“…injection of a virus cell culture or homogenate of diseased fish (Jeong et al, 2008a, b;Jun et al, 2009;Kim et al, 2012;Nakajima et al, 1997;Oshima et al, 1998). Jung et al (1997) and Tanaka et al (2003) reported 90 and 75 % mortality of red sea bream by immersion infection with RSIV of 10 2.7 and 10 0.8 TCID 50 ml 21 which was passaged on grunt fin (GF) cells (Clem et al, 1961), respectively. However, since the main purpose of these reports was to study the histopathology of RSIVinfected fish (Jung et al, 1997) and the effectiveness of fasting against red sea bream after infection (Tanaka et al, 2003), they did not detail the procedures for the propagation of RSIV.…”