Effectiveness of marker-based selection for Fusarium head blight resistance in spring wheat

Abstract

Abstract:The effect of selection for two donor-QTL from Fusarium head blight (FHB) resistant spring wheat variety Sumai 3 on the reduction of deoxynivalenol (DON) content and FHB index was evaluated in field trials over two years (2008, 2009) following artificial inoculation with Fusarium culmorum. This study was conducted on populations of recombinant inbred lines derived from two crosses, Sumai 3/Swedget and Sumai 3/SG-S 191-01. DON content and FHB index were significantly reduced in both crosses in the genotype classes with two stacked donor QTL on chromosomes 3B and 5A in comparison to genotype classes with no donor QTL. In the cross Sumai 3/Swedget the selection for QTL alleles from 3B and 5A resulted in a 63.4% reduction in DON content, and a 51.8% reduction in the FHB index. Similarly, there was a 35.9% and 31.9% reduction, respectively, in the cross Sumai 3/SG-S 191-01. The single effect of the donor-QTL allele from 3B was significant only in the cross Sumai 3/Swedjet. The presence or absence of awns affected both DON content and FHB index in both populations, but was only significantly in the cross Sumai 3/SG-S 191-01. In this cross the effect of selection for fully awned genotypes was particularly evident on a reduction of both DON and FHB index in classes with neither donor QTL, or the 3B QTL. However, the data indicate that the "awnedness" effect on FHB resistance may be highly variable and is probably greater on reducing FHB symptoms than on DON content. The results confirmed that marker-based introgression of resistance QTLs on chromosomes 3B and 5A in traditional breeding materials can enrich populations for resistance types, but it was also shown that the effect of marker-based selection need not be large in all crosses and a similar effect can probably be reached by indirect selection for some FHB-related traits.

Breeding for Fusarium head blight resistance in wheat—Progress and challenges

“…The Asian spring wheat resistance QTL Fbh1 , Fhb2 , Fhb5/Qfhs‐ifa‐5A and QFhs.nau‐2DL have proven to be effective in spring wheat and winter wheat as well. Chrpova et al (), Salameh et al (), Suzuki et al () and von der Ohe et al () validated effectiveness of Fhb1 and Fhb5 / Qfhs.ifa‐5A in diverse genetic backgrounds. Studies demonstrated that in most cases already the presence of a single QTL significantly improved resistance; however, the presence of both Fhb1 and Fhb5 was more efficient and stable in increasing resistance compared to single QTL carriers.…”

Updating the Breeding Philosophy of Wheat to Fusarium Head Blight (FHB): Resistance Components, QTL Identification, and Phenotyping—A Review

“…The parents of Sumai 3 (Funo from Italy and Taiwanxiaomai from China) were moderate resistant genotypes [44], therefore Fhb1 alone cannot secure high resistance) as supposed in many early papers having 3BS (fhb1) QTL [9]. Chrpova et al [11] reported no significant difference between 3BS and no QTL plants (this could be genotype dependent), but the 3BS+5AS plants showed much better resistance.…”

Fine-mapping of the Fusarium head blight resistance QTL Qfhs.ifa-5A identifies two resistance QTL associated with anther extrusion

“…However, only a few QTL have been validated across studies and solely Fhb1 (Cuthbert et al 2006 ), Fhb2 (Cuthbert et al 2007 ), Fhb4 (Xue et al 2010 ), Fhb5 (Xue et al 2011 ), and Qfhs.ndsu - 3AS (Zhu et al 2016 ) have been subjected to fine-mapping to identify tightly linked markers. Qfhs.ifa - 5A (Buerstmayr et al 2002 , 2003 ) is among the most frequently studied resistance QTL and was validated either individually or in combination with other QTL (Bokore et al 2017 ; Chen et al 2006 ; Chrpova et al 2011 ; Kang et al 2011 ; McCartney et al 2007 ; Salameh et al 2011 ; Suzuki et al 2012 ; Tamburic-Ilincic 2012 ; von der Ohe et al 2010 ). Qfhs.ifa - 5A mapped across the centromere of chromosome 5A within a 1.6 cM QTL support interval flanked by the SSR markers barc186 and wmc805 (Buerstmayr et al 2003 , 2018 ).…”