Staphylococcus aureus is a species of bacteria that causes denture stomatitis which is often found in removable denture wearers. Its management is carried out by eliminating pathogenic bacteria and cleaning dentures regularly. One of the natural ingredients that can be used in such condition is red fruit (Pandanus conoideus Lam) which has active compounds such as flavonoids, alkaloids, steroids and triterpenoids. These active compounds are known to have antibacterial activity. This study aimed to determine the effectiveness of red fruit fraction (Pandanus conoideus Lam) with n-Hexane and ethyl acetate as solvent against Staphylococcus aureus. The method used was the Kirby-Bauer disc diffusion method by testing various concentrations (100%, 90%, 80%, 50%, 25%, 12.5%, 6.25%, and 3.125%) and measuring the inhibition zone diameter by using a caliper. The results showed that the red fruit fraction with ethyl acetate solvent at a concentration of 100% had an average inhibition zone diameter of 8.80 mm classified as medium category, followed by 90% and 80% respectively. In conclusion, ethyl acetate fraction of red fruit (Pandanus conoideus Lam) is effective in inhibiting the growth of Staphylococcus aureus.
Keywords: Staphylococcus aureus; denture stomatitis; removable partial denture, red fruit fraction; inhibition zone
Abstrak: Staphylococcus aureus merupakan salah satu spesies bakteri penyebab denture stomatitis yang sering dijumpai pada pasien pengguna gigi tiruan lepasan. Penatalaksanaan dilakukan dengan mengeliminasi bakteri patogen dan rutin membersihkan gigi tiruan. Salah satu bahan alami yang dapat digunakan ialah buah merah (Pandanus conoideus Lam) yang mengandung senyawa aktif seperti flavonoid, alkaloid, steroid, dan triterpenoid yang diketahui memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui efektivitas fraksi buah merah (Pandanus conoideus Lam) dengan pelarut n-Heksana dan etil asetat terhadap Staphylococcus aureus. Metode yang digunakan ialah metode difusi cakram Kirby-Bauer dengan menguji berbagai konsentrasi fraksi buah merah (100%, 90%, 80%, 50%, 25%, 12,5%, 6,25%, dan 3,125%), dan pengukuran diameter zona hambat dilakukan menggunakan jangka sorong. Hasil penelitian menunjukkan bahwa fraksi buah merah dengan pelarut etil asetat pada konsentrasi 100% memiliki rerata diameter zona hambat tertinggi sebesar 8,80 mm yang tergolong kategori sedang diikuti konsentrasi 90% dan 80%. Simpulan penelitian ini ialah fraksi etil asetat buah merah (Pandanus conoideus Lam) efektif dalam menghambat pertumbuhan Staphylococcus aureus.
Kata kunci: Staphylococcus aureus; denture stomatitis; gigi tiruan sebagian lepasan; fraksi buah merah; zona hambat