Effectiveness of Ethylenediamine-N(o-hydroxyphenylacetic)-N′(p-hydroxyphenylacetic) acid (o,p-EDDHA) to Supply Iron to Plants

Abstract

The Fe chelate o,p-EDDHA/Fe 3+ , in addition to o,o-EDDHA/Fe 3+ , was found recently to be a component of commercial EDDHA/Fe 3+ chelates. The European Regulation on fertilisers has included o,p-EDDHA as an authorized chelating agent. The efficacy of o,o-EDDHA/Fe 3+ , o,p-EDDHA/Fe 3+ and EDTA/Fe 3+ chelates as Fe sources in plant nutrition was studied. Iron-chelate reductase (FC-R) in young cucumber plants (Cucumis sativus L.) roots reduced o,p-EDDHA/Fe 3+ faster than o,o-EDDHA/ Fe 3+ , EDTA/Fe 3+ and a commercial source of EDDHA/Fe 3+ . The o,p-EDDHA/Fe 3+ chelate was also more effective than the o,o-EDDHA/Fe 3+ in decreasing the severity of Fe-deficiency chlorosis in leaves of young soybean (Glycine max L.) plants grown hydroponically. The o,p-EDDHA ligand was more effective in the short-term than the EDTA and o,o-EDDHA ligands at dissolving Fe from selected Fe minerals and soils. However, the ultimate quantity of dissolve Fe was greatest with the o,o-EDDHA ligand.Abbreviations: BPDS -Bathophenanthroline disulfonic acid or 4,7-diphenyl-1,10-phenantroline disulfonic acid; EDDA -ethylene diamine diacetic acid; EDTA -Ethylene diamine tetraacetic acid; FC-R -Iron chelate reductase; HEPES -4-(2-Hydroxyethyl)piperazine-1

The behaviour of EDDHA isomers in soils as influenced by soil properties

“…Secondly, o,p-EDDHA determines the extent of Cu solubilization. The ability of o,p-EDDHA to dissolve Cu from the soil has been reported by Garcia-Marco et al (2006). For the six treatments in this study containing o,o;40%o,o and 60%o,o;both F and P), o,p-EDDHA was the only isomer of which the initial concentrations were approximately the same (Fig.…”

“…To improve the understanding on the interaction of FeEDDHA isomers with soil and soil constituents, several studies have been done (Alvarez-Fernandez et al 1997Cantera et al 2002;Garcia-Marco et al 2006;Garcia-Mina et al 2003;Hernandez-Apaolaza et al 2006;Hernandez-Apaolaza and Lucena 2001;Siebner-Freibach et al 2004). Soil organic matter (acid peat) and iron (hydr)oxides (ferrihydrite) have been identified as the most reactive, and calcium carbonate and clay (Ca-montmorillonite) as less reactive soil constituents with respect to FeEDDHA sorption (Alvarez-Fernandez et al 1997.…”

Corrección de la clorosis férrica con quelato EDDHA en cultivos sembrados en suelo alcalino y calcáreo

“…La aplicación de FeSO 4 foliar en soya y okra, mantuvo incrementos en la biomasa superiores a los del testigo; mientras que en frijol los incrementos fueron similares al tratamiento quelante. Estos resultados coinciden con los reportados por García-Marco et al (2006) quienes demostraron la efectividad del quelato EDDHA para la corrección de clorosis férrica, asociada a la mayor promoción de la biomasa aérea en plántulas de soya. De igual forma, Kabir et al (2016) registraron en chícharo (Pisum sativum), corrección de clorosis férrica con aspersiones de FeSO 4 e incrementos en la biomasa foliar.…”

“…etilendiamino-di-(o-hidroxifenilacético)], el cual muestra efectividad en suelos calcáreos con pH > 8. 5 (Álvarez et al, 2005;García-Marco et al, 2006). Este quelato se ha utilizado para la corrección de clorosis férrica principalmente en frutales y cultivos hortícolas (Ozores-Hampton, 2013;Whiting et al, 2015); con escasa información de su aplicación en granos o cultivos extensivos.…”

The Effectiveness of FeEDDHA Chelates in Mending and Preventing Iron Chlorosis in Soil-Grown Soybean Plants

“…There is much experience with soybean in Fe chlorosis research; in nutrient solutions, in pot cultures and in the field (e.g. Garcia-Marco et al 2006;Goos et al 2004;Goos and Johnson 2000;Heitholt et al 2003;Wallace and Cha 1986). …”