Effectiveness of high-dose glucocorticoids on hemolysis, elevating liver enzymes, and reducing platelets syndrome

Kang, Suya; Zhou, Liping; Wang, Xiaoyan; Li, Yongmei; Wang, Yun

doi:10.1177/0300060518809783

Cited by 8 publications

(10 citation statements)

References 13 publications

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“…Salmcide-p1 of 100 μl, twofold dilutions from the purified protein stock concentration in 512 μg ml −1 , was added to 100 μl of blood cells and incubated at 37°C for 1 h. 0.1% (v/v) TritonX-100 or PBS was added instead of Salmcide-p1 as a positive and negative haemolytic control, respectively. The absorbance (OD 570 ) of samples was measured after 1 h at 37°C (Kang et al, 2019). The haemolytic activity was calculated according to the equation: Haemolysis rate (%) = (OD measured value − OD negative control)/(OD positive control − OD negative control) × 100%.…”

Section: Antibacterial and Haemolytic Activity In Vitromentioning

confidence: 99%

An endolysin Salmcide-p1 from bacteriophage fmb-p1 against gram-negative bacteria

Meng

Lü

Bie

et al. 2022

Journal of Applied Microbiology

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Aims: A novel endolysin Salmcide-p1 was developed as a promising candidate of new preservative and a supplement to effective enzyme preparations against gramnegative bacterial contaminations.Methods and Results: Salmcide-p1 was identified by complementing the genomic sequence of a virulent Salmonella phage fmb-p1. Salmcide-p1 of 112 μg ml −1 could quickly kill Salmonella incubated with 100 mmol l −1 EDTA, with no haemolytic activity. Meanwhile, Salmcide-p1 had a high activity of lysing Salmonella cell wall peptidoglycan. At different temperatures (4-75°C), pH (4-11) and NaCl concentration (10-200 mmol l −1 ), the relative activity of Salmcide-p1 was above 60%. At 4°C, the combination of Salmcide-p1 and EDTA-2Na could inhibit the number of Salmonella Typhimurium CMCC 50115 in skim milk to less than 4 log CFU ml −1 by 3 days, and the number of Shigella flexneri CMCC 51571 was lower than 4 log CFU ml −1 by 9 days.Conclusions: Salmcide-p1 had a wide bactericidal activity against gram-negative bacteria and showed a broader anti-Salmonella spectrum than the phage fmb-p1.The combination strategy of Salmcide-p1 and EDTA-2Na could significantly inhibit the growth of gram-negative bacteria inoculated in skim milk.Significance and Impact of the Study: Bacteriophage endolysin as an antibacterial agent is considered to be a new strategy against bacterial contamination.

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Section: Antibacterial and Haemolytic Activity In Vitromentioning

confidence: 99%

An endolysin Salmcide-p1 from bacteriophage fmb-p1 against gram-negative bacteria

Meng

Lü

Bie

et al. 2022

Journal of Applied Microbiology

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“…HELLP (hemolysis, elevated liver enzymes, and low platelets) syndrome is a severe form of preeclampsia, which refers to a group of clinical syndromes typically characterized by hemolysis, elevated liver enzymes and low platelets in pregnant women on the basis of gestational hypertension or severe preeclampsia (SPE) and other diseases ( 1 ). Affected patients are often accompanied by epigastric/right upper quadrant pain (40–100% incidence) ( 2 ), hypertension and proteinuria (80–85% incidence) ( 3 ), fatigue, nausea and vomiting, sudden weight gain and headache etc.…”

Section: Introductionmentioning

confidence: 99%

Clinical Classification, Pregnancy Outcomes and Risk Factors Analysis of Severe Preeclampsia Complicated With HELLP Syndrome

