Effectiveness of low-dose doxycycline (LDD) on clinical symptoms of Sjögren's Syndrome: a randomized, double-blind, placebo controlled cross-over study

Seitsalo, Hubertus; Niemelä, Raija K.; Marinescu-Gava, Magdalena; Vuotila, Tuija; Tjäderhane, Leo; Salo, Tuula

doi:10.1186/1477-5751-6-11

Cited by 27 publications

(18 citation statements)

References 28 publications

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“…Pregnant patients and patients with anticoagulant medication were left out. A total of 44 patients were screened, of which 22 participated in a study where saliva samples were collected [25]. The patients underwent an oral and dental examination.…”

Section: Methodsmentioning

confidence: 99%

Metabolic Profiling of Saliva in Patients with Primary Sj?gren?s syndrome

Mikkonen¹

2012

Metabolomics

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Volume 3 • Issue 3 • 1000128and promising approach to identify biomarkers associated with several disorders such as celiac disease [6,7], leukemia [8], breast cancer [9,10] and oral carcinomas [11]. A variety of different analytical techniques have been used in the metabolic profiling studies: currently the mass spectrometry (MS) has been utilized most frequently. Among MS techniques, especially two-dimensional gel electrophoresis (2D-GE) and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) have mostly been applied to analyze SS saliva samples [12][13][14][15][16].Instead, another powerful metabolic profiling technique, namely nuclear magnetic resonance (NMR) spectroscopy, has remained largely underexplored in saliva analysis. Although some salivary metabolites have been successfully identified and inter-and intrasubject variability has been investigated by using 1 H NMR [17][18][19][20][21] or 13 C NMR spectroscopy [22], well-designed studies aiming to biomarker identification associated to certain health disorder are very rare [23]. AbstractObjective: To investigate the feasibility of 1 H-NMR spectroscopy for metabolic profiling of human saliva samples and to determine whether the concentration of certain salivary metabolites, mainly representing small organic acids and amino acids, differ between patients with primary Sjögren´s syndrome (pSS) and healthy controls.Methods: Stimulated whole-mouth saliva (SWMS) was collected from female pSS patients (n =15, all fulfilling the revised European Community proposed criteria). Salivary flow rate was immediately determined, samples were then frozen and subsequently analyzed by 1 H-NMR spectroscopy in comparison with samples collected from healthy individuals (n=15).Results: From each sample, up to 24 metabolites could be identified and quantified. Choline and taurine concentrations were very significantly higher in the pSS patients compared to healthy controls (p<0.001), but their concentrations correlated negatively with salivary flow rate. Alanine and glycine concentrations were significantly higher (p=0.004, p=0.007, respectively), whereas butyrate (p= 0.034), phenylalanine (p=0.026) and proline (p=0.032) were only slightly higher in pSS saliva samples than in controls.Conclusions: NMR spectroscopy has a potential for quantitative metabolic profiling of saliva samples. NMR spectroscopy is suitable for the analysis of NAAs in saliva and it can bypass the other methods, which are normally suitable for analysis of just one metabolite. Me ta b o lo m ics: O p e n A c ce ss

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Section: Methodsmentioning

confidence: 99%

Metabolic Profiling of Saliva in Patients with Primary Sj?gren?s syndrome

Mikkonen¹

2012

Metabolomics

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“…However, the primary versus secondary distinction does not necessarily correlate with the severity of symptoms, and individuals with secondary SS may experience sicca symptoms as severe as those of primary. SS is slowly progressive, has no cure, and is treated symptomatically …”

Section: Introductionmentioning

confidence: 99%

Voice disorders in Sjögren's syndrome: Prevalence and related risk factors

et al. 2014

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“…[ 21 , 23 ] Studies with oral pilocarpine and doxycycline recruited only female participants. [ 24 , 25 ] All studies were double-masked except for two. [ 25 , 26 ] In summary, oral pilocarpine, cevimeline, lactoferrin, a traditional Chinese medicine (TCM) herb and linoleic acid/ gamma linoleic acid (5/13 systemic modalities) were found to be more effective than placebo or artificial tear in the treatment of dry eye.…”

Section: Systemic Interventionsmentioning

confidence: 99%

Systematic review of randomized controlled trials in the treatment of dry eye disease in Sjogren syndrome

et al. 2017

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Primary Sjögren’s syndrome is an autoimmune disease characterized by dry eye and dry mouth. We systematically reviewed all the randomized controlled clinical trials published in the last 15 years that included ocular outcomes. We found 22 trials involving 9 topical, 10 oral, 2 intravenous and 1 subcutaneous modalities of treatment. Fluoromethalone eye drops over 8 weeks were more effective than topical cyclosporine in the treatment of dry eye symptoms and signs; similarly, indomethacin eye drops over 1 month were more efficacious than diclofenac eye drops. Oral pilocarpine 5 mg twice daily over 3 months was superior to use of lubricants or punctal plugs for treating dry eye, but 5% of participants had gastrointestinal adverse effects from pilocarpine, though none discontinued treatment. In contrast, etanercept, a TNF-alpha blocking antibody, administered as subcutaneous injections twice weekly, did not improve dry eye significantly compared to placebo injections. In conclusion, topical corticosteroids have been shown to be effective in dry eye associated with Sjögren’s syndrome. As some topical non-steroidal anti-inflammatory drugs may be more effective than others, these should be further evaluated. Systemic secretagogues like pilocarpine have a role in Sjögren’s syndrome but the adverse effects may limit their clinical use. It is disappointing that systemic cytokine therapy did not produce encouraging ocular outcomes but participants should have assessment of cytokine levels in such trials, as those with higher baseline cytokine levels may respond better.(229 words)

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Effectiveness of low-dose doxycycline (LDD) on clinical symptoms of Sjögren's Syndrome: a randomized, double-blind, placebo controlled cross-over study

Cited by 27 publications

References 28 publications

Metabolic Profiling of Saliva in Patients with Primary Sj?gren?s syndrome

Metabolic Profiling of Saliva in Patients with Primary Sj?gren?s syndrome

Voice disorders in Sjögren's syndrome: Prevalence and related risk factors

Systematic review of randomized controlled trials in the treatment of dry eye disease in Sjogren syndrome

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