Effectiveness of Led Photobiomodulation Therapy on Treatment With Knee Osteoarthritis

Abstract

Objective The present study aimed to evaluate the effectiveness of photobiomodulation therapy by light-emitting diode on osteoarthritis treatment in the knees of rats. Design Twenty male Wistar rats were randomly assigned into two experimental groups: OAC: animals subjected to induction of osteoarthritis, without therapeutic intervention and the group OAL: animals subjected to induction of osteoarthritis treated with light-emitting diode photobiomodulation therapy (850 nm, 200 mW, 6 J). Results The results of gait analysis showed no statistical difference between the groups. The histological findings showed that the OAL group presented abnormal chondrocyte orientation, yet with less irregularities along fibrillation and the joint tissue. Thus, it presented a lower degenerative process when evaluated by the Osteoarthritis Research Society International. Likewise, in the immunohistochemical analysis, the OAL group showed higher collagen 2 and transforming growth factor β immunoexpression when compared with the OAC group. Conclusions Given the above, it is possible to suggest that the photobiomodulation therapy by light-emitting diode had positive effects on the expression of extracellular matrix proteins responsible for synthesis of articular tissue.

In Vitro and In Vivo Effects of Light Therapy on Cartilage Regeneration for Knee Osteoarthritis: A Systematic Review

“…1 . From the initial 1049 records, identified 33 studies (3 in vitro 18 -20 and 30 in vivo 7 -9,21 -47 ) met the eligibility criteria and were included in this systematic review.…”

“…The ECM synthesis and/or degradation after LT varied across the included studies. The expressions of COL II (measured by immunoexpression, 21 Western blot, 22 picrosirius red staining, 31,36 and PCR 34,43 ), GAGs (measured by toluidine blue staining 7 ), and ACAN (measured by PCR 34 ) increased significantly following LT. However, there was one study that revealed no effect on COL II and ACAN expressions (measured by immunoexpression 32 and PCR, 43 respectively).…”

“…The histological outcomes were assessed through the grading of OA, morphometric analysis, and cartilage organization (Supplementary Table S4). The LT significantly decreased the stage of OA, as graded by the Osteoarthritis Research Society International (OARSI) or OARSI modified score, 8,21,29,30 Mankin, 26,41,44 or other score systems. 34,43,46 Only 2 studies 23,37 reported no effect on OA grading after LT.…”

“…The most commonly reported histological effects of LT were the slowing down of chondrocytes proliferation (in number and organization), fewer signs of fibrillation and less irregularities in articular cartilage. 8,21,23,24,26,28,29,32,34 -37,41,46 Three studies 33,36,38 detected local inflammatory signs after the induction of OA in, and 1 study 42 reported the formation of epithelium and new blood vessels after LT.…”

Terapia por fotobiomodulação: mecanismo de ação e importância clínica no tratamento da osteoartrite de joelho

“…Apesar dos resultados promissores e avanços em experimentos que utilizam técnicas de engenharia de tecidos para reparo da cartilagem, sua tradução para a prática clínica permanece um desafio (Xiang et al, 2020) Dentre estes, vêm se destacando mais recentemente a PBMT por laser de baixa intensidade, uma abordagem terapêutica não invasiva e eficiente para o tratamento clínico de diversas lesões musculoesqueléticas (Bjordal et al, 2010;Hegedus et al, 2009). Sabe-se que esta terapia de luz monocromática, interage com fotorreceptores celulares (cromóforos) específicos e estimulam as propriedades bioquímicas das células, dentre as quais, podemos destacar o aumento da respiração celular e oxigenação tecidual, aumento da síntese de DNA, RNA e proteínas reguladoras do ciclo celular, que podem promover a proliferação celular (Hamblin et al, 2013;Trevisan et al, 2020).…”