Effectiveness of the B subunit of cholera toxin in potentiating immune responses to the recombinant hemagglutinin/adhesin domain of the gingipain Kgp from Porphyromonas gingivalis

Zhang, Ping; Yang, Qiu-Bo; Balkovetz, Daniel F.; Lewis, Janina P.; Clements, John D.; Michalek, Suzanne M.; Katz, Jannet

doi:10.1016/j.vaccine.2005.05.004

Cited by 25 publications

(19 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both adjuvants augmented immune responses to Kgp-HArep following mucosal immunization, which was in agreement with our previous findings [15]. However, in the present study we also delineated differential roles for CD80 and CD86 in the adjuvant activities of CTB and MPL.…”

Section: Discussionsupporting

confidence: 93%

“…Kgp-HArep was purified from the inclusion bodies of E. coli M15 [pREP4] containing the plasmid pQE-rHArep under denaturing conditions by using a His-bind resin column, according to a modification of the manufacturer's instructions (Novagen, Madison, WI), as previously described [15]. The purity of Kgp-HArep was confirmed by analysis of silver stained polyacrylamide gel and by Western blot analysis using a rabbit anti-Kgp-HArep antibody generated in this laboratory.…”

Section: Antigen and Adjuvantsmentioning

confidence: 99%

“…The amount of antigen and adjuvant used was based on preliminary studies. Plasma, saliva and vaginal wash samples were collected, as previously described [15], prior to immunization and at 2-week intervals following the initial immunization. Mice were sacrificed 4 weeks after the second immunization, and spleens and cervical lymph nodes (CLN) were aseptically removed for in vitro assessment of antigen-specific proliferative responses.…”

Section: Immunizations and Sample Collectionsmentioning

confidence: 99%

“…The levels of serum IgG and IgG subclass, salivary IgA and vaginal IgA anti-Kgp-HArep antibody activity were determined by an enzyme-linked immunosorbent assay (ELISA), as previously described [15]. Data were logarithmically transformed and presented as the geometric mean x/÷ standard error of the mean (S.E.M.)…”

Section: Evaluation Of Antibody Responsesmentioning

confidence: 99%

“…Studies have shown that antibodies specific for a sequence present within the HArep consensus were associated with reduced colonization of P. gingivalis in patients with periodontal disease [13], in addition to having an inhibitory effect on P. gingivalis-induced hemagglutination [14]. Our previous studies have demonstrated that the recombinant HArep antigen derived from the gingipain Kgp (Kgp-HArep) was effective in inducing humoral and mucosal immune responses, and antibodies to Kgp-HArep prevented P. gingivalis invasion of epithelial cells in vitro [15]. These findings provide evidence for the potential use of Kgp-HArep in the development of a vaccine against periodontitis.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Role of CD80 and CD86 in host immune responses to the recombinant hemagglutinin domain of Porphyromonas gingivalis gingipain and in the adjuvanticity of cholera toxin B and monophosphoryl lipid A

Zhang

Lewis

Michalek

et al. 2007

Vaccine

Self Cite

View full text Add to dashboard Cite

The gingipains of Porphyromonas gingivalis have been implicated in the virulence of this bacterium, and antibodies to the hemagglutinin/adhesin domain (HArep) of the gingipains have been shown to protect against P. gingivalis colonization. However, the cellular mechanisms involved in host responses to HArep have not been elucidated. The purpose of the present study was to determine the functional role of CD80 and CD86 in mediating systemic and mucosal immune responses to the recombinant HArep derived from the gingipain Kgp (Kgp-HArep) after intranasal (i.n.) immunization. We also investigated the effect of the mucosal adjuvants the B subunit of cholera toxin (CTB) and monophosphoryl lipid A (MPL) on the functional role of the costimulatory molecules for the induction of systemic and mucosal responses to Kgp-HArep Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Antigen and Adjuvantsmentioning

confidence: 99%

Section: Immunizations and Sample Collectionsmentioning

confidence: 99%

Section: Evaluation Of Antibody Responsesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Role of CD80 and CD86 in host immune responses to the recombinant hemagglutinin domain of Porphyromonas gingivalis gingipain and in the adjuvanticity of cholera toxin B and monophosphoryl lipid A