et al. 2022

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PurposeTo investigate the clinical classification, pregnancy outcomes and risk factors of pregnant women with severe preeclampsia (SPE) complicated with HELLP (hemolysis, elevated liver enzymes, and low platelets) syndrome.MethodsThe clinical data of 50 pregnant women diagnosed with SPE complicated with HELLP syndrome in our hospital from January 2014 to January 2021 were retrospectively analyzed, and they were selected as the observation group. An additional 50 maternities diagnosed with preeclampsia (PE) during the same period were selected as the control group. The clinical classification and pregnancy outcomes of pregnant women in the observation group were recorded. The age and gestational age of onset of pregnancy were recorded and compared between the two groups. Univariate analysis and multivariate logistic regression model were used to analyze the risk factors for its occurrence.ResultsAmong the 50 maternities in the observation group, there were 10 cases of type I, accounting for 20.00%; 35 cases of type II, accounting for 70.00%; 5 cases of type III, accounting for 10.00%. Partial 33 cases, the composition ratio of 66.00%; complete 17 cases, the composition ratio of 34.00%. Among the fetuses of 50 maternities in the observation group, 35 were premature, accounting for 70.00%; 13 had fetal growth restriction, accounting for 26.00%; and 2 died during perinatal period, accounting for 4.00%. Among the 50 maternities in the observation group, 48 cases were cesarean section, the composition ratio was 96.00%; 2 cases were induced labor, the composition ratio was 4.00%; there was no natural birth, the composition ratio was 0.00%. Univariate analysis showed that age, gestational age at onset, gestational age at termination of pregnancy, HGB, LDH, ALT, AST, TBIL, PLT, PT, and FIB were all associated with the occurrence of SPE complicated with HELLP syndrome (P < 0.05). Multivariate logistic analysis showed that gestational age at onset, gestational age at termination of pregnancy, HGB, LDH, ALT, AST, TBIL, PLT, and FIB were independent risk factors for SPE complicated with HELLP syndrome (P < 0.05).ConclusionSPE complicated with HELLP syndrome has significantly increased adverse pregnancy outcomes. Understanding its clinical classification is of great significance for the preventive application of platelet transfusion therapy and the selection of transfusion timing. Gestational age at onset and gestational age at termination of pregnancy are independent risk factors for its occurrence. Fully understanding the high-risk factors of HELLP syndrome, taking preventive measures in time, and carrying out targeted nursing can effectively improve the prognosis of pregnant women and reduce the risk of HELLP syndrome.

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“…[14], [15] Therapiesincluding glucocorticoids,plasmapheresis, and the transfusion of blood products such as platelets are mainly symptomatic treatments, and their indications and effectiveness have produced conflicting results. [16]- [17] [18][19] [20][21] [22][23]So far, there are no effective targeted measures to halt the progression of HELLP syndrome, let alone etiotropic measures to prevent its occurrence. [24] From a clinical point of view, early diagnosis and timely treatment are of great importancein reducing the adverse outcomes of HELLP syndrome, as well as reducing the risksto pregnant women and perinatal babies.…”

mentioning

confidence: 99%

Expression of Insulin-Like Growth Factor Binding Protein-3 in HELLP Syndrome

Kang

Zhou

2023

Preprint

Self Cite

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Objective To investigate the expression of insulin-like growth factor binding protein-3(IGFBP-3) in HELLP syndrome and its possible role in the pathogenesis of this disease.Methods 1) 87 subjects were enrolled, including 29 patients with HELLP syndrome, 29 patients with simple pre-eclampsia(PE), and 29 healthy gravidae as control. The expression of IGFBP-3, IGF-1, TGF-β1, and VEGF in maternal and umbilical blood of them were detected using ELISA. Correlation analysis was used to observe the correlation between IGFBP-3 and IGF-1/TGF-β1/VEGF in maternal andumbilical blood, as well as that between maternal serum IGFBP-3 and clinical diagnostic indicators of HELLP syndrome. 2)Human hepatic sinusoid endothelial cells (HLSEC) and human umbilical vein endothelial cells (HUVEC) were cultured with different concentrations of IGFBP-3. After 72 hours of culture, cell apoptosis and the normal living cells rate were detected and compared.Results 1)In both maternal and umbilical blood of HELLP group, levels of IGFBP-3 and TGF-β1 were higher than control and PE group, IGF-1was lower than control group, VEGF was lower than control and PE group.IGFBP-3 in maternal blood was correlated with IGF-1/TGF-β1/ VEGF, while IGFBP-3 inumbilical blood was linked to IGF-1/TGF-β1.In maternal blood, there was a negative correlation between PLT and IGFBP-3, and a positive correlation between ALT/AST/LDH and IGFBP-3. 2) After cultured with IGFBP-3, the total apoptosis rate of either HLSEC or HUVEC was considerablyelevated, while the normal living rate was decreased.Conclusion The expression of IGFBP-3 is elevated in HELLP syndrome, which may subsequently promote cell apoptosis by affecting the expression and function of IGF-1, VEGF, and TGFβ1 in the IGF/PI3K/Akt, TGF-β1/Smad3, and VEGF/eNOS/NO pathways.IGFBP-3aggravates inflammatory reactions of the vascular endothelium and liver under hypoxia, affects the normal function of cells, and plays a role in the pathogenesis of diseases.

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Effectiveness of high-dose glucocorticoids on hemolysis, elevating liver enzymes, and reducing platelets syndrome

Cited by 8 publications

References 13 publications

An endolysin Salmcide-p1 from bacteriophage fmb-p1 against gram-negative bacteria

An endolysin Salmcide-p1 from bacteriophage fmb-p1 against gram-negative bacteria

Clinical Classification, Pregnancy Outcomes and Risk Factors Analysis of Severe Preeclampsia Complicated With HELLP Syndrome

Expression of Insulin-Like Growth Factor Binding Protein-3 in HELLP Syndrome

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