Effectiveness of gamma ray irradiation and ethyl methane sulphonate on in vitro mutagenesis of strawberry

Murti, Rudi Hari; Kim, Hwa Yeong; Yeoung, Young Rog

doi:10.5897/ajb12.1386

Cited by 20 publications

(5 citation statements)

References 33 publications

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“…Husselman et al (2014) found that increased doses of gamma rays of 100 and 120 Gy were lethal to papaya when treated with dosages ranging from 0 to 120 Gy. The lethal dose of gamma rays for different fruit crops was reported by Surakshitha et al (2017) in grapes and Murti et al (2013) in strawberries.…”

Section: Resultsmentioning

confidence: 98%

Mutagenic effectiveness, efficiency and dose optimization of gamma rays in papaya (Carica papaya L.) varieties

2022

EJPB

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The present study was conducted to determine the mutagenic effectiveness, efficiency and lethal dose of gamma rays in papaya varieties CO7, Arka Prabhath and Arka Surya. Papaya seeds were exposed to various doses of gamma rays (0Gy, 100Gy, 150Gy, 200Gy, 250Gy, 300Gy, 350Gy, 400Gy, 450Gy and 500Gy) and the mutagenic effectiveness and efficiency of gamma rays were assessed based on the chlorophyll mutation frequency and biological damage in M 1 plant basis. There was a negative relationship between mutagenic effectiveness and efficiency with the doses of gamma irradiation. LD 50 value was optimized in CO7, Arka Prabhath and Arka Surya as 354, 348 and 341Gy respectively. Generally, the germination, survival and growth rate of papaya decreased with an increase in gammairradiation doses. Lower doses of mutations created very few mutations and higher doses of gamma irradiation induced lethality and sterility of seedlings whereas, lethal dose 50 has proved to be more effective in inducing desirable mutations in papaya. Since identifying the mutagenic efficiency and effectiveness of the mutagen and optimization of lethal dose is the preliminary step in any mutagenic experiment, this study will offer a foundation for further gamma irradiation studies in papaya to develop desirable mutants.

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Section: Resultsmentioning

confidence: 98%

Mutagenic effectiveness, efficiency and dose optimization of gamma rays in papaya (Carica papaya L.) varieties

2022

EJPB

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“…Employing more than one mutagen results in further improvement in recovery of somaclones in vitro. Murti et al (2013) exposed the strawberry ‘DNKW001’ to the doses of 0, 30, 80, 130, 180, 230, 280, 300 and 325 Gy and similar doses of gamma rays + EMS 7 µM treatments. Their results showed that Gamma ray irradiation + EMS was more effective to generate more type and magnitude of variants.…”

Section: Recovery Of Somaclonal Variantsmentioning

confidence: 99%

Somaclonal variations and their applications in horticultural crops improvement

Krishna¹,

Alizadeh

Singh³

et al. 2016

3 Biotech

343

216

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The advancements made in tissue culture techniques has made it possible to regenerate various horticultural species in vitro as micropropagation protocols for commercial scale multiplication are available for a wide range of crops. Clonal propagation and preservation of elite genotypes, selected for their superior characteristics, require high degree of genetic uniformity amongst the regenerated plants. However, plant tissue culture may generate genetic variability, i.e., somaclonal variations as a result of gene mutation or changes in epigenetic marks. The occurrence of subtle somaclonal variation is a drawback for both in vitro cloning as well as germplasm preservation. Therefore, it is of immense significance to assure the genetic uniformity of in vitro raised plants at an early stage. Several strategies have been followed to ascertain the genetic fidelity of the in vitro raised progenies comprising morpho-physiological, biochemical, cytological and DNA-based molecular markers approaches. Somaclonal variation can pose a serious problem in any micropropagation program, where it is highly desirable to produce true-to-type plant material. On the other hand, somaclonal variation has provided a new and alternative tool to the breeders for obtaining genetic variability relatively rapidly and without sophisticated technology in horticultural crops, which are either difficult to breed or have narrow genetic base. In the present paper, sources of variations induced during tissue culture cycle and strategies to ascertain and confirm genetic fidelity in a variety of in vitro raised plantlets and potential application of variants in horticultural crop improvement are reviewed.

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“…Sahu et al (2019) reported that LD 50 value was 28.35 Gy and 33.13 Gy for pre-soaked seeds & 24.05 Gy and 23.78 Gy for seeds immersed in water for Ranchi local and Arka Surya varieties of papaya respectively. The lethal dose of gamma rays for different fruit crops was reported by Surakshitha et al (2017) in grapes and Murti et al (2013) in strawberries.…”

Section: Resultsmentioning

confidence: 98%

Determination of mutagenic sensitivity and its manifestations on papaya (Carica papaya L.) cv. Arka Prabhath

Bhat,

Dinesh,

Vageeshbabu

et al. 2023

J. Hortic. Sci.

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Papaya is an important fruit crop of the family Caricaceae which needs the improvement in terms of virus resistance and shelf life with dwarf stature. Mutation breeding technique has been considered as an efficient tool adopted by plant breeders to create variability in papaya. The mutation frequency and population structure of the mutants directly depend upon the type of mutagen and the time of exposure. Irrespective of the used mutagens, the ultimate induced mutations are random and therefore determination of mutagenic sensitivity is important pre-requisite. Based on this, investigation on the induction of mutation in papaya cv. Arka Prabhath was carried out with the objective of creating genetic variability through physical mutagen. In this study, papaya seeds were irradiated with five different dose of gamma rays ranging from 50 Gy to 500 Gy. The results revealed that gradual reduction in germination, survival of seedlings and delayed germination with increase in dosage of gamma rays. Based on probit analysis, LD50 (Lethal dose) was fixed at 186.24 Gy. Total seven types of chlorophyll mutants were observed as a result of mutation. Mutagenic efficiency and effectiveness were higher in a lower dose of gamma treatment (50 Gy).

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Effectiveness of gamma ray irradiation and ethyl methane sulphonate on in vitro mutagenesis of strawberry

Cited by 20 publications

References 33 publications

Mutagenic effectiveness, efficiency and dose optimization of gamma rays in papaya (Carica papaya L.) varieties

Mutagenic effectiveness, efficiency and dose optimization of gamma rays in papaya (Carica papaya L.) varieties

Somaclonal variations and their applications in horticultural crops improvement

Determination of mutagenic sensitivity and its manifestations on papaya (Carica papaya L.) cv. Arka Prabhath

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