Zhang

Lewis

Michalek

et al. 2007

Vaccine

Self Cite

View full text Add to dashboard Cite

show abstract

Greenhouse and field cultivations of antigen-expressing potatoes focusing on the variability in plant constituents and antigen expression

Mikschofsky

Heilmann

Schmidtke³

et al. 2011

Plant Mol Biol

View full text Add to dashboard Cite

The production of plant-derived pharmaceuticals essentially requires stable concentrations of plant constituents, especially recombinant proteins; nonetheless, soil and seasonal variations might drastically interfere with this stability. In addition, variability might depend on the plant organ used for production. Therefore, we investigated the variability in plant constituents and antigen expression in potato plants under greenhouse and field growth conditions and in leaves compared to tubers. Using potatoes expressing VP60, the only structural capsid protein of the rabbit haemorrhagic disease virus (RHDV), CTB, the non-toxic B subunit (CTB) of the cholera toxin (CTA-CTB(5)) and the marker protein NPTII (neomycinphosphotransferase) as a model, we compare greenhouse and field production of potato-derived antigens. The influence of the production organ turned out to be transgene specific. In general, yield, plant quality and transgene expression levels in the field were higher than or similar to those observed in the greenhouse. The variation (CV) of major plant constituents and the amount of transgene-encoded protein was not influenced by the higher variation of soil properties observed in the field. Amazingly, for specific events, the variability in the model protein concentrations was often lower under field than under greenhouse conditions. The changes in gene expression under environmental stress conditions in the field observed in another event do not reduce the positive influence on variability since events like these should excluded from production. Hence, it can be concluded that for specific applications, field production of transgenic plants producing pharmaceuticals is superior to greenhouse production, even concerning the stability of transgene expression over different years. On the basis of our results, we expect equal or even higher expression levels with lower variability of recombinant pharmaceuticals in the field compared to greenhouse production combined with approximately 10 times higher tuber yield in the field.

show abstract

Oral immunisation of mice with transgenic rice calli expressing cholera toxin B subunit fused to consensus dengue cEDIII antigen induces antibodies to all four dengue serotypes

Kim

et al. 2016

Plant Mol Biol

View full text Add to dashboard Cite

Dengue virus (DENV) infection is an emerging global health threat. DENV consists of four distinct serotypes, necessitating a tetravalent vaccine. In this study, expression of consensus envelope protein domain III (cEDIII) fused to cholera toxin B subunit (CTB) in transgenic rice calli was improved using the luminal binding protein BiP at the N-terminus and the SEKDEL signal sequences at the C-terminus, targeting the recombinant protein to endoplasmic reticulum (ER). We found that the fusion protein showed higher levels of expression when compared to the fusion proteins using rice amylase 3D (RAmy3D) or CTB native signal sequence only. The CTB-cEDIII fusion protein was evaluated as an oral dengue vaccine candidate in mice. Serotype specific systemic IgG antibodies and specific IgA response in feces were detected and furthermore, T cell proliferation and high frequency antibody-secreting B cells were detected in the spleen. These results suggest the possible use of plant-based dengue tetravalent vaccine targeted to the mucosal immune system for induction of systemic and mucosal immune responses to DENV infection.

show abstract

Effectiveness of the B subunit of cholera toxin in potentiating immune responses to the recombinant hemagglutinin/adhesin domain of the gingipain Kgp from Porphyromonas gingivalis

Cited by 25 publications

References 76 publications

Role of CD80 and CD86 in host immune responses to the recombinant hemagglutinin domain of Porphyromonas gingivalis gingipain and in the adjuvanticity of cholera toxin B and monophosphoryl lipid A

Role of CD80 and CD86 in host immune responses to the recombinant hemagglutinin domain of Porphyromonas gingivalis gingipain and in the adjuvanticity of cholera toxin B and monophosphoryl lipid A

Greenhouse and field cultivations of antigen-expressing potatoes focusing on the variability in plant constituents and antigen expression

Oral immunisation of mice with transgenic rice calli expressing cholera toxin B subunit fused to consensus dengue cEDIII antigen induces antibodies to all four dengue serotypes

Contact Info

Product

Resources

About