB6 and pUC18, which transformed B. longum with a high efficiency of 2:5 Â 10 6 transformants/g DNA.Key words: Bifidobacterium longum; nucleotide sequence of pTB6; RepB and oriV; immobile plasmid; high-efficient transformation Bifidobacteria are Gram-positive anaerobic bacteria, the genome of which is GC-rich.1) These microorganisms are nonpathogenic, and constitute a major part of the normal microflora in the large intestine of humans and some other animals.2) Some Bifidobacterium species are widely used for the preparation of fermented milk products all over the world, and are believed to have health-promoting properties for their host, involving enhancement of immune response, 3) an inhibitory effect for carcinogenesis, 4) protection of the host against viral infection, 5,6) and possible production of antibacterial substances.7) Furthermore, Bifidobacterial plasmids are expected to be vectors for probiotics and for oral vaccine against infectious diseases. A recent report found that B. longum accumulated in hypoxic solid tumors after systemic administration, 8,9) and that a recombinant plasmid, pBLES100-S-eCD, carried E. coli codA fused to B. longum hup promoter, and expressed the cytosine deaminase gene (codA) of E. coli in the microorganism, 10) confirming that the recombinant B. longum is effective for enzyme-prodrug therapy.11) The plasmid vector pBLES100 consisted of pTB6 from B. longum BK51 and pBR322 from E. coli, 12) and it has been reported that the transformation efficiency of B. longum is of the order of 10,000 transformants/mg DNA. 12)Much higher efficiency of transformation, however, is desired for the cloning of foreign genes, because plasmids carrying unmodified DNA might be restricted in several species of Bifidobacteria. It is also necessary that immobile plasmids are applied to vectors to serve as a safety vehicle for gene delivery.The complete nuc sequence (3,624 bp) of pTB6 was determined (the GenBank accession number for the plasmid DNA sequence is AB187597). A database search predicted four Orfs in pTB6 (Fig. 1a), RepB (872 to 1,564), MembB (1,543 to 2,355), MobA (3,300 to 2,212) and a hypothetical protein Orf I (3,587 to 1 to 517). The GC content of this plasmid was 65.1 mol%, and the nuc sequence was 95% homologous with B. longum plasmids pKJ36 and pB44, 92% with pNAC2 and pDOJH10L (nuc position 5,308 to 8,999) and 89% with pBLO1, which belong to the RCR-type plasmid family group 1. 13) A nucleotide sequence similar to oriV was detected between nuc positions 305 and 664 (Fig. 1, b), while, IR (481 to 504) and DR...

show abstract

Effectiveness of Bifidobacterium bifidum in Mediating the Clinical Course of Murine Rotavirus Diarrhea

Cited by 75 publications

References 6 publications

Immune Responses in Rhesus Rotavirus-Challenged Balb/c Mice Treated with Bifidobacteria and Prebiotic Supplements

Immune Responses in Rhesus Rotavirus-Challenged Balb/c Mice Treated with Bifidobacteria and Prebiotic Supplements

Effects of Bifidobacteria-Derived Crude Peptidoglycan on Protection against E.coli O157: H7 and Rotavirus Infection

Structural and Functional Analysis of pTB6 from Bifidobacterium longum

Contact Info

Product

Resources

About