Effectiveness of short-contact topical tretinoin in promoting wound healing in db/db mice

Toyama, Takuya; Ohura, Norihiko; Kurita, Masanori; Momosawa, Akira; Harii, Kiyonori

doi:10.1080/02844310601012007

Cited by 7 publications

(7 citation statements)

References 16 publications

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“…A higher density of collagen fibers was observed in aged rats treated with tretinoin than in the control group, results that agree with those of Basak et al and Toyama et al but are in opposition to those of Mühlberg et al Disagreements between the results of the latter study and those of this study were probably because of the difference between incision and excision wounds. In an incision wound, contraction is more important for area reduction than collagen deposition.…”

Section: Discussionsupporting

confidence: 76%

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Effects of tretinoin on wound healing in aged skin

Peseto

Carmona

Silva

et al. 2016

Wound Repair Regeneration

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Aged and adult populations have differences in the structural, biological, and healing properties of skin. Comparative studies of healing under the influence of retinoids in both these populations are very important and, to the best of our knowledge, have not been performed to date. The purpose of this study was to compare the activities of topical tretinoin in aged and adult animal models of wound healing by secondary intention. Male aged rats (24 months old, n = 7) and adult rats (6 months old, n = 8) were used. The rats were assigned to the following groups according to the dates on which wound samples were excised (day 14 or 21 after model creation): treated group, control group, and naive group. Topical application of tretinoin cream was used only on the proximal wound and was applied daily for 7 days. Wound healing areas were measured using metal calipers, and morphological analysis was performed. Slides were stained with Hematoxylin and Eosin, Masson's trichrome, and periodic acid-Schiff stains. Statistical analysis adopted a 5% coefficient for rejection of the null hypothesis. Although aged animals showed skin repair, complete reepithelialization was found on day 21 in some animals of both groups (treated and control). In aged rats, the wound area was significantly smaller in treated wounds than in untreated wounds, resulting in a larger scar area compared with the adult group. When treated wounds were compared, no differences were found between the wound areas in adult and aged rats. As expected, the collagen concentration was higher in normal skin from adult rats than in normal skin from aged animals, but there was no difference when aged skin was treated with tretinoin. These results indicate that tretinoin increases collagen synthesis in aged skin and returns the healing process to a normal state of skin healing.

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Section: Discussionsupporting

confidence: 76%

“…A delay in reepithelialization was described for topical retinoids in large open wounds . However, no delay was found on comparing treated and untreated wounds in this study.…”

Section: Discussioncontrasting

confidence: 63%

Effects of tretinoin on wound healing in aged skin

Peseto

Carmona

Silva

et al. 2016

Wound Repair Regeneration

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“…The db/db mouse is an animal model of diabetes mellitus, and inflammation-mediated experimental wound progression in the model mimics what is seen in diabetic humans. 19,20 Accordingly, we used this model to evaluate the efficacy of topical administration of a p38 kinase inhibitor compared to a Johnson and Johnson product used topically for the treatment of chronic wounds. Promogran™ (oxidized regenerated cellulose/collagen; Johnson and Johnson Medical, Ltd., Ascot, UK) is used clinically as a topical dressing for chronic wounds.…”

Section: Introductionmentioning

confidence: 99%

p38 MAPK inhibition reduces diabetes-induced impairment of wound healing

Higgins¹

2009

DMSOTT

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In healthy tissue, a wound initiates an inflammatory response characterized by the presence of a hematoma, infiltration of inflammatory cells into the wound and, eventually, wound healing. In pathological conditions like diabetes mellitus, wound healing is impaired by the presence of chronic nonresolving inflammation. p38 mitogen-activated protein kinase (MAPK) inhibitors have demonstrated anti-inflammatory effects, primarily by inhibiting the expression of inflammatory cytokines and regulating cellular traffic into wounds. The db/db mouse model of type 2 diabetes was used to characterize the time course of expression of activated p38 during impaired wound healing. The p38α-selective inhibitor, SCIO-469, was applied topically and effects on p38 activation and on wound healing were evaluated. A topical dressing used clinically, Promogran TM , was used as a comparator. In this study, we established that p38 is phosphorylated on Days 1 to 7 post-wounding in db/db mice. Further, we demonstrated that SCIO-469, at a dose of 10 µg/wound, had a positive effect on wound contraction, granulation tissue formation, and re-epithelialization, and also increased wound maturity during healing. These effects were similar to or greater than those observed with Promogran TM . These results suggest a novel approach to prophylactic and therapeutic management of chronic wounds associated with diabetes or other conditions in which healing is impaired.

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“…Effects were assessed with haematoxylin and eosin staining and scanning electron microscopic findings of the internal tissue. Tissues were prepared and observed by the methods described previously [11].…”

Section: Experiments 4: Histological Analyses Of Vascular Pedicle Withmentioning

confidence: 99%

Thermal effect of illumination on microsurgical transfer of free flaps: Experimental study and clinical implications

Kurita

Okazaki

Ozaki

et al. 2008

Scandinavian Journal of Plastic and Reconstructive Surgery and

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The light source of a modern operating microscope gives a clear operative field, but the possibility of thermal impairment by the strong light is substantial. We aimed to elucidate the potential risk of such strong light on transfer of free flaps and the optimal application of light. First, the thermal effect of clinically used microsurgical illumination was examined by direct irradiation of a thermometer. Secondly, the thermal influence on living tissue of microsurgical light was evaluated using a model of vascularised and non-vascularised pedicled groin flaps in rats. Thirdly, the influence of strong light on the transfer of free flaps was investigated using a model of a free groin flap in rats. Fourthly, histological alteration was investigated by a light microscopy and scanning electron microscopy. With direct irradiation of a thermometer, temperatures reached values in excess of 80 degrees C with maximal xenon light illumination from 20 cm. The mode of illumination significantly influenced the results of free flaps. Prolonged irradiation of the vascular pedicle in particular resulted in an increase in the number of failures, supposedly caused by venous occlusion. Bright illumination contributed to efficient microvascular procedures, but we confirmed its potential risk. Our findings may benefit all surgeons engaged in transfer of free flaps, as potential impairment caused by excessive operative irradiation is prevented simply by recognition of its harm.

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Effectiveness of short-contact topical tretinoin in promoting wound healing in db/db mice

Cited by 7 publications

References 16 publications

Effects of tretinoin on wound healing in aged skin

Effects of tretinoin on wound healing in aged skin

p38 MAPK inhibition reduces diabetes-induced impairment of wound healing

Thermal effect of illumination on microsurgical transfer of free flaps: Experimental study and clinical implications

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