Effectiveness of Bioactive Food Components in Experimental Colon Carcinogenesis

Hijová, Emília; Chmelařová, Anna; Bomba, Alojz

doi:10.2754/avb200978040661

Cited by 7 publications

(2 citation statements)

References 26 publications

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“…SCFA supplement was prepared in serum‐free MEM by mixing 15 μ m of sodium acetate, 5 μ m of sodium propionate, and 5 μ m of sodium butyrate (3:1:1 ratio), mimicking human physiological concentrations and ratios in the descending colon. [ 58,59 ] Viable LGG in MRS broth were harvested at the mid‐log phase (7.5 h after inoculation), quantified, and diluted to 1 x 10 8 CFU mL −1 for treatment. Caco‐2 cells were seeded in six well plates at 1.3 × 10 6 cells per well.…”

Section: Methodsmentioning

confidence: 99%

Lactobacillus rhamnosus GG and Oat Beta‐Glucan Regulated Fatty Acid Profiles along the Gut‐Liver‐Brain Axis of Mice Fed with High Fat Diet and Demonstrated Antioxidant and Anti‐Inflammatory Potentials

Yau

El-Nezami

Galano

et al. 2020

Molecular Nutrition Food Res

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Scope This study takes a novel approach to investigate the anti‐inflammatory and antioxidant effects of prebiotic oat beta‐glucan (OAT) and the probiotic Lactobacillus rhamnosus GG (LGG) against high‐fat diets (HFD) by examining the fatty acid profiles in the gut‐liver‐brain axis. Method and Results HFD‐fed C57BL/6N mice are supplemented with OAT and/or LGG for 17 weeks. Thereafter, mass spectrometry‐based targeted lipidomics is employed to quantify short‐chain fatty acids (SCFA), polyunsaturated fatty acids (PUFA), and oxidized PUFA products in the tissues. Acetate levels are suppressed by HFD in all tissues but reversed in the brain and liver by supplementation with LGG, OAT, or LGG + OAT, and in cecum content by LGG. The n‐6/n‐3 polyunsaturated fatty acid (PUFA) ratio is elevated by HFD in all tissues but is lowered by LGG and OAT in the cecum and the brain, and by LGG + OAT in the brain, suggesting the anti‐inflammatory property of LGG and OAT. LGG and OAT synergistically, but not individually attenuate the increase in non‐enzymatic oxidized products, indicating their synbiotic antioxidant property. Conclusion The regulation of the fatty acid profiles by LGG and OAT, although incomplete, but demonstrates their anti‐inflammatory and antioxidant potentials in the gut‐liver‐brain axis against HFD.

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Section: Methodsmentioning

confidence: 99%

Lactobacillus rhamnosus GG and Oat Beta‐Glucan Regulated Fatty Acid Profiles along the Gut‐Liver‐Brain Axis of Mice Fed with High Fat Diet and Demonstrated Antioxidant and Anti‐Inflammatory Potentials

Yau

El-Nezami

Galano

et al. 2020

Molecular Nutrition Food Res

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“…Modifications in the concentrations or the ability of host tissues to use SCFAs have been described broadly . Moreover, measurement of SCFAs in biological samples has received increasing attention due to the important role that SCFAs play in physiological and pathological processes .…”

Section: Characterizing Metabolic Interactions Between Microbe and Hostmentioning

confidence: 99%

Metabolomics approaches for characterizing metabolic interactions between host and its commensal microbes

et al. 2013

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It is increasingly evident that the gut microbiota is involved in the regulation of multiple mammalian metabolic pathways through a series of interactive host-microbiota metabolic, signaling, and immune-inflammatory axes that physiologically connect the gut, liver, brain, and other organs. Correlation of the metabotypes with the gut microbial profiles derived from culture-independent molecular techniques is increasingly useful for deciphering inherent and intimate host-microbe relationships. Real-time analysis of the small molecule metabolites derived from gut microbial-host co-metabolism is essential for understanding the metabolic functions of the gut microbiome and has tremendous implications for personalized healthcare strategies. Metabolomics, an array of analytical techniques that includes high resolution NMR spectroscopy and chromatography-MS in conjunction with chemometrics and bioinformatics tools, enables characterization of the metabolic footprints of mammalian hosts that correlate with the microbial community in the intestinal tract. The metabolomics approach provides important information of a complete spectrum of metabolites produced from the gut microbial-mammalian co-metabolism and is improving our understanding of the molecular mechanisms underlying multilevel host-microbe interactions. In this review, the interactions of gut microbiota with their host are discussed and some examples of NMR- or MS-based metabolomics applications for characterizing the metabolic footprints of gut microbial-host co-metabolism are described. Advances in the metabolomic analysis of bile acids, short-chain fatty acids, and choline metabolism are also summarized.

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Intestinal-Based Diseases and Peripheral Infection Risk Associated with Gut Dysbiosis: Therapeutic use of Pre- and Probiotics and Fecal Microbiota Transplantation

Tungland¹

2018

Human Microbiota in Health and Disease

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Effectiveness of Bioactive Food Components in Experimental Colon Carcinogenesis

Cited by 7 publications

References 26 publications

Lactobacillus rhamnosus GG and Oat Beta‐Glucan Regulated Fatty Acid Profiles along the Gut‐Liver‐Brain Axis of Mice Fed with High Fat Diet and Demonstrated Antioxidant and Anti‐Inflammatory Potentials

Lactobacillus rhamnosus GG and Oat Beta‐Glucan Regulated Fatty Acid Profiles along the Gut‐Liver‐Brain Axis of Mice Fed with High Fat Diet and Demonstrated Antioxidant and Anti‐Inflammatory Potentials

Metabolomics approaches for characterizing metabolic interactions between host and its commensal microbes

Intestinal-Based Diseases and Peripheral Infection Risk Associated with Gut Dysbiosis: Therapeutic use of Pre- and Probiotics and Fecal Microbiota Transplantation

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