Effectiveness of different auxiliary chemical substances in the rapid disinfection of gutta-percha points - an in vitro study

Schmidt, Maico Henrique Manica; Sallenave, Rafaela Flores; Demarchi, Aline; Farina, Ana Paula; Cecchin, Doglas; Souza, Matheus Albino

doi:10.5335/rfo.v20i1.4614

Cited by 3 publications

(4 citation statements)

References 30 publications

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“…Antes do uso dos cones de guta-percha, indica-se a desinfecção desses materiais, uma vez que podem estar contaminados na embalagem 12 ou contaminar-se durante manuseio ou por aerossóis da clínica odontológica 1,2,4,6,[16][17][18][19][20][21][22][23][24] .…”

Section: Discussionunclassified

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Estudo in vitro sobre a efetividade de desinfetantes na limpeza de cones de guta-percha

Birck

Miyagaki

Corralo

et al. 2016

Rev. da Fac. de Odontologia, UPF

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Introdução: para se obter um tratamento endodôntico de sucesso, várias etapas devem ser seguidas. Igual-mente, um rigoroso esquema para máxima redução dos microrganismos presentes deve ser seguido bem como para sua não introdução. O material obturador de canais mais utilizado mundialmente, a guta-percha, por não poder ser esterilizada, deve ser desinfetada para que não haja a recontaminação do conduto radicular. Objetivo: avaliar a capacidade biocida de três soluções: hipoclorito de sódio a 5,25%, clorexidina a 2% e ácido peracético a 0,2%, em um período de tempo de um minuto, em cones de guta-percha previamente contaminados com Enterococcus faecalis. Materiais e método: foram utilizados oitenta cones, divididos em quatro grupos igualmente (n = 10). Em cada grupo, foi utilizada uma solução desinfetante, exceto no grupo um, no qual foi realizada apenas uma lavagem com água destilada estéril. Resultados: os resultados dessa avaliação indicaram que as soluções de ácido peracético e clorexidina foram 100% eficazes, enquanto que o hipoclorito alcançou 95% de efetividade. A água destilada foi completamente ineficaz. Conclusão: todos os desinfetantes testados nesta pesquisa foram efetivos na descontaminação rápida de cones de guta-percha.

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Section: Discussionunclassified

“…O terceiro desinfetante utilizado, o hipoclorito, que apresentou crescimento bacteriano no presente estudo, obteve 95% de efetividade. Os trabalhos realizados anteriormente demonstraram que o hi-poclorito foi efetivo 1,24,34,36,38,39 . Alguns estudos não encontraram diferenças na descontaminação entre o hipoclorito e a clorexidina 4,23,40 .…”

Section: Discussionunclassified

Estudo in vitro sobre a efetividade de desinfetantes na limpeza de cones de guta-percha

Birck

Miyagaki

Corralo

et al. 2016

Rev. da Fac. de Odontologia, UPF

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“…Such findings are contrary to those found in the literature, whose results showed disinfection only within one minute 18,20 . However, some authors have shown that the time of 30 seconds is sufficient 21 , while other studies have shown that 2% chlorhexidine is only effective within 10 minutes 14 .…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of disinfecting substances of gutta-percha cones

et al. 2022

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Aim: To demonstrate the effectiveness of disinfecting substances with 2% and 5% Sodium Hypochlorite and 2% Chlorhexidine Gluconate at each of the pre-established times of 0:15 and 0:30 seconds, and 1, 2, 5, and 10 minutes. Methods: This study selected 96 gutta-percha cones that were contaminated with Enterococcus Faecalis, dried and treated with the aforementioned substances and applied at pre-established times. Subsequently, these were transferred to sterile Brain Heart Infusion broth and placed in a bacteriological incubator at 37°C for 24 hours to evaluate microbial growth, as well as in a nutrient agar medium in Petri dishes. Half of the cone was transferred to individual filter paper packages and exposed to the environment in a dental clinic at Universidade José do Rosário Velano, for 7 days, with subsequent evaluation for microbial growth. The bacterial phenotype test was performed using Gram stain and growth in 6.5% saline solution. The results were submitted to statistical analysis using the Kruskal Wallis H test, with a significance level of 5%. Results: The substances were effective at all times tested and individual storage supported disinfection. In the statistics test, the p-value was greater than 0.05, as there was no variability in the data configurations. Conclusion: The disinfection of gutta-percha cones and individual storage was an effective protocol to be adopted with 2% and 5% Sodium Hypochlorite and 2% Chlorhexidine.

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“…Guttapercha cones cannot withstand sterilization processes, so they must undergo a decontamination step to ensure the maintenance of the aseptic chain. Although several chemical agents have been tested, there is no consensus in the literature on which is the best protocol to disinfect these materials 2,3,12,13 . Under these circumstances, the use of NaOCl for rapid decontamination of guttapercha cones was proposed by Senia et al 14 , who demonstrated that when contaminated with the bacteria Staphylococcusepidermidis, Corynebacteriumxerosis, Escherichia coli and Enterococcus faecalis, they were decontaminated after immersion in Clorox ® (5.25% sodium hypochlorite) for 30, 45 and 60 seconds.…”

Section: Introductionmentioning

confidence: 99%

Decontamination of Gutta-percha Cones employed in Endodontics.

Carvalho

Pinto

Batista

et al. 2020

Acta Odontol Latinoam

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The gutta-percha cones used in endodontic treatment are produced in aseptic conditions and their composition includes zinc oxide, which is responsible for antibacterial activity. However, there is the possibility of microbial contamination by manipulation, aerosol or during storage. Although several chemical agents have been tested for their decontamination, there is no consensus on the best disinfection protocol to be used. The aim of this study was to evaluate the decontamination of gutta-percha cones contaminated with the bacteria Enterococcus faecalis, by using chlorhexidine digluconate (CHX) and sodium hypochlorite (NaClO) at different concentrations for short exposure times. For this purpose, gutta-percha cones (size 40) were selected at random from a sealed box and immersed for 1 min in a microbial suspension. Then they were immersed in specific Petri dishes for different groups containing: CHX 2%, NaClO 1% or NaClO 2.5% for 30 s or 1 min, and subsequently placed in tubes containing BHI broth. After incubating the tubes for 48 h, it was observed that 1% and 2.5% NaClO and 2% CHX were effective for decontaminating the cones at those exposure time intervals. Microbial growth was detected in one of the replicates of the group with CHX applied for 30 s. To prevent the possibility of failures at this stage, the

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Effectiveness of different auxiliary chemical substances in the rapid disinfection of gutta-percha points - an in vitro study

Cited by 3 publications

References 30 publications

Estudo in vitro sobre a efetividade de desinfetantes na limpeza de cones de guta-percha

Estudo in vitro sobre a efetividade de desinfetantes na limpeza de cones de guta-percha

Effectiveness of disinfecting substances of gutta-percha cones

Decontamination of Gutta-percha Cones employed in Endodontics.

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