With the objective of determining the production, quality and development of bovine embryos (Bos Indicus), oocytes were collected from 14 females distributed in two groups: empty females (T1; n=7) and an experimental group of pregnant females (T2; n=7). The two groups were subjected to Ovum Pick Up (OPU) using disposable 18-gauge needles and a vacuum pressure of 100 mmHg. to later carry out in vitro fertilization (IVF). The study variables were the number of oocyte aspirated and fertilized and the expected embryos. A Wilcoxon test was performed for non-parametric data between two groups, finding no significant differences between treatments P> 0.05. 250 oocytes were recovered by fertilizing out of 190, obtaining a fertilization rate of 77%, reaching a transferrable blastocyst rate of 24% (45/190). In empty females (7), 113 oocytes were obtained, reaching a fertilization rate of 75% (81/113) and 25% transferable blastocysts (18/81). In the 7 pregnant females, 137 oocytes were collected, reaching a fertilization rate of 83% (109/137) and 23% transferable blastocysts (27/109). Follicular aspiration for in vitro fertilization of embryos from pregnant and empty cows is viable without differences between them, but being more variable in pregnant cows.